Development of broad nanovaccines targeting diverse coronavirus receptor-binding sites

开发针对不同冠状病毒受体结合位点的广泛纳米疫苗

• 批准号: 10328140
• 负责人: Daniel Kulp
• 金额: $ 124.45万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-22 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10328140
• 关键词: 2019-nCoV; Address; Adjuvant; Antibodies; Antibody Repertoire; Antibody Response; Antigens; Automobile Driving; B-Lymphocytes; Binding; Binding Sites; CD8-Positive T-Lymphocytes; COVID-19 therapeutics; COVID-19 vaccination; COVID-19 vaccine; Coronavirus; Coronavirus Infections; Cryoelectron Microscopy; DNA; DNA Vaccines; Development; Devices; Disease Outbreaks; Dose; Electroporation; Epitopes; Escape Mutant; FDA Emergency Use Authorization; Frequencies; Generations; Genetic; Genetic Recombination; Human; Immune Sera; Immune response; Immunity; Immunization; Immunization Programs; Investigation; Libraries; Middle East Respiratory Syndrome Coronavirus; Modeling; Molecular; Mutagenesis; Mutate; Mutation; Nucleic Acids; Phenotype; Preclinical Testing; Publishing; Regimen; Research Project Grants; SARS coronavirus; SARS-CoV-2 spike protein; Site; Technology; Transgenic Animals; Vaccine Clinical Trial; Vaccines; Variant; Virus; Widespread Disease; animal data; base; betacoronavirus; clinical translation; combat; coronavirus receptor; coronavirus vaccine; cross reactivity; design; experimental study; human coronavirus; immunogenicity; interest; lead candidate; mutant; nanoparticle; nanoparticle delivery; nanovaccine; neutralizing antibody; novel; novel coronavirus; nucleic acid delivery; pandemic disease; pathogenic virus; plasmid DNA; pre-clinical; programs; receptor; receptor binding; synergism; universal coronavirus vaccine; vaccine candidate; vaccine development; vaccinology

Project Summary The first project of our pan-CoV proposal is titled ‘Development of broad nanovaccines targeting diverse coronavirus receptor-binding sites’. Our team’s expertise will culminate in structurally guided coronavirus nanoparticle vaccines to broaden CoV vaccine protection. This proposal utilizes our novel platform to develop potent, RBS-focused nanoparticle vaccines to induce broad protection across CoV lineages, escape mutations and potential pandemic CoVs that are of concern. The project aims are: (1) Create a library of mutants that escape coronavirus immunity (2) Develop RBS-focused nanoparticle vaccines to induce broadly neutralizing antibodies to conserved sites using nucleic acid delivery and (3) Develop vaccine regimens to induce broad immunity and protection across diverse CoVs.

项目摘要 我们泛冠状病毒提案的第一个项目名为“针对不同物种的广泛纳米疫苗的开发” 冠状病毒受体结合部位‘。我们团队的专业知识将在结构引导的冠状病毒中达到顶峰 纳米粒子疫苗扩大冠状病毒疫苗保护范围。这项提议利用我们的新平台 开发强大的、以RBS为重点的纳米颗粒疫苗，以诱导跨冠状病毒谱系的广泛保护， 逃避令人担忧的突变和潜在的大流行冠状病毒。该项目的目标是：(1)创建一个 逃避冠状病毒免疫的突变体库(2)开发针对RBS的纳米颗粒疫苗以诱导 使用核酸传递技术广泛中和针对保守部位的抗体，以及(3)开发疫苗方案 在不同的冠状病毒上诱导广泛的免疫力和保护。