Core B: Scientific Core
Core B: Scientific Core
批准号:
10332506
负责人:
Glenn C Telling
金额:
$42.94万
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
未结题
起止时间:
2008-08-15 至 2027-02-28
关键词:
AddressAnimalsBiochemicalBiohazardous SubstanceBiological AssayBiophysicsBrainBreedingCell LineCellsChronic Wasting DiseaseClustered Regularly Interspaced Short Palindromic RepeatsCollaborationsCollectionCore FacilityCreutzfeldt-Jakob SyndromeDecontaminationDetectionDetergentsElectron MicroscopeElementsEnvironmentEnzyme-Linked Immunosorbent AssayEpitopesEquilibriumFlow CytometryFourier AnalysisGene TargetingGene Transfer TechniquesGenotypeGoalsHousingHumanImmunohistochemistryImmunoprecipitationIn VitroIndividualInfectious AgentInfrastructureKnockout MiceLiquid substanceMapsMethodologyMethodsMicrotusModelingMolecularMolecular ConformationMonoclonal AntibodiesMusNatureParticipantPathogenesisPolymorphPrPPrP variantPrPSc ProteinsPreparationPrion DiseasesPrionsProceduresPropertyProteolysisProtocols documentationReagentResearch PersonnelResolutionResourcesRodent ModelSafetyScienceServicesSheepSolubilitySourceSpecificitySpectroscopy, Fourier Transform InfraredStandardizationStructureSystemTechniquesTimeTissuesTitrationsTransgenic MiceTransgenic OrganismsWestern BlottingZoonosesbasebiophysical techniquescerviddesignexperienceexperimental studyimprovedinnovationmouse modelnoveloperationprogramsskillstransmission process
中文摘要
项目摘要
我们广泛的长期目标是提供试剂,模型和专业知识,
中央科学核心设施的计划项目的所有参与者。核心运作
在根据既定程序提供服务之间取得平衡,
方法和方案的不断创新。安全是一个特殊的指导原则,
核心,并通过核心参与者多年的经验来解决,
朊病毒疾病研究、安全均质化设施和其他生物危害操作
程序朊病毒处理和净化协议标准化是另一个
关键目标,使计划项目的各个方面都能获得试剂,
使用一致的方法生产和运营的服务,
源头科学核心提出了五个具体目标来实现这一目标:
生产和供应特征化的朊病毒病小鼠模型和来自这些模型的组织
Aim II将提供纯化的抗PrP单克隆抗体(mAb),
目标III将提供纯化的感染性朊病毒制剂;目标IV将量化
朊病毒使用敏感,高通量细胞为基础的测定;和目标V将进行详细的
朊病毒菌株的生物化学、生物物理和结构研究。科学核心有
显示其自身能够适应有效地结合先前核心的要素,
结构,并提供扩展的最先进的设施,技术和技能
整个项目的计划。董事会成员的长期经验。
Telling和Soto博士以及副主任Bian博士是一个重要的力量。科学
核心存在于朊病毒疾病研究的杰出环境中。研究范围
个别项目将与科学核心密切合作,
标准化和一致的模型和专业知识的可用性优势
方法和一致的高质量试剂。科学的活动
核心是成功实现计划目标的关键。
英文摘要
PROJECT SUMMARY
Our broad, long-term objectives are to supply reagents, models and expertise from a
centralized Science Core facility to all participants of the Program Project. Core operation
represents a balance between providing services based on established procedures, and
continued innovation in methods and protocols. Safety is a particular guiding principle of
the Core, and is addressed by the many years of experience of Core participants in the
study of prion diseases, facilities for safe homogenization and other biohazard operating
procedures, prion handling and decontamination. Protocol standardization is another
crucial goal, so that all aspects of the Program Project have access to reagents and
services produced and operated using consistent methods from a constant, reliable
source. The Science Core proposes five Specific Aims to accomplish this goal: Aim I will
produce and supply characterized mouse models of prion disease and tissues from those
mice; Aim II will supply purified anti-PrP monoclonal antibodies (mAbs) with well-defined
properties; Aim III will supply preparations of purified infectious prions; Aim IV will quantify
prions using sensitive, high throughput cell-based assays; and Aim V will perform detailed
biochemical, biophysical and structural studies of prion strains. The Science Core has
shown itself to be capable of adapting to efficiently incorporate elements of previous Core
structures as well as providing expanded state of the art facilities, techniques and skills
for the entire Program Project. The longstanding experience of the Core Co-Directors, Dr.
