Shared Resource-Flow Cytometry Core

流式细胞仪核心共享资源-项目摘要/摘要 流式细胞仪核心使Salk研究人员能够访问最先进的流式细胞仪仪器， 高质量的服务(确保所有样本都经过适当的处理和分析)和专家 关于设计和实施基于流量的实验的咨询。设施配备齐全 配备两台工作人员操作的荧光激活细胞分类器(FAC)和三台分析仪器，分别是 可独立运行。为具有各种级别体验的用户提供培训和支持，网址为 核心。核心管理数据分析软件的站点许可证，并为用户提供两种分析 工作站。流式细胞术是索尔克研究所广泛使用的一种强大的单细胞技术， 癌症中心成员占总使用量的95%。使用的Flow应用程序示例 癌症中心成员包括：1)表征细胞表型(例如，DNA含量、水平和比率 细胞周期进程、细胞凋亡水平)，2)使用荧光蛋白标记、鉴定和流式细胞仪纯化 来自动物模型的特定类型的癌细胞，以及3)使用荧光抗体标记方法 解析多种细胞类型(例如，确定存在哪些细胞群，包括免疫细胞 在肿瘤微环境中)。后一种技术被应用于极其多样化的 实验，仅受抗体可获得性和检测范围的限制。该设施的员工培养 协作环境，努力为研究所提供易于访问的综合资源 致力于确定对核心用户有用的新兴流量技术或应用。这个 此共享资源的具体目标是通过以下方式促进研究：1)提供仪器-核心 维护一套工具，旨在满足研究所当前和预期的广泛需求 科学家(基于研究发展和趋势)，2)提供专业知识-经验丰富的核心 Salk调查人员可以随时联系到工作人员，以协作开发分析和/或方案，3) 提供关键服务-核心员工提供高质量的实时细胞分选服务，以满足 研究所科学家的不同需求，4)提供教育、培训和支持-索尔克研究人员 专业水平的人员可以接受从实验设计到数据的所有方面的培训 分析，以及5)识别和评估新技术-核心寻求持续确保 提供最先进的流程功能，从而最大限度地提高用户利益。

Flow Cytometry Core Shared Resource - Project Summary/Abstract The Flow Cytometry Core provides Salk investigators access to state-of-the-art flow cytometry instrumentation, high quality services (ensuring that all samples are manipulated and analyzed appropriately), and expert consultation concerning the design and implementation of flow-based experiments. The facility is equipped with two staff-operated fluorescence activated cell sorters (FACS) and three analytical instruments that are available for independent operation. Training and support for users with all levels of experience are available at the Core. The Core manages a site license for data analysis software, and provides users with two analysis workstations. Flow cytometry is a powerful single-cell technique that is widely employed at the Salk Institute, with Cancer Center members accounting for 95% of overall usage. Examples of flow applications used by Cancer Center members include: 1) characterizing cellular phenotypes (e.g., DNA content, levels and rates of cell cycle progression, levels of apoptosis), 2) using fluorescent proteins to label, identify, and FACS purify specific types of cancer cells from animal models, and 3) using fluorescent antibody labeling approaches to resolve multiple cell types (e.g., to determine which populations of cells, including immune cells, are present within the tumor microenvironment). This latter technique is applied to an extremely diverse array of experiments, which is limited only by antibody availability and detection real estate. The facility staff fosters a collaborative environment, striving to provide an easily accessible comprehensive resource to the Institute and dedicates efforts to identify emerging flow technologies or applications that will be useful for Core users. The specific aims of this Shared Resource are to facilitate research by: 1) offering instrumentation – the Core maintains a suite of instruments designed to meet the broad range of current and anticipated needs of Institute scientists (based on research developments and trends), 2) providing expertise – the highly experienced Core staff is readily accessible to Salk investigators for the collaborative development of assays and/or protocols, 3) providing critical services – Core staff provides high-quality, live cell-sorting services that are tailored to meet the varying needs of Institute scientists, 4) providing education, training, and support – Salk researchers at all levels of expertise can receive training in all aspects of flow cytometry, from experimental design to data analysis, and 5) identifying and evaluating new technologies – the Core seeks to continuously ensure that state-of-the-art flow capabilities are offered, thereby maximizing user benefit.