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Ultra-Sensitive Multiplexed Quantification of Proteins Secreted from Single-Cells - Resubmission 01

对单细胞分泌的蛋白质进行超灵敏多重定量 - 重新提交 01

基本信息

批准号：
10326373
负责人：
Savas Tay
金额：
$ 39.53万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10326373
关键词：
ART protein Address Antibodies Area Binding Biological Biological Assay Cells Cellular Assay Complement Confusion DNA Data Detection Development Devices Ensure Event Gene Expression Regulation Growth Factor Hormonal Immune Immunity Individual Insulin Investigation Islet Cell Laboratories Life Malignant Neoplasms Measurement Measures Medical Metabolism Methods Microfluidic Microchips Microfluidics Molecular Organism Peptides Population Protein Secretion Proteins Proteomics Reporting Signal Pathway Signal Transduction Signaling Protein Sorting - Cell Movement Spottings Surface System Techniques Technology Testing Time Work amplification detection base biological information processing biological systems cell type chemokine cytokine detection assay detection method digital experimental study graphical user interface hormonal signals microfluidic technology miniaturize new technology operation pancreatic cancer cells pancreatic juice prototype single cell proteins user friendly software user-friendly wound healing

项目摘要

Ultra-Sensitive Multiplexed Quantification of Proteins Secreted from Single-Cells This proposal aims to develop a powerful new technology for ultra-sensitive quantification of multiple proteins secreted from single-cells. Secreted protein signals coordinate cellular and organism-level functions across all biological systems, and their accurate quantification is crucial for understanding biological information processing in general. Unfortunately, signaling studies have been hindered by poor sensitivity and limited multiplexing ability of current proteomic methods, and the confusion of results due to pooling of multiple cell readouts when conducting population-averaged assays. To address the need to quantify single-cell secreted proteins across different signaling systems, we will develop a new microfluidic technology for ultra-sensitive, multiplexed protein quantification at the single-cell level. We will use our wide-ranging expertise in single-cell assay and microfluidic development and our extensive preliminary data to develop a user-friendly system that will measure multiplexed protein signals from a wide range of cell types, in a time-dependent manner. We will validate our method by quantifying signaling time courses of diverse systems including immune cells, cancer secretion, and hormone signaling. This method will, for the first time, provide the means to gather sensitive, multiplexed single-cell secreted protein measurements, and will be indispensable for understanding signaling in immunity, metabolism, development and cancer.

单细胞分泌蛋白的超灵敏多路定量