Transgenic Animal Genotyping and Phenotyping Core

项目摘要 转基因动物是生物医学发现的基础，但育种，基因分型， 对于初级研究者和他们的小实验室来说，维持多个转基因品系是繁重的工作。 此外，大多数实验室缺乏完全表征所述细胞所需的专门设备和体内技术。 转基因对心血管基本功能的影响因此，目前的目标是 提案是建立一个新的转基因动物基因分型和表型研究核心（核心B）作为 中央设施，提供这些资源，以支持COBRE的项目领导人和 COBRE心血管研究小组。核心B的具体目标是：（1）建立和运作一个 用于维持转基因动物的集中设施， 心血管系统中的细胞信号转导途径;（2）建立和运行一个设施， 提供评估转基因动物心血管表型的服务;以及（3）提供建议， 为转基因实验动物的使用和鉴定提供培训和技术支持。简言之， 核心B将为项目负责人提供广泛的即用型转基因、基因敲除和 基因编码的生物传感器小鼠可用于研究细胞内的分子和细胞信号转导， 心血管系统此外，核心B将为项目负责人提供一套体内技术， 本发明的目的在于表征其动物模型的心血管表型。这些服务包括遥测 监测血压、心率、血流动力学和运动活动;超声心动图 评估心脏功能;以及用于测量血流的激光多普勒血流仪。成功完成 该建议将减轻维持和表征转基因动物模型的负担。因此，在本发明中， 核心B对于项目领导人的快速进步及其成功过渡到独立至关重要。 此外，在大内华达州地区建立核心B将提供坚实的基础设施和设施 发展一个极具竞争力的心血管研究小组。

Project Summary Genetically modified animals are fundamental for biomedical discovery, but breeding, genotyping, and maintenance of multiple transgenic lines is burdensome for junior investigators and their small laboratories. Further, most labs lack the specialized equipment and in vivo techniques needed to fully characterize the impact of genetic modification on essential cardiovascular functions. Therefore, the goal of the present proposal is to establish a new Transgenic Animal Genotyping and Phenotyping Research Core (Core B) as a centralized facility to provide these resources to support the research of the COBRE’s Project Leaders and the COBRE cardiovascular research group. The specific aims of Core B are to (1) establish and operate a centralized facility for maintaining genetically modified animals used for the investigation of molecular and cellular signal transduction pathways in the cardiovascular system; (2) establish and operate a facility that provides services for evaluating the cardiovascular phenotype of genetically modified animals; and (3) advise, train, and provide technical support for the use and characterization of transgenic laboratory animals. Briefly, Core B will supply the Project Leaders with access to a wide range of ready-to-use transgenic, knockout, and genetically encoded biosensor mice useful for the study of molecular and cellular signal transduction within the cardiovascular system. In addition, Core B will provide Project Leaders with a suite of in vivo techniques for characterization of the cardiovascular phenotypes of their animal models. These services include telemetric monitoring of blood pressure, heart rate, hemodynamics, and locomotor activity; echocardiography for evaluating cardiac function; and laser Doppler flowmetry for measuring blood flow. Successful completion of this proposal will alleviate the burden of maintaining and characterizing transgenic animal models. As such, Core B is vital for the rapid progress of the Project Leaders and their successful transition to independence. Furthermore, establishment of Core B in the greater Nevada area will provide a solid infrastructure and facility for the development of a highly competitive Cardiovascular Research group.