Mouse Genetics Laboratory Shared Resource

小鼠遗传学实验室共享资源

• 批准号: 10333244
• 负责人: Timothy C. Hallstrom
• 金额: $ 8.61万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10333244
• 关键词: Acute Myelocytic Leukemia; B lymphoid malignancy; CRISPR/Cas technology; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cardiovascular system; Colorectal Cancer; Communities; Consultations; Core Facility; Cryopreservation; Development; Doctor of Philosophy; Embryo; Family; Funding; Gene Targeting; Generations; Genes; Genetic; Genetic Research; Genetic Screening; Genetic Transcription; Genome engineering; Glioma; Guide RNA; Health; Heart; Home; Immune Evasion; Immunity; Immunology; Institutes; Knockout Mice; Laboratories; Laboratory Research; Maintenance; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Mission; Modeling; Mouse Strains; Mus; Neoplasm Metastasis; Peripheral Nerve Sheath Neoplasm; Policies; Production; Protocols documentation; Research; Research Personnel; Resource Sharing; Resources; Retinoblastoma Protein; Role; Schwann Cells; Screening for cancer; Services; Supervision; Surveillance Methods; System; T-Cell Development; T-Cell Leukemia; Technology; Testicular Neoplasms; Transgenic Mice; Transgenic Organisms; Tumor Immunity; Universities; Work; anticancer research; base; cost effective; design; embryonic stem cell; experience; genome editing; in vivo; malignant breast neoplasm; member; mouse genetics; mouse model; operation; osteosarcoma; programs; recruit; sperm cell; stem cell genes; stem cells; tool; trait; translational neuroscience; tumor; tumor initiation

Mouse Genetics Laboratory Shared Resource Summary The mission of the Mouse Genetics Laboratory (MGL) Shared Resource is to provide state-of-the-art services in generating new mouse models for users. Genetically modified mice have been vital tools for cancer studies for many years, enabling researchers to determine the roles of specific genes in cancer-relevant traits such as immune evasion, tumor initiation, and metastasis. MGL produces transgenic and knockout mice, cryopreserves mouse sperm and embryos, performs embryonic stem cell gene targeting, assists with mouse embryo manipulation, and provides scientific consultation. These services provide Masonic Cancer Center (MCC) members convenient, cost-effective access to genetically modified mice and associated technologies and are critical to the ongoing work of the Genetic Mechanisms, Immunology, and Cellular Mechanisms Programs of the MCC. As a new service, MGL now directly coordinates production of CRISPR/Cas9-altered mice with the Genome Engineering Shared Resource (GESR). GESR assists researchers with the design and generation of guide RNA and validates their efficacy, and then MGL generates mice with the edited genome. MGL is now led by Dr. Timothy Hallstrom (CM) who has 10 years of experience with mouse genetics research. He is the PI of an NCI R01 and leads a research laboratory focused on the cellular mechanisms controlling the retinoblastoma (Rb) protein. Day-to-day operations of the MGL are managed by Yun You, PhD, who was previously the supervisor of the Transgenic Group in the Mouse Genetic Core Facility at Memorial Sloan Kettering Cancer Center and was recruited in 2016 to supervise the operations of the MGL and to educate users of the laboratory on policies, protocols, and maintenance of transgenic mouse strains. In the last funding period, MGL moved to the new Cancer and Cardiovascular Research Building. This places the resource in close proximity to a large number of investigators in the Biomedical Discovery District, including members of the MCC and Academic Health Center groups such as the Stem Cell Institute, Institute for Translational Neuroscience, and Lillehei Heart Institute.

小鼠遗传学实验室共享资源摘要 小鼠遗传学实验室（MGL）共享资源的使命是提供最先进的服务 为用户创造新的鼠标模型。转基因小鼠一直是癌症研究的重要工具 多年来，使研究人员能够确定特定基因在癌症相关性状中的作用， 免疫逃避、肿瘤起始和转移。MGL生产转基因和基因敲除小鼠，冷冻保存 小鼠精子和胚胎，进行胚胎干细胞基因靶向，协助小鼠胚胎 操作，并提供科学咨询。共济会癌症中心（MCC） 成员方便，具有成本效益的获得转基因小鼠和相关技术， 关键的遗传机制，免疫学和细胞机制计划的正在进行的工作， MCC。作为一项新服务，MGL现在直接协调CRISPR/Cas9改造小鼠的生产， 基因组工程共享资源（GESR）。GESR帮助研究人员设计和生成 指导RNA并验证其功效，然后MGL产生具有编辑的基因组的小鼠。 MGL现在由拥有10年小鼠遗传学研究经验的Timothy Hallstrom博士（CM）领导。 他是NCI R01的PI，并领导一个研究实验室，专注于控制细胞的细胞机制。 视网膜母细胞瘤（Rb）蛋白。MGL的日常运营由Yun You博士管理， 他以前是斯隆纪念医院小鼠遗传核心设施转基因组的主管 Kettering癌症中心，并于2016年招募，以监督MGL的运作，并教育 向实验室用户提供转基因小鼠品系的政策、方案和维护方面的信息。 在上一个资助期，MGL搬到了新的癌症和心血管研究大楼。这使 该资源靠近生物医学发现区的大量研究人员，包括 MCC和学术健康中心团体的成员，如干细胞研究所， 翻译神经科学和Lillehei心脏研究所。