Mouse and Human Liver Surgery Core

小鼠和人类肝脏手术核心

• 批准号: 10328211
• 负责人: Fady M Kaldas
• 金额: $ 27.16万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10328211
• 关键词: Allogenic; Animal Model; Animals; Archives; Area; Basic Science; Biological; Biopsy; Blood; Blood specimen; Caring; Clinical; Clinical Data; Clinical Research; Collection; Consent; Cryopreservation; Data; Databases; Development; Equipment; Flushing; Functional disorder; Future; Generations; Glean; Goals; Hepatic; Hepatocyte; Human; Immune; Impairment; In Situ; Individual; Inflammation; Injections; Life; Liquid substance; Liver; Mediating; Microsurgery; Modeling; Mus; Myeloid Cells; Operative Surgical Procedures; Organ Transplantation; Outcome; Patients; Performance; Perioperative Care; Peripheral; Portal vein structure; Postoperative Care; Postoperative Period; Procedures; Process; Regulation; Reperfusion Injury; Reperfusion Therapy; Resolution; Role; Sample Size; Sampling; Serum; Signal Pathway; Stress; Supervision; T-Lymphocyte; Technical Expertise; Techniques; Testing; Therapeutic Intervention; Tissue Sample; Tissues; Transgenic Organisms; Transplant Recipients; Transplantation; Transplantation Surgery; Vent; Warm Ischemia; base; bench to bedside; cost; experience; experimental study; human tissue; immunoregulation; implantation; improved; innovation; insight; isoimmunity; liver biopsy; liver transplantation; mouse model; post-transplant; prevent; programs; prospective; sample collection; screening; surgery outcome; targeted treatment; time interval; transplant model

Core B – Summary Core B of Human and Mouse Liver Surgery will be responsible for patient consent, human sample collection and screening, as well as conducting microsurgery procedures related to mouse models of hepatic IRI and OLT for all three projects in this PPG. The specific aims are as follows: Aim 1. To collect, process, and store human biological samples from OLT patients. Donor liver biopsy tissue and peri-, intra- and post-operative blood and fluid samples from the operative field and blood samples peripherally will be collected. Processing and storage will be centralized. Analysis by request from individual projects will be performed. Core B will also collect and maintain the database with all relevant patient data for the PPG duration. Aim 2. To provide OLT and local liver IRI murine microsurgeries and post-operative care in mice with quality and consistency. The Core will be responsible for conducting all the microsurgical OLT/non-transplant procedures and post- transplant care in mice in all three projects. These procedures are technically demanding, and the Core will assure consistent and reliable surgical outcomes in conducting experiments, as proposed in this PPG. Aim 3. To coordinate experiments among projects and maximize the use of biological samples from human OLT patients and mouse liver transplants/recipients. The Core will coordinate experiments between projects to i/ maximize the use of clinical samples from transplanted patients, and ii/ minimize the numbers and costs of experimental animals for microsurgeries. Aim 4. To store extra-human/mouse biological samples and maintain the archive. The Core will store and record surplus tissue samples (liver grafts, host tissues, serum) and maintain them as an archive for potential future use. Aim 5. To offer other surgical support. Based on the experience, and available equipment, Core B will provide support for additional surgical procedures in mouse liver IRI models, such as i.v., and portal vein injections. Core B will enable collaborating PIs of all three projects to achieve their goals by providing skilled technical and scientific expertise and guidance in the key areas of human and liver transplantation surgery, critical to the successful performance of the PPG.

核心B - 摘要 人类和小鼠肝手术的核心B将负责患者同意，人类样本收集 和筛选，以及进行与肝IRI小鼠模型和肝脏模型有关的显微外科手术程序 在此PPG中的所有三个项目中OLT。具体目的如下：目标1。收集，处理和存储 来自OLT患者的人类生物样品。供体肝活检组织以及术后和术后 将收集来自操作场的血液和流体样本，并收集血液样本。加工 和存储将被集中。将通过各个项目的要求进行分析。核心也将 在PPG持续时间内收集并维护所有相关患者数据的数据库。目标2。提供OLT 以及具有质量和一致性的小鼠的局部肝脏IRI小鼠微生物和术后护理。 核心将负责执行所有显微外科/非移植程序和后 在所有三个项目中，小鼠的移植护理。这些程序在技术上是要求的，核心将 如本PPG所建议的那样，确保进行实验的一致和可靠的手术结果。目标3。 协调项目之间的实验并最大化人类的生物样品的使用 OLT患者和小鼠肝移植/受体。核心将协调实验 i/最大化使用移植患者的临床样本的项目，ii/最小化数字和 实验动物微生物的成本。目标4。存储超人/老鼠生物样品 并保持档案。核心将存储和记录多余的组织样品（肝移植，宿主组织， 血清）并将其保留为潜在使用的档案。目标5。提供其他手术支持。 根据经验和可用设备，核心B将为其他外科手术提供支持 小鼠肝脏IRI模型中的程序，例如i.v.和门户静脉注射。核心B将实现合作 通过提供熟练的技术和科学专业知识， 在人类和肝移植手术的关键领域的指导，对于成功表现至关重要 PPG。