MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

分子分析细胞成像 (MACI) 核心

• 批准号: 10077912
• 负责人: WILLARD M FREEMAN
• 金额: $ 36.3万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077912
• 关键词: Aging; Animal Model; Applications Grants; Area; Binding; Biochemical; Bioinformatics; Biological; Cells; ChIP-seq; Chromatin; Complement; Consultations; Core Facility; Creativeness; DNA Methylation; DNA-Binding Proteins; Data; Data Analyses; Data Set; Detection; Development; Disease; Epigenetic Process; Experimental Designs; Fostering; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Gene Proteins; Genes; Geroscience; Goals; Growth; Health; Health Sciences; Heterogeneity; Human Resources; Image; Image Analysis; Imaging technology; Individual; Investigation; Label; Laboratories; Light; Link; Microscopy; Mitochondria; Modality; Molecular; Molecular Analysis; Nucleic Acids; Oklahoma; Pathway interactions; Preparation; Process; Program Development; Protein Isoforms; Proteins; Publishing; Regulation; Research; Research Design; Research Personnel; Resources; Role; Scientist; Services; Shock; Structure; Tissue Sample; Training; Training Technics; Transcript; Transmission Electron Microscopy; age related; cellular imaging; design; epigenetic regulation; experience; heteroplasmy; imaging approach; imaging capabilities; imaging study; innovation; insight; instrumentation; interest; knowledge base; molecular imaging; programs; protein expression; recruit; single-cell RNA sequencing; skills; tissue preparation; tool; transcription factor; transcriptome; transcriptome sequencing

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a range of instrumentation, services, and training for rigorous analysis of structure and function through gene expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with extensive scientific experience in these areas who already provide collaborative assistance to individual investigators and have significant experience operating core resources. The MACI Core will offer a range of technical services, and also expert advice in study design, experimental approaches, data analysis and interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA- Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing (ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies, the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and transmission electron microscopy services. A key service will be to help our PJIs and other investigators to develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training seminars and project-specific training will expand the knowledge base and capabilities of Geroscience researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research and foster independence, innovation, and creativity of the individual research programs.

基本的老年科学研究需要分析基因和蛋白质的调控，表达和细胞 本地化先进的分子分析和成像技术为研究人员提供了一套强大的工具 他们的研究，但仪器，经验和技能，充分利用这些工具往往远远超出了他们的研究。 一个实验室的能力分子分析和细胞成像（MACI）核心将提供 一系列的仪器，服务和培训，通过基因的结构和功能的严格分析 表达、基因调控和成像研究。通过这种方式，MACI核心将促进对 与健康和疾病有关的分子和细胞年龄变化。MACI核心将雇用具有以下能力的人员： 在这些领域有着广泛的科学经验，已经为个人提供了合作援助， 调查员，并拥有丰富的核心资源运作经验。MACI核心将提供一系列 技术服务，以及研究设计、实验方法、数据分析和 口译、赠款提案准备和培训。MACI核心将提供先进的分子 基因表达和调控的分析（目标1），包括高通量单细胞RNA-Seq（HT-scRNA- Seq）链特异性RNA-Seq基因表达、高通量qPCR、染色质免疫沉淀测序 （ChIP-Seq）用于DNA结合蛋白。MACI核心的目标2是提供先进的、应用特定的组织 制备、细胞标记、成像和图像分析服务和培训。对于细胞定位研究， MACI核心将提供组织制备和切片，沿着有光、共聚焦、落射荧光， 透射电子显微镜服务一项关键的服务将是帮助我们的PJIs和其他调查人员， 开发先进的实验室特异性细胞标记和成像方法。MACI核心的目标3是提供 培训和先进的分子和细胞数据分析服务。从核酸分离到 组织制备和切片，生物信息学和图像分析，双月培训相结合 研讨会和针对具体项目的培训将扩大老年科学的知识基础和能力 研究人员还将提供有关实验设计、分析和赠款建议的咨询。的 MACI核心，沿着与合作伙伴核心，将有助于发展和持续增长的老年科学研究 培养个人研究项目的独立性、创新性和创造力。