Core B: Scientific Core

核心B：科学核心

• 批准号: 10332506
• 负责人: Glenn C Telling
• 金额: $ 42.94万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10332506
• 关键词: Address; Animals; Biochemical; Biohazardous Substance; Biological Assay; Biophysics; Brain; Breeding; Cell Line; Cells; Chronic Wasting Disease; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Collection; Core Facility; Creutzfeldt-Jakob Syndrome; Decontamination; Detection; Detergents; Electron Microscope; Elements; Environment; Enzyme-Linked Immunosorbent Assay; Epitopes; Equilibrium; Flow Cytometry; Fourier Analysis; Gene Targeting; Gene Transfer Techniques; Genotype; Goals; Housing; Human; Immunohistochemistry; Immunoprecipitation; In Vitro; Individual; Infectious Agent; Infrastructure; Knockout Mice; Liquid substance; Maps; Methodology; Methods; Microtus; Modeling; Molecular; Molecular Conformation; Monoclonal Antibodies; Mus; Nature; Participant; Pathogenesis; Polymorph; PrP; PrP variant; PrPSc Proteins; Preparation; Prion Diseases; Prions; Procedures; Property; Proteolysis; Protocols documentation; Reagent; Research Personnel; Resolution; Resources; Rodent Model; Safety; Science; Services; Sheep; Solubility; Source; Specificity; Spectroscopy, Fourier Transform Infrared; Standardization; Structure; System; Techniques; Time; Tissues; Titrations; Transgenic Mice; Transgenic Organisms; Western Blotting; Zoonoses; base; biophysical techniques; cervid; design; experience; experimental study; improved; innovation; mouse model; novel; operation; programs; skills; transmission process

PROJECT SUMMARY Our broad, long-term objectives are to supply reagents, models and expertise from a centralized Science Core facility to all participants of the Program Project. Core operation represents a balance between providing services based on established procedures, and continued innovation in methods and protocols. Safety is a particular guiding principle of the Core, and is addressed by the many years of experience of Core participants in the study of prion diseases, facilities for safe homogenization and other biohazard operating procedures, prion handling and decontamination. Protocol standardization is another crucial goal, so that all aspects of the Program Project have access to reagents and services produced and operated using consistent methods from a constant, reliable source. The Science Core proposes five Specific Aims to accomplish this goal: Aim I will produce and supply characterized mouse models of prion disease and tissues from those mice; Aim II will supply purified anti-PrP monoclonal antibodies (mAbs) with well-defined properties; Aim III will supply preparations of purified infectious prions; Aim IV will quantify prions using sensitive, high throughput cell-based assays; and Aim V will perform detailed biochemical, biophysical and structural studies of prion strains. The Science Core has shown itself to be capable of adapting to efficiently incorporate elements of previous Core structures as well as providing expanded state of the art facilities, techniques and skills for the entire Program Project. The longstanding experience of the Core Co-Directors, Dr. Telling and Dr. Soto, and Associate Director Dr. Bian, is a significant strength. The Science Core resides in outstanding environments for studies of prion diseases. Studies within individual Projects will be conducted in close collaboration with the Science Core to take advantage of the availability of models and expertise, standardized and consistent methodologies, and uniformly consistent, high quality reagents. The activity of the Science Core is essential for the successful accomplishment of Program goals.

项目总结