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Microfluidic platform for solid tumor mechanics and invasion

用于实体瘤力学和侵袭的微流控平台

基本信息

批准号：
10366750
负责人：
Mingming Wu
金额：
$ 38.48万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10366750
关键词：
3-Dimensional Architecture Atomic Force Microscopy Behavior Biocompatible Materials Biological Assay Biological Sciences Blood Vessels Cancer Prognosis Cell Adhesion Molecules Cell Culture Techniques Cell Line Cell-Cell Adhesion Cells Cessation of life Clinical Data Analyses Distant Drug Delivery Systems E-Cadherin Engineering Equus caballus Extracellular Matrix Feedback Funding Generations Goals Image Impairment In Vitro Invaded Knowledge Lead Malignant - descriptor Malignant Neoplasms Masks Materials Testing Measures Mechanics Membrane Methods Microfluidics Molecular Neoplasm Metastasis Optics Organ Organoids Pathway interactions Physiological Play Primary Neoplasm Prognosis Reporter Research Role Running Secondary to Site Solid Solid Neoplasm Stress Stretching Techniques Technology Testing Theoretical model Time Tissue Engineering Tissues Tumor Cell Invasion Tumor Cell Migration Work cell type computer program diagnostic strategy diagnostic tool engineered stem cells experimental study force sensor in vivo innovation interstitial intravital imaging microscopic imaging molecular dynamics neoplastic cell novel diagnostics optical imaging pressure rapid growth response tool treatment strategy tumor tumor progression vascular abnormality

项目摘要

Project Summary Cancer metastasis accounts for over 90% of all cancer deaths. Important abilities of metastatic tumor cells include breaking away from the primary tumor and invading into surrounding tissue before disseminating to secondary tumor sites. Solid tumor stress caused by rapid growth of tumor cells and abnormality of the vascular tissue has long been associated with poor prognosis of cancer. Despite the clinical importance, the basic understanding of tumor mechanics and its relation to tumor invasion is lacking. This is in part due to the lack of in vitro tools that are able to investigate quantitatively tumor mechanics in a physiologically relevant 3D setting. Current material mechanical testing tools such as the rheometer have played important roles in our current understanding of biomaterials and tumor mechanics. However, the conventional rheometer is not easily made compatible with cell culture conditions and results are spatially averaged, masking important single cell and molecular level information. Atomic force microscopy and pipette aspiration are cell culture compatible, but low throughput. The goal of the proposed research is to develop a high throughput microfluidic rheometer for systematic studies of tumor mechanics and invasion in a physiologically realistic 3D setting and compatible with dynamic optical imaging at single cell and spheroid levels. We will deliver a set of principles that govern tumor mechanics and its relation with invasion. We postulate that tumor mechanics is a key predictor for tumor invasiveness. The proposed project is innovative because it represents the first generation of microfluidic rheometers that are capable of full mechanical testing for tumor mechanics studies, and at the same time compatible with tumor invasion experiments. Tools developed here can be easily extended to use for other living materials, and lessons learned here will eventually lead to knowledge important for developing novel diagnostic or/and treatment strategies for cancer.

项目总结