The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 2

密苏里大学突变小鼠资源和研究中心 - 应用研究第 2 部分

基本信息

  • 批准号:
    10360486
  • 负责人:
  • 金额:
    $ 4.6万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-05-01 至 2025-02-28
  • 项目状态:
    未结题

项目摘要

Applied Research section 2 – Project Summary Following identification of associations between characteristics of a complex gut microbiota (GM) and resistance or susceptibility to disease, investigators frequently attempt to evaluate the causality of those associations by transferring the GM between resistant and susceptible animals. There are several ways to do so including embryo transfer of GM recipients into pseudopregnant GM donors, cross-fostering of neonatal GM recipients to nursing GM donors, simply co-housing animals, or experimentally administering a slurry prepared from donor feces to germ-free or antibiotic-treated GM recipients (fecal microbiota transfer, FMT). Whether successful or not in transferring the phenotype, few studies closely examine or report the efficiency of GM transfer from donor to recipient, and very few studies directly compare more than one method of GM transfer. Moreover, differences in the microbial diversity within donor and recipient GMs are known to influence the success of GM transfer. Thus the long-term objectives of Applied Research section 2 (Specific Aim 1) are to compare the efficiency of GM transfer using the aforementioned methods and well-controlled and thoroughly characterized GM recipients and donors harboring low and high-diversity GM profiles. Specific Aim 1 is to test the efficiency of GM transfer using embryo transfer, cross-fostering, co-housing, and FMT, in reciprocal transfers between low-diversity GM donors and high-diversity recipients and vice versa, and to determine whether the degree of GM transfer is associated with the degree to which the phenotype is also transferred. Furthermore, while feces represents a non-invasive sample allowing for longitudinal sampling of individual mice, it is well-recognized that feces reflects only the lower gastrointestinal tract (GIT). Many physiological processes and disease mechanisms are dependent on the GM present in the upper GIT, necessitating multiple terminal cohorts of mice for longitudinal studies as non-invasive ante mortem sampling of the upper GIT is not currently possible. With that in mind, the long-term objective of Applied Research section 2 (Specific Aim 2) is to develop and validate a system of non-invasively sampling the upper GIT for downstream sequencing methods. Working with a team of biomaterials engineers, Specific Aim 2 to develop a polymeric/metallic particle-based system capable of collecting bacterial cells selectively from the upper GIT following oral gavage, and then being retrieved and isolated from feces for downstream molecular analyses. The development of such a system would advance the three ‘R’s of comparative medicine by reducing the required number of mice for longitudinal studies examining the upper GIT. Moreover, these methods would revolutionize human medicine and would be imminently patentable and likely to lead to subsequent funding via multiple mechanisms. Collectively, the Aims of Applied Research section 2 will significantly enhance the ability of researchers from a wide range of fields to manipulate the GM of laboratory mice in an informed manner, and to sample regions of the GIT that were previously inaccessible.
应用研究第2组-计划摘要 在确定复杂肠道微生物群(GM)的特征与 研究人员经常试图评估这些疾病的因果关系, 通过在抗性动物和易感动物之间转移转基因,有几种方法可以做到 因此,包括转基因受体胚胎移植到假孕转基因供体,新生转基因交叉培养, 接受者到护理转基因捐赠者,简单地共同饲养动物,或实验性地施用制备的浆液, 从供体粪便到无菌或经驱虫处理的转基因受体(粪便微生物群转移,FMT)。是否 无论转基因是否成功,很少有研究仔细检查或报道转基因的效率。 从捐赠者到接受者的转移,很少有研究直接比较一种以上的转基因转移方法。 此外,已知供体和受体GM内微生物多样性的差异会影响 转基因成功。因此,应用研究第2部(具体目标1)的长远目标如下: 比较转基因转移的效率,使用上述方法和良好的控制和彻底 特征的转基因接受者和捐助者窝藏低和高多样性的转基因概况。具体目标1是测试 利用胚胎移植、交叉培养、共同饲养和FMT进行转基因移植的效率, 多样化程度低的全球机制捐助方和多样化程度高的接受方之间的资金转移,反之亦然,并确定 转基因转移的程度是否与表型转移的程度相关。 此外,虽然粪便代表允许个体纵向取样的非侵入性样品,但是, 对于小鼠,众所周知,粪便仅反映下胃肠道(GIT)。许多生理 过程和疾病机制依赖于存在于上GIT的GM,需要多个 用于纵向研究的小鼠终末队列,因为上GIT的非侵入性生前取样不是 目前可能。有鉴于此,应用研究第2节(具体目标)的长期目标 2)是开发和验证一个非侵入性的上GIT采样系统,用于下游测序 方法.与生物材料工程师团队合作,Specific Aim 2开发聚合物/金属 基于颗粒的系统,其能够在口服管饲后从上GIT选择性地收集细菌细胞, 然后从粪便中回收并分离用于下游分子分析。的发展 这样一个系统将通过减少所需的小鼠数量来推进比较医学的三个R 用于检查上消化道的纵向研究此外,这些方法将彻底改变人类 药物,并将立即获得专利,并可能导致随后的资金,通过多个 机制等总的来说,应用研究的目标第2节将大大提高能力, 来自广泛领域的研究人员以知情的方式操纵实验室小鼠的转基因, 以前无法访问的GIT样本区域。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Aaron Ericsson其他文献

