Mouse Genetics Laboratory Shared Resource

小鼠遗传学实验室共享资源摘要 小鼠遗传学实验室(MGL)共享资源的使命是提供最先进的服务 为用户生成新的鼠标模型。转基因小鼠一直是癌症研究的重要工具 多年来，使研究人员能够确定特定基因在癌症相关特征中的作用，如 免疫逃避、肿瘤启动和转移。MGL生产转基因和基因敲除小鼠，冷冻保存 小鼠精子和胚胎，进行胚胎干细胞基因打靶，协助小鼠胚胎 操控，并提供科学咨询。这些服务为共济会癌症中心(MCC) 成员方便、经济高效地获得转基因小鼠和相关技术，并 对正在进行的遗传机制、免疫学和细胞机制计划的工作至关重要 MCC。作为一项新服务，MGL现在直接协调CRISPR/Cas9改变小鼠的生产 基因组工程共享资源(GESR)。Gesr协助研究人员设计和生成 引导RNA并验证其有效性，然后MGL用编辑后的基因组产生小鼠。 MGL现在由Timothy Hallstrom(CM)博士领导，他在老鼠遗传学研究方面有10年的经验。 他是NCI R01的PI，领导着一个专注于控制细胞机制的研究实验室 视网膜母细胞瘤(RB)蛋白。MGL的日常运营由Yun You博士管理，他曾 之前是斯隆纪念医院小鼠遗传核心设施转基因小组的监督员 凯特琳癌症中心，于2016年被招募，负责监督MGL的运作并教育 向实验室用户介绍转基因小鼠品系的政策、方案和维护。 在上一次资助期间，MGL搬到了新的癌症和心血管研究大楼。这个地方 接近生物医学发现区大量研究人员的资源，包括 MCC和学术健康中心团体的成员，如干细胞研究所、 翻译神经科学和利莱黑心脏研究所。

Mouse Genetics Laboratory Shared Resource Summary The mission of the Mouse Genetics Laboratory (MGL) Shared Resource is to provide state-of-the-art services in generating new mouse models for users. Genetically modified mice have been vital tools for cancer studies for many years, enabling researchers to determine the roles of specific genes in cancer-relevant traits such as immune evasion, tumor initiation, and metastasis. MGL produces transgenic and knockout mice, cryopreserves mouse sperm and embryos, performs embryonic stem cell gene targeting, assists with mouse embryo manipulation, and provides scientific consultation. These services provide Masonic Cancer Center (MCC) members convenient, cost-effective access to genetically modified mice and associated technologies and are critical to the ongoing work of the Genetic Mechanisms, Immunology, and Cellular Mechanisms Programs of the MCC. As a new service, MGL now directly coordinates production of CRISPR/Cas9-altered mice with the Genome Engineering Shared Resource (GESR). GESR assists researchers with the design and generation of guide RNA and validates their efficacy, and then MGL generates mice with the edited genome. MGL is now led by Dr. Timothy Hallstrom (CM) who has 10 years of experience with mouse genetics research. He is the PI of an NCI R01 and leads a research laboratory focused on the cellular mechanisms controlling the retinoblastoma (Rb) protein. Day-to-day operations of the MGL are managed by Yun You, PhD, who was previously the supervisor of the Transgenic Group in the Mouse Genetic Core Facility at Memorial Sloan Kettering Cancer Center and was recruited in 2016 to supervise the operations of the MGL and to educate users of the laboratory on policies, protocols, and maintenance of transgenic mouse strains. In the last funding period, MGL moved to the new Cancer and Cardiovascular Research Building. This places the resource in close proximity to a large number of investigators in the Biomedical Discovery District, including members of the MCC and Academic Health Center groups such as the Stem Cell Institute, Institute for Translational Neuroscience, and Lillehei Heart Institute.