Shared Resource-Flow Cytometry Core

流式细胞术核心共享资源-项目摘要/摘要 流式细胞术核心为索尔克研究人员提供了最先进的流式细胞术仪器， 高质量的服务（确保所有样品都得到适当的操作和分析）， 关于基于流程的实验的设计和实施的咨询。该设施配备了 有两个工作人员操作的荧光激活细胞分选仪（FACS）和三个分析仪器， 可独立操作。为具有各种经验水平的用户提供的培训和支持， 核心的核心管理数据分析软件的站点许可证，并为用户提供两个分析 工作站。流式细胞术是一种强大的单细胞技术，在索尔克研究所广泛使用， 其中癌症中心成员占总使用量的95%。使用的流应用程序示例 癌症中心成员包括：1）表征细胞表型（例如，DNA含量、水平和比率 细胞周期进程、凋亡水平），2）使用荧光蛋白标记、鉴定和FACS纯化 来自动物模型的特定类型的癌细胞，和3）使用荧光抗体标记方法， 解析多种细胞类型（例如，以确定存在哪些细胞群，包括免疫细胞， 肿瘤微环境）。后一种技术适用于各种各样的 实验，这仅受抗体可用性和检测真实的地产的限制。该设施的工作人员培养了一个 协作环境，努力为研究所提供一个容易获得的综合资源， 致力于识别对核心用户有用的新兴流技术或应用。的 该共享资源的具体目标是通过以下方式促进研究：1）提供仪器-核心 维护一套旨在满足研究所当前和预期需求的工具 科学家（基于研究发展和趋势），2）提供专业知识-经验丰富的核心 Salk研究人员随时可以联系到工作人员，以合作开发分析和/或方案，3） 提供关键服务-核心员工提供高质量的活细胞分选服务， 研究所科学家的不同需求，4）提供教育，培训和支持-索尔克研究人员 专业水平的人员可以接受从实验设计到数据的流式细胞术各个方面的培训 5）识别和评估新技术-核心力求不断确保 提供了最先进的流动能力，从而使用户利益最大化。

Flow Cytometry Core Shared Resource - Project Summary/Abstract The Flow Cytometry Core provides Salk investigators access to state-of-the-art flow cytometry instrumentation, high quality services (ensuring that all samples are manipulated and analyzed appropriately), and expert consultation concerning the design and implementation of flow-based experiments. The facility is equipped with two staff-operated fluorescence activated cell sorters (FACS) and three analytical instruments that are available for independent operation. Training and support for users with all levels of experience are available at the Core. The Core manages a site license for data analysis software, and provides users with two analysis workstations. Flow cytometry is a powerful single-cell technique that is widely employed at the Salk Institute, with Cancer Center members accounting for 95% of overall usage. Examples of flow applications used by Cancer Center members include: 1) characterizing cellular phenotypes (e.g., DNA content, levels and rates of cell cycle progression, levels of apoptosis), 2) using fluorescent proteins to label, identify, and FACS purify specific types of cancer cells from animal models, and 3) using fluorescent antibody labeling approaches to resolve multiple cell types (e.g., to determine which populations of cells, including immune cells, are present within the tumor microenvironment). This latter technique is applied to an extremely diverse array of experiments, which is limited only by antibody availability and detection real estate. The facility staff fosters a collaborative environment, striving to provide an easily accessible comprehensive resource to the Institute and dedicates efforts to identify emerging flow technologies or applications that will be useful for Core users. The specific aims of this Shared Resource are to facilitate research by: 1) offering instrumentation – the Core maintains a suite of instruments designed to meet the broad range of current and anticipated needs of Institute scientists (based on research developments and trends), 2) providing expertise – the highly experienced Core staff is readily accessible to Salk investigators for the collaborative development of assays and/or protocols, 3) providing critical services – Core staff provides high-quality, live cell-sorting services that are tailored to meet the varying needs of Institute scientists, 4) providing education, training, and support – Salk researchers at all levels of expertise can receive training in all aspects of flow cytometry, from experimental design to data analysis, and 5) identifying and evaluating new technologies – the Core seeks to continuously ensure that state-of-the-art flow capabilities are offered, thereby maximizing user benefit.