Aaron Ericsson的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Aaron Ericsson', 18)}}的其他基金

The influence of complex gut microbiota on central nervous system development and adult cognition and behavior
复杂的肠道微生物群对中枢神经系统发育以及成人认知和行为的影响
  • 批准号:
    10063589
  • 财政年份:
    2019
  • 资助金额:
    $ 4.6万
  • 项目类别:
Impact of gut microbiota and segmented filamentous bacteria on colitis-associated colorectal cancer
肠道微生物群和分段丝状细菌对结肠炎相关结直肠癌的影响
  • 批准号:
    9908196
  • 财政年份:
    2016
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 1
密苏里大学突变小鼠资源和研究中心 - 应用研究第 1 部分
  • 批准号:
    10214837
  • 财政年份:
    2000
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 1
密苏里大学突变小鼠资源和研究中心 - 应用研究第 1 部分
  • 批准号:
    10360485
  • 财政年份:
    2000
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 2
密苏里大学突变小鼠资源和研究中心 - 应用研究第 2 部分
  • 批准号:
    10578802
  • 财政年份:
    2000
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 1
密苏里大学突变小鼠资源和研究中心 - 应用研究第 1 部分
  • 批准号:
    10578796
  • 财政年份:
    2000
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 2
密苏里大学突变小鼠资源和研究中心 - 应用研究第 2 部分
  • 批准号:
    9923244
  • 财政年份:
  • 资助金额:
    $ 4.6万
  • 项目类别:
The Mutant Mouse Resource and Research Center at the University of Missouri - Applied Research Section 1
密苏里大学突变小鼠资源和研究中心 - 应用研究第 1 部分
  • 批准号:
    9923243
  • 财政年份:
  • 资助金额:
    $ 4.6万
  • 项目类别:

相似海外基金

The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
  • 批准号:
    EP/Z000920/1
  • 财政年份:
    2025
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
  • 批准号:
    FT230100276
  • 财政年份:
    2024
  • 资助金额:
    $ 4.6万
  • 项目类别:
    ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
  • 批准号:
    MR/X024261/1
  • 财政年份:
    2024
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
  • 批准号:
    DE240100388
  • 财政年份:
    2024
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Discovery Early Career Researcher Award
Zootropolis: Multi-species archaeological, ecological and historical approaches to animals in Medieval urban Scotland
Zootropolis:苏格兰中世纪城市动物的多物种考古、生态和历史方法
  • 批准号:
    2889694
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Studentship
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
  • 批准号:
    2842926
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
  • 批准号:
    NC/X001644/1
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Training Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
  • 批准号:
    2337595
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Continuing Grant
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
  • 批准号:
    2232190
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
  • 批准号:
    23K17514
  • 财政年份:
    2023
  • 资助金额:
    $ 4.6万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了