Exploring the Ecological Role of Rothia mucilaginosa and It's Iron Binding Siderophore Enterobactin

探索 Rothia mucilaginosa 及其铁结合铁载体肠杆菌素的生态作用

基本信息

  • 批准号:
    10366025
  • 负责人:
  • 金额:
    $ 29.25万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-03-05 至 2024-02-29
  • 项目状态:
    已结题

项目摘要

Project Summary The oral cavity is a highly diverse microbial environment, consisting of >2000 bacterial, archaeal, and fungal species most of which have not been functionally characterized. Numerous studies have identified that oral Rothia mucilaginosa, a Gram-positive oral commensal Actinobacteria, is highly abundant in saliva and dental plaque in global human populations, however, its ecological role is unknown. We identified that R. mucilaginosa produced the catechol siderophore enterobactin, the strongest iron-chelating molecule known. We also identified the enterobactin biosynthetic gene cluster (ent-BGC) in global Rothia genomes, which suggests that enterobactin is crucial in Rothia ecology. The purified enterobactin compound impacted growth differentially when amended to cultures of other oral bacterial species. It boosted the growth of commensal Streptococcus salivarius while it reduced the growth of some strains of pathogenic S. mutans. The overarching goal of this study is to determine the role of R. mucilaginosa produced enterobactin in interactions with both the oral microbiota and human oral epithelial cells representing the oral mucosa. We propose an interdisciplinary research approach with two specific aims: Aim 1: Characterization of molecular and ecological responses of oral bacteria to R. mucilaginosa produced enterobactin. The activities of enterobactin will be characterized both in highly diverse oral in vitro grown biofilms, and in bacterial monocultures. The capacity to import enterobactin by different bacterial community members will be revealed by amending growth cultures with fluorescently rhodamine- labeled enterobactin, fluorescence-activated cell sorting, and confocal microscopy. Multi-OMICS sequencing will be conducted to characterize functional changes both in biofilm communities as well as in single and dual- species cultures, specifically targeting genes encoding transport proteins and release mechanisms of enterobactin. Aim 2: Determine the impact of enterobactin on oral mucosal sentinel cells. Co-cultivation systems including both 2D and 3D models of human oral epithelial cells will be applied to study interactions with the oral microbiota and enterobactin. Outcomes of the interactions will be characterized by using a multipronged approach including a comparative gene transcription approach and a gene reporter system that reveals ROS activation in host cells. Production of extracellular pro- and anti-inflammatory cytokines in cell medium relation to enterobactin and oral bacteria will also be addressed to elucidate interactions of importance. The proposed study provides a unique opportunity to expand our knowledge on enterobactin functional role in the oral microbiota, and in interactions with human oral epithelial cells, which is severely lacking. A deeper knowledge of the role of iron scavenging siderophores in the oral cavity will likely bring about a shift in the research field of oral microbial ecology and shine a new light on the importance of iron metabolism in oral health.
项目摘要 口腔是一个高度多样化的微生物环境,包括>2000细菌,古细菌和真菌 大多数物种的功能尚未确定。许多研究表明,口服 粘液罗氏菌(Rothia mucilaginosa)是一种革兰氏阳性的口腔粘膜放线菌, 然而,全球人口中的斑块,其生态作用是未知的。我们发现,R。mucilaginosa 产生了邻苯二酚铁载体肠杆菌素,已知最强的铁螯合分子。我们还确定 全球罗氏菌基因组中肠杆菌素生物合成基因簇(ent-BGC),这表明肠杆菌素 在罗西亚生态学中至关重要纯化的肠杆菌素化合物影响生长差异时, 其他口腔细菌的培养物。它促进了唾液链球菌的生长, 抑制了某些致病性S.变异人本研究的总体目标是确定 R.粘液菌在与口腔微生物群和人类口腔微生物群的相互作用中产生肠杆菌素。 代表口腔粘膜的上皮细胞。我们提出了一个跨学科的研究方法与两个 具体目的:目的1:口腔细菌对R. 粘液菌产生肠杆菌素。肠杆菌素的活性将以高度多样性 口腔体外生长的生物膜和细菌单一培养物。不同国家进口肠杆菌素的能力 细菌群落成员将通过用荧光罗丹明修正生长培养物来揭示, 标记的肠杆菌素、荧光激活细胞分选和共聚焦显微镜。多OMICS测序将 进行表征生物膜群落以及单和双- 种培养物,特异性靶向编码转运蛋白的基因和 肠杆菌素目的2:确定肠杆菌素对口腔粘膜哨兵细胞的影响。共培养 包括人类口腔上皮细胞的2D和3D模型的系统将用于研究与 口腔菌群和肠杆菌素。互动的结果将通过使用多管齐下的 包括比较基因转录方法和揭示ROS的基因报告系统的方法 激活宿主细胞。细胞外促炎和抗炎细胞因子的产生与细胞介质的关系 肠杆菌素和口腔细菌也将解决阐明相互作用的重要性。 这项拟议的研究提供了一个独特的机会来扩大我们对肠杆菌素功能作用的了解, 口腔微生物群,以及与人类口腔上皮细胞的相互作用,这是严重缺乏的。更深 了解口腔中铁清除铁载体的作用可能会改变口腔中铁的吸收, 口腔微生物生态学的研究领域,并照耀在口腔健康的铁代谢的重要性新的光。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Piloting a city health adaptation typology with data from climate-engaged cities: Toward identification of an urban health adaptation gap.
利用气候参与城市的数据试点城市健康适应类型:确定城市健康适应差距。
  • DOI:
    10.1016/j.envres.2020.110435
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    8.3
  • 作者:
    Sheehan,MaryC;Freire,Mila;Martinez,GerardoSanchez
  • 通讯作者:
    Martinez,GerardoSanchez
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Marcelo Freire其他文献

Marcelo Freire的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Marcelo Freire', 18)}}的其他基金

Exploring the Ecological Role of Rothia mucilaginosa and It's Iron Binding Siderophore Enterobactin
探索 Rothia mucilaginosa 及其铁结合铁载体肠杆菌素的生态作用
  • 批准号:
    10218502
  • 财政年份:
    2021
  • 资助金额:
    $ 29.25万
  • 项目类别:
Regulation of ChemR23 in Resolution of Inflammation
ChemR23 在炎症消退中的调节
  • 批准号:
    9750754
  • 财政年份:
    2018
  • 资助金额:
    $ 29.25万
  • 项目类别:
Regulation of ChemR23 in Resolution of Inflammation
ChemR23 在炎症消退中的调节
  • 批准号:
    8568302
  • 财政年份:
    2013
  • 资助金额:
    $ 29.25万
  • 项目类别:
Regulation of ChemR23 in Resolution of Inflammation
ChemR23 在炎症消退中的调节
  • 批准号:
    9076616
  • 财政年份:
    2013
  • 资助金额:
    $ 29.25万
  • 项目类别:
Regulation of ChemR23 in Resolution of Inflammation
ChemR23 在炎症消退中的调节
  • 批准号:
    8669965
  • 财政年份:
    2013
  • 资助金额:
    $ 29.25万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了