Mouse Phenotyping Research Core

在过去的9年里，作为生物医学研究卓越中心（COBRE）的资助，THRCE已经 根据小鼠的心脏和肾脏功能， 通过遥测发射器进行连续血液动力学和活动监测， 通过体积描记法、单小鼠代谢笼研究、肾功能实验、肾神经活动 记录和超声心动图检查心脏功能。这些测量系统一直保持着 并作为THRCE-COBRE内的核心设施运营，因此已成为 在THRCE正在进行的许多研究的进展。事实上，这些测量系统现在正在 杜兰大学的其他研究人员使用，并导致了一些重要的国家和 国际合作。本提案的目标是继续使用这一设施， 表型研究核心（MPRC），使其能够提供更广泛的功能，以生物医学 研究人员在THRCE和杜兰大学。该委员会将重点关注以下具体问题： 目的： 具体目标1：维持和操作最先进的动脉血压评估方法， 高血压及相关肾功能损害小鼠模型的神经自主神经状态和肾功能 心血管疾病 具体目标2：维护和操作心血管超声系统，以表征小鼠 高血压、肾脏和心血管疾病模型中的心脏和血管功能。 具体目标3：组织和培训调查人员使用这些设施。 MPRC提供的数据收集能力在路易斯安那州是独一无二的。这些能力 是至关重要的THRCE保持必要的研究卓越过渡其资金从 COBRE奖在未来5年和未来的其他竞争性赠款。

For the past 9 years as a funded Center of Biomedical Research Excellence (COBRE), the THRCE has assembled an array of methodologies for assessing cardiac and renal function in mice based on the continuous hemodynamic and activity monitoring by telemetric transmitters, blood pressure determinations by plethysmography, single-mouse metabolic cage studies, renal functional experiments, renal nerve activity recording, and cardiac function by echocardiography. These measurement systems have been maintained and operated as a core facility within the THRCE-COBRE, and as such have become instrumental for the progress of much ongoing research at the THRCE. Indeed, these measurement systems are now being used by other researchers at the Tulane University and have led to a number of significant national and international collaborations. The goal of the present proposal is the continuance of this facility as a Mouse Phenotyping Research Core (MPRC) to allow it to provide a broader range of functions to biomedical researchers within the THRCE and the Tulane University. The MPRC will focus on the following specific aims: Specific Aim 1: To maintain and operate state-of-the-art methodologies for assessing arterial blood pressure, neural autonomic status and renal function in mouse models of hypertension and associated renal and cardiovascular diseases. Specific Aim 2: To maintain and operate a cardiovascular ultrasound system for characterizing mouse cardiac and vascular function in hypertension, renal and cardiovascular disease models. Specific Aim 3: To organize and train investigators in the usage of these facilities. The data collection capabilities offered by the MPRC are unique in the State of Louisiana. These capabilities are vital for the THRCE to maintain the research excellence necessary to transition its funding from the COBRE award to other competitive grants over the next 5 years and into the future.