Acoustofluidic Separation (AFS), Purification and Raman Spectral Fingerprinting of Single EVs: From Cell of Origin to Target Cell and Biofluids
单个 EV 的声流分离 (AFS)、纯化和拉曼光谱指纹识别:从起源细胞到目标细胞和生物流体
基本信息
- 批准号:10357985
- 负责人:
- 金额:$ 89.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-05 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:ApoptoticArea Under CurveBiological MarkersBloodCancer DetectionCellsClinicalCommunicationCommunication ProgramsCommunication ResearchCommunitiesCouplingCredentialingDetectionDevelopmentDiagnosticDiseaseERCC2 geneEarly DiagnosisFingerprintFoundationsFundingGoalsHeterogeneityHomingHumanImmunoblottingLabelMalignant NeoplasmsManuscriptsMetabolic DiseasesMolecular AnalysisNon-Invasive Cancer DetectionOrganOutcomePerformancePhasePreparationProceduresProtocols documentationPublic HealthPublishingRNARaman Spectrum AnalysisReproducibilitySalivaSalivarySalivary GlandsSeminalSorting - Cell MovementSourceSpecificitySurfaceSystemSystemic diseaseTSG101 geneTechnologyTestingTherapeuticTissuesUnited States National Institutes of Healthbasebiomarker developmentexosomeextracellularextracellular vesicleshuman tissueinnovative technologiesliquid biopsymalignant stomach neoplasmmicrovesiclesnervous system disordernew technologynovelscale uptool
项目摘要
Project Summary/Abstract:
This UG3/UH3 application is responsive to the NIH Common Fund initiative RFA-RM-18-028 “Advancing
Extracellular RNA (exRNA) Communication Research: Towards Single Extracellular Vesicle (EV) Sorting,
Isolation, and Analysis of Cargo”. This proposal is built on a foundation of a 5-year NIH Common Fund
“Extracellular RNA Communication Consortium Stage 1 (ERCC1)” project to develop salivary exRNA biomarkers
for gastric cancer detection where a panel of highly discriminatory salivary extracellular RNA (exRNAs) have
been developed (discovered and definitively validated) for gastric cancer, scientifically and translationally
credentialed salivary exRNA for systemic disease detection.
This ERCC Stage 2 project is to develop an innovative technology, AcoustoFluidic Separation (AFS) coupling
with Surface Enhanced Raman Spectroscopy (SERS), towards single EV isolation and to characterize exRNA
cargos associated with specific EV subpopulations based on the cells of origin, intended target cells and biolfuids.
Eight Specific Aims with twelve quantitative milestones in two phases (UG3, UH3) are in place to test the
central hypothesis that exosomes are the EV from gastric cancer tissue/cells of origin harboring the nine
validated discriminatory salivary exRNA biomarkers, transported through vasculature, homing into salivary
glands (target organ) and into saliva. Four Specific Aims in the UG3 Phase are to develop the AFS technology
as a standard operating procedure (SOP) for rigor and reproducibility (Aim 1); SERS development for EV
fingerprinting and co-localization of exRNA targets to singe EV (Aim 2); two independent “Rigor and
Reproducibility Labs (R&R Labs)” to evaluate the AFS SOP (Aim 3) and approaches to share strategies,
protocols, tools with broader scientific community with DMRR (Aim 4). In the UH3 Phase four Specific Aims are
in place to optimize, refine and scale up the AFS SOP (Aim 5); perform sorting, tracking of validated salivary
exRNA biomarkers for gastric cancer detection from cells of origin, to blood, to salivary glands and to saliva (Aim
6); “R&R Labs” to optimize AFS to other human biofluids (Aim 7) and approaches to share strategies, protocols,
tools with broader scientific community with DMRR (Aim 8).
Completing these Aims and goals based on the outcome of the ERCC1 project is logical and highly impactful
as the outcomes of the ERCC2 project will deliver a set of novel technologies, AFS in tandem with SERC, for
rapid, high yield (6X over current technologies) and single EV level isolation for salivary biomarker development
for systemic disease detection. These will constitute the foundation of “exRNA Saliva Liquid Biopsy (exRNA-
SLB)” where the diagnostic and therapeutic functionality of exRNAs can be fully realized when the range of EV
subpopulations from a given cell source can be characterized and analyzed for molecular cargos.
项目总结/文摘:
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Ji Yeong An其他文献
Ji Yeong An的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Ji Yeong An', 18)}}的其他基金
Acoustofluidic Separation (AFS), Purification and Raman Spectral Fingerprinting of Single EVs: From Cell of Origin to Target Cell and Biofluids
单个 EV 的声流分离 (AFS)、纯化和拉曼光谱指纹识别:从起源细胞到目标细胞和生物流体
- 批准号:
10009490 - 财政年份:2019
- 资助金额:
$ 89.39万 - 项目类别:














{{item.name}}会员




