Mouse Phenotyping Research Core

在过去的9年里，作为一个受资助的生物医学研究卓越中心(COBRE)，THRCE 收集了一系列用于评估小鼠心脏和肾脏功能的方法 通过遥测发射器、血压测定进行持续的血流动力学和活动监测 通过体积描记术、单鼠代谢笼研究、肾功能实验、肾神经活动 超声心动图记录心功能。这些测量系统一直得到维护 并作为THRCE-Cobre的核心设施运营，因此成为 THRCE正在进行的许多研究的进展。事实上，这些测量系统现在正在 被杜兰大学的其他研究人员使用，并导致了许多重要的国家和 国际合作。本提案的目标是使这一设施继续作为老鼠 表型研究核心(MPRC)，使其能够为生物医学提供更广泛的功能 THRCE和杜兰大学的研究人员。MPRC将重点关注以下具体事项 目标： 具体目标1：维持和操作最先进的动脉血压评估方法， 高血压及其相关肾脏模型小鼠的神经自主神经状态和肾功能 心血管疾病。 具体目标2：维护和操作心血管超声系统，用于表征小鼠的特征 高血压、肾脏和心血管疾病模型中的心脏和血管功能。 具体目标3：组织和培训调查人员使用这些设施。 MPRC提供的数据收集能力在路易斯安那州是独一无二的。这些功能 对THRCE保持必要的研究卓越至关重要，以将其资金从 科布尔奖授予未来5年和未来的其他竞争性赠款。

For the past 9 years as a funded Center of Biomedical Research Excellence (COBRE), the THRCE has assembled an array of methodologies for assessing cardiac and renal function in mice based on the continuous hemodynamic and activity monitoring by telemetric transmitters, blood pressure determinations by plethysmography, single-mouse metabolic cage studies, renal functional experiments, renal nerve activity recording, and cardiac function by echocardiography. These measurement systems have been maintained and operated as a core facility within the THRCE-COBRE, and as such have become instrumental for the progress of much ongoing research at the THRCE. Indeed, these measurement systems are now being used by other researchers at the Tulane University and have led to a number of significant national and international collaborations. The goal of the present proposal is the continuance of this facility as a Mouse Phenotyping Research Core (MPRC) to allow it to provide a broader range of functions to biomedical researchers within the THRCE and the Tulane University. The MPRC will focus on the following specific aims: Specific Aim 1: To maintain and operate state-of-the-art methodologies for assessing arterial blood pressure, neural autonomic status and renal function in mouse models of hypertension and associated renal and cardiovascular diseases. Specific Aim 2: To maintain and operate a cardiovascular ultrasound system for characterizing mouse cardiac and vascular function in hypertension, renal and cardiovascular disease models. Specific Aim 3: To organize and train investigators in the usage of these facilities. The data collection capabilities offered by the MPRC are unique in the State of Louisiana. These capabilities are vital for the THRCE to maintain the research excellence necessary to transition its funding from the COBRE award to other competitive grants over the next 5 years and into the future.