Proteostasis at cellular membranes.

摘要（经修改的R35母公司赔偿额） 我们研究了E3-泛素连接酶调节的蛋白质周转网络在各种膜 哺乳动物细胞系统中的隔室。因此，富集和纯化各种 从哺乳动物细胞的膜隔室是必不可少的了解隔室 特定蛋白质周转。此外，我们对膜蛋白的机制的理解 周转网络及其表型由UPS调节仍然粗略。我们设计 哺乳动物细胞系统与各种细胞遗传技术，以暴露细胞健身 表型，如细胞增殖和存活，受到膜锚定E3- 泛素连接酶。因此，用于测量准确的细胞数量和细胞大小的测定， 在亲本和工程哺乳动物细胞中，对于细胞适应性研究是不可或缺的。

Summary (modified parent R35 award) We investigate E3-ubiquitin ligase regulated protein turnover networks in various membrane compartments in mammalian cell systems. Thus, enrichment and purification of various membrane compartments from mammalian cells is essential for understanding the compartment specific protein turn over. Moreover, our understanding of mechanisms of membrane protein turnover networks and their phenotypes regulated by UPS remains sketchy. We engineer mammalian cell systems with various cell-genetic techniques to expose the cell fitness phenotypes, such as cell proliferation and survival, impacted by membrane anchored E3- ubiquitin ligases. Therefore, assays for the measurements of accurate cell number and cell size, in parent and engineered mammalian cells, are indispensable for the cell fitness studies.