Integrated Cancer Genomics Core

综合癌症基因组学共享资源 综合癌症基因组学共享资源（ICG）致力于提供最先进的 随着这些技术的发展，为杜克癌症研究所（DCI）研究人员提供仪器和方案支持 随着时间ICG扩展以满足DCI需求，包括微生物组，表观遗传和增加单 细胞服务由三个机构指定的核心设施提供：测序和基因组技术 核心（SGT），最近建立的微生物组共享资源（MSR）和分子基因组学核心 （MGC）。通过统一DCI成员的这些现有资源，ICG实现了其提供一站式服务的目标。 访问当代癌症中使用的所有主要研究方案和仪器平台 基因组学研究，包括基因组学、转录组学、微生物研究和表观遗传学。 十多年来，ICG一直保持着提供最新、最先进的基因组和 为DCI成员提供转录组服务。ICG包括由三所杜克学校提供的服务。 医学（SOM）核心设施。测序和基因组技术（SGT）和微生物组共享 资源（MSR）在杜克基因组学和计算生物学中心内提供服务; 分子基因组学核心（MGC）在杜克分子生理学研究所内提供服务。导航卫星委员会 统一了校园内所有的癌症基因组技术，提供一站式访问所有主要研究 现代癌症基因组学研究中使用的协议和仪器平台。ICG支持一个 通过提供专家咨询、项目管理和培训，为DCI调查员提供广泛的项目 促进获得包括SNP发现、绘制染色质修饰、单细胞 测序，在几个尺度上测量mRNA水平（单基因，癌症面板，整个转录组）， 测序外显子组，鉴定DNA甲基化，微生物组分析和绘制转录因子结合图谱 网站.通过提供全方位的技术平台，ICG使调查人员能够选择最佳的 为他们的癌症相关项目提供解决方案，并协助研究人员进行数据质量控制、版本控制、统计 分析和传播所有这些服务。此外，ICG与DCI调查人员合作， 并建立新技术，促进癌症研究的进步。 2018年，ICG共享资源为290名调查人员提供了服务，其中29%是DCI成员， 占总使用量的25%，来自所有8个DCI研究项目。DCI使用此共享资源 在项目期间，成员们发表了216篇论文，其中80篇发表在高影响期刊上。的 共享资源主要在成本回收的基础上运作，并为部分资源提供机构支持 运营成本和仪器采购。来自癌症中心支持补助金的支持使ICG能够提供 DCI成员提供咨询，协助赠款和手稿准备，并优先安排。用户 其他活动的费用遵循医学院的指导方针。

ABSTRACT– INTEGRATED CANCER GENOMICS SHARED RESOURCE The Integrated Cancer Genomics shared resource (ICG) is committed to providing state-of-the-art instrumentation and protocol support to Duke Cancer Institute (DCI) researchers as these technologies evolve over time. The ICG expanded to meet DCI needs that encompass microbiome, epigenetic and increase single cell services provided by three institutionally designated core facilities: Sequencing and Genomic Technologies Core (SGT), the recently established Microbiome Shared Resource (MSR), and the Molecular Genomics Core (MGC). By unifying these existing resources for DCI members, the ICG meets its objective to provide one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research, including genomics, transcriptomics, microbial studies, and epigenetics. For over a decade, the ICG has maintained a record of providing updated, state-of-the-art genomic and transcriptomic services to DCI members. The ICG includes services that are performed by three Duke School of Medicine (SOM) core facilities. The Sequencing and Genomic Technologies (SGT) and Microbiome Shared Resource (MSR) perform services within the Duke Center for Genomics and Computational Biology; the Molecular Genomics Core (MGC) performs services within the Duke Molecular Physiology Institute. The ICG unifies all of the cancer genomic technologies on campus, providing one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research. The ICG supports a wide range of projects from DCI investigators, by providing expert consultation, project management and training to facilitate access to approaches including SNP discovery, mapping chromatin modifications, single-cell sequencing, measuring mRNA levels at several scales (single genes, cancer panels, entire transcriptome), sequencing exomes, identifying DNA methylation, microbiome profiling, and mapping transcription factor binding sites. By offering the full range of technological platforms, the ICG allows investigators to choose the optimal solution for their cancer related projects and assists investigators with data quality control, versioning, statistical analysis, and dissemination for all of these services. In addition, the ICG works with DCI investigators to explore and establish new technologies which catalyzes the advancement of cancer research. In 2018, the ICG shared resource provided services to 290 investigators, 29% of whom were DCI members, accounting for 25% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 216 publications over the project period, 80 of which were in high impact journals. The shared resource operates primarily on a cost-recovery basis, with institutional support for a portion of the operating costs and instrument purchases. Support from the Cancer Center Support Grant allows ICG to provide DCI members with consultations, assistance with grant and manuscript preparation, and scheduling priority. User fees for other activities follow School of Medicine guidelines.