Circadian regulation of neocortex

新皮质的昼夜节律调节

• 批准号: 10365299
• 负责人: Erik Herzog
• 金额: $ 41.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-12-01 至 2026-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10365299
• 关键词: Ablation; Alzheimer' s Disease; Area; Astrocytes; Behavior; Behavioral; Brain; Brain region; Calcium; Cells; Circadian Rhythms; Color; Corticosterone; Data; Disease; Eating; Food; Foundations; Gene Expression; Genes; Glucocorticoid Receptor; Glucocorticoids; Grooming; Hormone secretion; Hypothalamic structure; In Vitro; Light; Link; Literature; Liver; Locomotion; Machine Learning; Mammals; Mate Selections; Methods; Modeling; Motor; Motor Activity; Motor Cortex; Mus; Neocortex; Neuroglia; Neurons; Output; Pacemakers; Pancreas; Pattern; Periodicity; Peripheral; Physiology; Prevalence; Property; Regulation; Research Personnel; Rest; Retina; Role; Schedule; Schizophrenia; Signal Transduction; Skeletal Muscle; Sleep; Smell Perception; System; Techniques; Testing; Time; Tissues; base; cell type; circadian; circadian biology; circadian pacemaker; circadian regulation; drinking; experimental study; hippocampal pyramidal neuron; in vivo; learning ability; motor behavior; olfactory bulb; real-time images; receptor expression; suprachiasmatic nucleus; tool

Project Summary Little is known about the mechanisms that determine daily rhythms in rest-activity. We recently found that cells within the motor cortex can be synchronized to daily cycles of glucocorticoids. We hypothesize that daily suprachiasmatic nucleus activity and corticosterone secretion entrain circadian rhythms in cortical neurons and astrocytes to regulate daily patterns of behavior. We will take advantage of new high-throughput, machine learning and the ability to record and manipulate gene expression and calcium levels in specific cell types in mice. Combining these methods with two-color, real-time imaging of gene expression simultaneously in neurons and astrocytes, we will determine the roles of cortical neurons and glial in distinct daily activities.

项目摘要 人们对决定休息-活动的每日节律的机制知之甚少。我们最近 发现运动皮层内的细胞可以与糖皮质激素的每日周期同步。 我们假设视交叉上核的日常活动和皮质酮的分泌 皮质神经元和星形胶质细胞的昼夜节律来调节日常行为模式。我们 将利用新的高通量，机器学习和记录能力， 操纵小鼠特定细胞类型中的基因表达和钙水平。组合这些 方法用双色，实时成像的基因表达，同时在神经元和 星形胶质细胞，我们将确定皮质神经元和神经胶质细胞在不同的日常活动中的作用。