Core B: Scientific Core

项目总结 我们广泛的、长期的目标是从一个 为计划项目的所有参与者提供集中的科学核心设施。核心运营 代表在根据既定程序提供服务和 在方法和协议方面不断创新。安全是一项特殊的指导原则 核心，并通过核心参与者的多年经验在 Pron病、安全均质设备和其他生物危害操作的研究 程序、Pron处理和去污。协议标准化是另一个 关键目标，以便计划项目的所有方面都可以获得试剂和 使用持续、可靠的方法生产和运营的服务 消息来源。科学核心提出了实现这一目标的五个具体目标：目标我将 生产和提供具有特征性的普恩病毒病小鼠模型和这些组织 小鼠；AIM II将提供纯化的抗PrP单抗(MAbs) 性质；AIM III将提供纯化的传染性普恩的制剂；AIM IV将量化 使用灵敏的、高通量的基于细胞的分析；Aim V将执行详细的 Pron菌株的生化、生物物理和结构研究。《科学核心》有 证明自己能够适应有效地整合以前核心的元素 结构，并提供最先进的设施、技术和技能 用于整个计划项目。核心联席董事的长期经验，Dr。 Tell和索托博士，以及副主任边博士，是一个重要的优势。《科学》 CORE位于普恩病毒疾病研究的优秀环境中。范围内的研究 个别项目将与科学核心密切合作，以采取 利用模型和专业知识的可用性、标准化和一致性 方法，以及统一一致的高质量试剂。科学的活跃性 核心是成功实现计划目标的关键。

PROJECT SUMMARY Our broad, long-term objectives are to supply reagents, models and expertise from a centralized Science Core facility to all participants of the Program Project. Core operation represents a balance between providing services based on established procedures, and continued innovation in methods and protocols. Safety is a particular guiding principle of the Core, and is addressed by the many years of experience of Core participants in the study of prion diseases, facilities for safe homogenization and other biohazard operating procedures, prion handling and decontamination. Protocol standardization is another crucial goal, so that all aspects of the Program Project have access to reagents and services produced and operated using consistent methods from a constant, reliable source. The Science Core proposes five Specific Aims to accomplish this goal: Aim I will produce and supply characterized mouse models of prion disease and tissues from those mice; Aim II will supply purified anti-PrP monoclonal antibodies (mAbs) with well-defined properties; Aim III will supply preparations of purified infectious prions; Aim IV will quantify prions using sensitive, high throughput cell-based assays; and Aim V will perform detailed biochemical, biophysical and structural studies of prion strains. The Science Core has shown itself to be capable of adapting to efficiently incorporate elements of previous Core structures as well as providing expanded state of the art facilities, techniques and skills for the entire Program Project. The longstanding experience of the Core Co-Directors, Dr. Telling and Dr. Soto, and Associate Director Dr. Bian, is a significant strength. The Science Core resides in outstanding environments for studies of prion diseases. Studies within individual Projects will be conducted in close collaboration with the Science Core to take advantage of the availability of models and expertise, standardized and consistent methodologies, and uniformly consistent, high quality reagents. The activity of the Science Core is essential for the successful accomplishment of Program goals.