Telling and Dr. Soto, and Associate Director Dr. Bian, is a significant strength. The Science
Core resides in outstanding environments for studies of prion diseases. Studies within
individual Projects will be conducted in close collaboration with the Science Core to take
advantage of the availability of models and expertise, standardized and consistent
methodologies, and uniformly consistent, high quality reagents. The activity of the Science
Core is essential for the successful accomplishment of Program goals.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Maximizing research success in studies of naturally-occurring prion diseases
-
批准号:10665211
-
项目类别:
-
资助金额:$47.12万
-
财政年份:2023
-
负责人:Glenn C Telling
-
依托单位:
Addressing the mechanisms of prion strain evolution and its effect on interspecies transmission
-
批准号:10378707
-
项目类别:
-
资助金额:$47.69万
-
财政年份:2021
-
负责人:Glenn C Telling
-
依托单位:
Addressing the mechanisms of prion strain evolution and its effect on interspecies transmission
-
批准号:10185649
-
项目类别:
-
资助金额:$51.36万
-
财政年份:2021
-
负责人:Glenn C Telling
-
依托单位:
Addressing the mechanisms of prion strain evolution and its effect on interspecies transmission
-
批准号:10533808
-
项目类别:
-
资助金额:$47.69万
-
财政年份:2021
-
负责人:Glenn C Telling
-
依托单位:
Characterizing the strain and host range properties of prions causing emergent forms of chronic wasting disease
-
批准号:10208982
-
项目类别:
-
资助金额:$46.9万
-
财政年份:2018
-
负责人:Glenn C Telling
-
依托单位:
Characterizing the strain and host range properties of prions causing emergent forms of chronic wasting disease
-
批准号:10434063
-
项目类别:
-
资助金额:$46.9万
-
财政年份:2018
-
负责人:Glenn C Telling
-
依托单位:
Characterizing the strain and host range properties of prions causing emergent forms of chronic wasting disease
-
批准号:9789386
-
项目类别:
-
资助金额:$49.6万
-
财政年份:2018
-
负责人:Glenn C Telling
-
依托单位:
Fyn activation in prion disease
-
批准号:9092646
-
项目类别:
-
资助金额:$22.61万
-
财政年份:2016
-
负责人:Glenn C Telling
-
依托单位:
Fyn activation in prion disease
-
批准号:9234083
-
项目类别:
-
资助金额:$18.97万
-
财政年份:2016
-
负责人:Glenn C Telling
-
依托单位:
Core B: Scientific Core
-
批准号:10589820
-
项目类别:
-
资助金额:$58.57万
-
财政年份:2008
-
负责人:Glenn C Telling
-
依托单位:
Science Core B
-
批准号:9001746
-
项目类别:
-
资助金额:$35.67万
-
财政年份:2008
-
负责人:Glenn C Telling
-
依托单位:
Project 1: Modeling the Mechanisms of Prion Transmission, Strain Selection, Mutation and Species Barrier in Transgenic Mice
-
批准号:10332507
-
项目类别:
-
资助金额:$42.94万
-
财政年份:2008
-
负责人:Glenn C Telling
-
依托单位:
Project 1: Modeling the Mechanisms of Prion Transmission, Strain Selection, Mutation and Species Barrier in Transgenic Mice
-
批准号:10589824
-
项目类别:
-
资助金额:$51.92万
-
财政年份:2008
-
负责人:Glenn C Telling
-
依托单位:
TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
-
批准号:7255648
-
项目类别:
-
资助金额:$31.83万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
-
批准号:6495507
-
项目类别:
-
资助金额:$75.0万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
-
批准号:6394490
-
项目类别:
-
资助金额:$28.96万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
-
批准号:6968578
-
项目类别:
-
资助金额:$33.58万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
-
批准号:6167349
-
项目类别:
-
资助金额:$29.0万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
Transgenetic studies of prion disease in cervids
-
批准号:8492169
-
项目类别:
-
资助金额:$42.57万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
Transgenetic studies of prion disease in cervids
-
批准号:8146016
-
项目类别:
-
资助金额:$45.31万
-
财政年份:2000
-
负责人:Glenn C Telling
-
依托单位:
海外基金