Consolidated Biomedical Core Facilities Supporting a Center on Emerging and Zoonotic Infectious Disease Research

支持新发和人畜共患传染病研究中心的综合生物医学核心设施

• 批准号: 10372482
• 负责人: Bonnie Rush
• 金额: $ 343.1万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-20 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10372482
• 关键词: Animal Diseases; Animal Model; Biomedical Research; CRISPR/Cas technology; Cell Separation; Cells; Centers of Research Excellence; Communicable Diseases; Complex; Computers and Advanced Instrumentation; Confocal Microscopy; Core Facility; DNA; Development; Discipline; Elements; Environment; Faculty; Floor; Flow Cytometry; Fostering; Image; Individual; Infectious Diseases Research; Infrastructure; Institution; Kansas; Laboratories; Location; Modernization; Molecular Analysis; Molecular and Cellular Biology; Nucleic Acids; Pathology; Phase; Positioning Attribute; Primary Health Care; Productivity; Research; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Research Training; Science; Services; Technology; Time; Tissues; Training; Training Support; Universities; Zoonoses; arthropod-borne; biodefense; biosafety level 4 facility; biosecurity; clinical care; experimental study; flexibility; in vivo imaging; laser capture microdissection; member; microscopic imaging; next generation sequencing; research facility; transcriptome sequencing

SUMMARY: Kansas State University (KSU) is well-positioned to become the preeminent institution to advance the discovery and development of biosecurity strategies for emerging and zoonotic infectious diseases. With KSU’s Biosecurity Research Institute (BSL-3), USDA’s Arthropod-Borne Animal Disease Research Unit, and USDA’s National Bio and Agro-Biodefense Facility (BSL-3/BSL-4), KSU is the only U.S. university with a full continuum of biosecurity level 1 (BSL-1) through BSL-4 facilities collocated on one campus. The proposed core research facility represents a critical component of our research infrastructure to support infectious disease studies and provide direct support of both KSU’s 2020 COBRE center and collocated federal facilities. The core facility will strategically combine five key disciplines: animal model/pathology, molecular and cellular biology, microscopic imaging, flow cytometry and cell sorting, and next-generation sequencing. Investigators at KSU and in the region rely on these technologies to perform high-impact research and complete experiments outlined in current and future research projects. However, existing facilities for these key technologies are inadequate in size, location, function, and flexibility. Existing laboratories are isolated from each other, spread across three buildings, and in some cases, are hosted by individual faculty members, which limits access and research productivity. The proposed core-facility suite is the final element of a three-phase renovation of the KSU veterinary complex. Phase 1 delivered a contemporary 220-seat auditorium (8,200 GSF) adjacent to Mosier Hall. Phase 2 replaced an outdated auditorium with a primary-care clinical training facility occupying the 1st floor of Mosier Hall. Phase 3, the focus of this application, proposes to build a collaborative, university-wide core research facility occupying the 2nd floor of the deconstructed auditorium space. Currently, the 2nd floor is 5,000 GSF of laboratory-conditioned shell space. This proposal will strengthen our research capacity and infrastructure by creating a centrally organized, integrated technology pipeline, with proximate expert assistance and training support to facilitate efficient use of contemporary technology in confocal microscopy, live-cell and in vivo imaging, laser capture microdissection, flow cytometry, cell sorting, DNA/RNA sequencing and CRISPR technology. The proposed purpose-built, core-facility suite will assemble state-of-the-art technologies in a single location to deliver efficient, coordinated services for academic, corporate and federal researchers in imaging and molecular analyses, thereby providing a complete range of services from whole tissues to single-cell nucleic acid analyses. A dedicated modern biomedical research facility with advanced instrumentation and technical support will foster collaborative, synergistic and transdisciplinary science. This is critical to promoting a robust research and training environment where researchers can answer the most challenging and urgent biomedical questions of our time.

摘要：堪萨斯州立大学 (KSU) 完全有能力成为推动经济发展的卓越机构 发现和制定针对新出现的人畜共患传染病的生物安全策略。和 KSU 生物安全研究所 (BSL-3)、美国农业部节肢动物传播动物疾病研究组，以及 美国农业部国家生物和农业生物防御设施 (BSL-3/BSL-4)，KSU 是唯一一所拥有完整设施的美国大学 生物安全 1 级 (BSL-1) 到 BSL-4 设施的连续统一体配置在一个校园内。拟议的核心 研究设施是我们支持传染病的研究基础设施的重要组成部分 研究并为 KSU 的 2020 COBRE 中心和并置的联邦设施提供直接支持。核心 该设施将战略性地结合五个关键学科：动物模型/病理学、分子和细胞生物学、 显微成像、流式细胞术和细胞分选以及下一代测序。 KSU 和 该地区依靠这些技术来进行高影响力的研究并完成中概述的实验 当前和未来的研究项目。然而，现有的这些关键技术的设施还不够充分。 尺寸、位置、功能和灵活性。现有实验室彼此隔离，分布在三个区域 建筑物，在某些情况下，由个别教职人员主持，这限制了访问和研究 生产率。拟议的核心设施套件是 KSU 三阶段改造的最后一个要素 兽医综合体。第一阶段在 Mosier Hall 附近建造了一个拥有 220 个座位的现代礼堂（8,200 GSF）。 第二阶段用占据一楼的初级保健临床培训设施取代了过时的礼堂 莫西尔大厅。第三阶段是本申请的重点，建议建立一个协作的、大学范围内的核心 研究设施占据解构礼堂空间的二楼。目前二楼有5000 实验室条件壳空间的 GSF。该提案将加强我们的研究能力和基础设施 通过创建集中组织的综合技术管道，并提供直接的专家协助和培训 支持促进共焦显微镜、活细胞和体内成像中当代技术的有效利用， 激光捕获显微切割、流式细胞术、细胞分选、DNA/RNA 测序和 CRISPR 技术。这 拟议的专用核心设施套件将在一个地点汇集最先进的技术， 为成像和分子领域的学术、企业和联邦研究人员提供高效、协调的服务 分析，从而提供从整个组织到单细胞核酸分析的全套服务。 拥有先进仪器和技术支持的专用现代生物医学研究设施将促进 协作、协同和跨学科科学。这对于促进强有力的研究和培训至关重要 研究人员可以回答我们这个时代最具挑战性和紧迫的生物医学问题的环境。