Core B: Scientific Core

项目摘要 我们广泛的长期目标是提供试剂，模型和专业知识， 中央科学核心设施的计划项目的所有参与者。核心运作 在根据既定程序提供服务之间取得平衡， 方法和方案的不断创新。安全是一个特殊的指导原则， 核心，并通过核心参与者多年的经验来解决， 朊病毒疾病研究、安全均质化设施和其他生物危害操作 程序朊病毒处理和净化协议标准化是另一个 关键目标，使计划项目的各个方面都能获得试剂， 使用一致的方法生产和运营的服务， 源头科学核心提出了五个具体目标来实现这一目标： 生产和供应特征化的朊病毒病小鼠模型和来自这些模型的组织 Aim II将提供纯化的抗PrP单克隆抗体（mAb）， 目标III将提供纯化的感染性朊病毒制剂;目标IV将量化 朊病毒使用敏感，高通量细胞为基础的测定;和目标V将进行详细的 朊病毒菌株的生物化学、生物物理和结构研究。科学核心有 显示其自身能够适应有效地结合先前核心的要素， 结构，并提供扩展的最先进的设施，技术和技能 整个项目的计划。董事会成员的长期经验。 Telling和Soto博士以及副主任Bian博士是一个重要的力量。科学 核心存在于朊病毒疾病研究的杰出环境中。研究范围 个别项目将与科学核心密切合作， 标准化和一致的模型和专业知识的可用性优势 方法和一致的高质量试剂。科学的活动 核心是成功实现计划目标的关键。

PROJECT SUMMARY Our broad, long-term objectives are to supply reagents, models and expertise from a centralized Science Core facility to all participants of the Program Project. Core operation represents a balance between providing services based on established procedures, and continued innovation in methods and protocols. Safety is a particular guiding principle of the Core, and is addressed by the many years of experience of Core participants in the study of prion diseases, facilities for safe homogenization and other biohazard operating procedures, prion handling and decontamination. Protocol standardization is another crucial goal, so that all aspects of the Program Project have access to reagents and services produced and operated using consistent methods from a constant, reliable source. The Science Core proposes five Specific Aims to accomplish this goal: Aim I will produce and supply characterized mouse models of prion disease and tissues from those mice; Aim II will supply purified anti-PrP monoclonal antibodies (mAbs) with well-defined properties; Aim III will supply preparations of purified infectious prions; Aim IV will quantify prions using sensitive, high throughput cell-based assays; and Aim V will perform detailed biochemical, biophysical and structural studies of prion strains. The Science Core has shown itself to be capable of adapting to efficiently incorporate elements of previous Core structures as well as providing expanded state of the art facilities, techniques and skills for the entire Program Project. The longstanding experience of the Core Co-Directors, Dr. Telling and Dr. Soto, and Associate Director Dr. Bian, is a significant strength. The Science Core resides in outstanding environments for studies of prion diseases. Studies within individual Projects will be conducted in close collaboration with the Science Core to take advantage of the availability of models and expertise, standardized and consistent methodologies, and uniformly consistent, high quality reagents. The activity of the Science Core is essential for the successful accomplishment of Program goals.