OspC and its role in defining host range and dissemination properties

OspC 及其在定义主机范围和传播属性中的作用

• 批准号: 10345736
• 负责人: RICHARD T MARCONI
• 金额: $ 57.78万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10345736
• 关键词: Affinity; Alleles; Amino Acid Substitution; Binding; Biological; Birds; Borrelia; Borrelia afzelii; Borrelia burgdorferi; Borrelia burgdorferi Group; Borrelia garinii; Charge; Complement; Complement 4b; Coupled; Data; Distal; Electrostatics; Extracellular Matrix; Genome; Human; Incidence; Individual; Infection; Ixodes; Kinetics; Ligand Binding; Ligand Binding Domain; Ligands; Link; Lyme Disease; Maintenance; Mammals; Mediating; Membrane; Membrane Proteins; Molecular; Mus; Mutate; Organ; Oryctolagus cuniculus; OspC protein; Outcome; Pathogenesis; Pathogenicity; Plasminogen; Play; Positioning Attribute; Preventive; Property; Protein C; Proteins; Public Health; Recombinant Proteins; Research; Role; Site-Directed Mutagenesis; Solvents; Structure; Testing; Therapeutic; Tick-Borne Diseases; Ticks; Transgenic Organisms; Variant; Virulence Factors; Work; antigen diagnostic; base; citrate carrier; companion animal; dimer; enzootic; genetic approach; improved; in vivo; innovation; mutant; pathogen; tick population; tick transmission; tissue tropism; vaccine development

Project summary: Lyme disease is the most common tick-borne disease in the northern hemisphere. The incidence of Lyme disease is steadily increasing as the Ixodes tick population expands. This proposal will identify the critical functional determinants of outer surface protein C (OspC), an essential virulence factor. In addition, we will determine how sequence variation in OspC influences infectivity and dissemination properties. Cutting edge genetic approaches will be employed to dissect the role that OspC plays in the pathobiology of the Lyme disease spirochetes. The outcome of these analyses will also advance ongoing efforts to develop vaccines and diagnostic antigens for use in humans.

项目概述：莱姆病是北方最常见的蜱传疾病 半球莱姆病的发病率随着硬蜱种群的增加而稳步上升， 扩展。这一建议将确定外表面蛋白C的关键功能决定因素 OspC是一种重要的毒力因子。此外，我们将确定序列变异如何在 OspC影响传染性和传播特性。尖端的遗传学方法将 用于剖析OspC在莱姆病病理生物学中的作用 螺旋体这些分析的结果还将推动正在进行的努力， 用于人类的疫苗和诊断抗原。