Proteostasis at cellular membranes.

细胞膜的蛋白质稳态。

• 批准号: 10339504
• 负责人: Shafi M. Kuchay
• 金额: $ 5.19万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10339504
• 关键词: Amino Acids; Award; Biological Assay; Biology; Cardiac; Cell Count; Cell Nucleus; Cell Proliferation; Cell Size; Cell Survival; Cell membrane; Cells; Cellular Assay; Cellular Membrane; Communication; Complement; Complex; Cues; Cysteine; Data; Dimethylallyltranstransferase; Disease; Endoplasmic Reticulum; Engineering; Enzymes; Exposure to; F Box Domain; FBXL2 gene; Family; Genetic Techniques; Glare; Goals; Golgi Apparatus; Heart Diseases; Human; Human Genome; Laboratories; Laboratory Research; Lysosomes; Malignant Neoplasms; Mammalian Cell; Measurement; Membrane; Membrane Localization Motif; Membrane Potentials; Membrane Proteins; Mitochondria; Oncoproteins; Orphan; PTEN gene; Parents; Pathway interactions; Pharmaceutical Preparations; Pharmacology; Phenotype; Play; Process; Proteins; Proteolysis; Proteome; Role; Signal Transduction; System; Therapeutic; Tumor Suppressor Proteins; Ubiquitin; Ultracentrifugation; Work; fitness; genetic regulatory protein; interest; multicatalytic endopeptidase complex; nervous system disorder; novel; prenylation; protein degradation; proteostasis; response; ubiquitin ligase; ubiquitin-protein ligase

Summary (modified parent R35 award) We investigate E3-ubiquitin ligase regulated protein turnover networks in various membrane compartments in mammalian cell systems. Thus, enrichment and purification of various membrane compartments from mammalian cells is essential for understanding the compartment specific protein turn over. Moreover, our understanding of mechanisms of membrane protein turnover networks and their phenotypes regulated by UPS remains sketchy. We engineer mammalian cell systems with various cell-genetic techniques to expose the cell fitness phenotypes, such as cell proliferation and survival, impacted by membrane anchored E3- ubiquitin ligases. Therefore, assays for the measurements of accurate cell number and cell size, in parent and engineered mammalian cells, are indispensable for the cell fitness studies.

摘要（经修改的R35母公司赔偿额） 我们研究了E3-泛素连接酶调节的蛋白质周转网络在各种膜 哺乳动物细胞系统中的隔室。因此，富集和纯化各种 从哺乳动物细胞的膜隔室是必不可少的了解隔室 特定蛋白质周转。此外，我们对膜蛋白的机制的理解 周转网络及其表型由UPS调节仍然粗略。我们设计 哺乳动物细胞系统与各种细胞遗传技术，以暴露细胞健身 表型，如细胞增殖和存活，受到膜锚定E3- 泛素连接酶。因此，用于测量准确的细胞数量和细胞大小的测定， 在亲本和工程哺乳动物细胞中，对于细胞适应性研究来说是不可或缺的。