Swine Biomedical Research Facility for gnotobiotic, transgenic and translational medicine

该项目的总体目标是设计，建造和装备一个猪生物医学研究设施， 美国排名第四的兽医学院的校园， 训练有素的兽医专家和最先进的生物医学（如MRI、CT和核医学）设施。 该设施将提供额外的高质量的空间，由国家卫生研究院资助的教师从北卡罗来纳州的生物医学研究 州立大学、杜克大学和北卡罗来纳州大学。 猪生物医学研究设施的设计和建造将采用独立的 砖石和钢结构建筑，将用于生产和护理非细菌猪和基因编辑猪，例如 以及最先进的手术（外科手术、遥测、关节镜检查、内窥镜检查）。此外，该单位还包括 灵活的空间，可以容纳怀孕和非怀孕母猪，以及分娩设施，以产生 我们需要从我们自己的品系以及从NIH支持的NSRRC获得的基因编辑后代。 手术空间将提供一个无菌手术室（两张手术台），以适应不断增加的 内窥镜和关节镜手术的生物工程需求。这座建筑将立即被安置在 毗邻空间（称为G20设施）以前创建的使用严重的结合 免疫缺陷和其他基因编辑的小型猪或幼龄猪（G20 OD 020279），以允许共享使用 空间，如果可能的话。拟议的设施旨在最大限度地发挥协同作用， 与G20的空间重叠。结合起来，他们将给我们一个高度的灵活性，并将使我们能够进行 广泛的研究，从而对多个NIH中心/研究所产生广泛的影响。 该项目团队的独特之处在于推动该设施的设计和开发， 扩大这一方案。通过在兽医学院和NC担任领导职务， 我们有能力为州立大学提供进入兽医学院生物医学校区的机会， 研究动物设施和三级兽医医院的最先进设备。我们 研究团队拥有比较医学专业知识和广泛的合作网络， 杜克大学和北卡罗来纳州大学的生物医学研究人员。作为一个团队，我们成功地 管理基础设施赠款，如扩大住房和研究转基因和 非转基因小型猪（G20 OD 020279）和建设项目，包括一个20，000平方英尺的湿实验室 GMP实验室空间

The overall goal of this project is to design, construct and outfit a swine biomedical research facility on the campus of the fourth ranked College of Veterinary Medicine in the United States with ready access to trained veterinary specialists and state of the art biomedical (e.g. MRI, CT and nuclear medicine) facilities. The facility will provide additional high-quality space for biomedical research by NIH funded faculty from NC State University, Duke University and the University of North Carolina. The design and construction of the swine biomedical research facility will feature a free-standing masonry and steel building that will house the production and care of gnotobiotic and gene-edited swine, as well as state-of-the-art procedures (surgical, telemetry, arthroscopy, endoscopy). In addition, the unit includes flexible space that can accommodate pregnant and non-pregnant sows, and farrowing facilities to generate needed gene edited progeny from our own lines as well as those obtained from the NIH-supported NSRRC. Procedural space will provide a sterile surgery suite (two tables) to accommodate an increasing bioengineering need for endoscopic and arthroscopic procedures. The building will be placed immediately adjacent to space (referred to as the G20 facility) previously created for the use of severe combined immunodeficiency and other gene edited miniature or juvenile pigs (G20 OD020279) to allow for shared use of the space when possible. The proposed facility has been designed to maximize synergy and minimize overlap with the G20 space. Combined they will give us a high degree of flexibility and will allow us to conduct a broad range of research thus having a broad impact across multiple NIH Centers/Institutes. This project team is uniquely situated to drive the design and development of this facility and the expansion of this program. By serving in leadership roles within the College of Veterinary Medicine and NC State University we have the ability to provide access to the veterinary college biomedical campus, the research animal facilities and the state-of-the-art equipment in the tertiary care veterinary hospital. Our team of investigators has comparative medicine expertise and an extensive collaborative network with biomedical researchers at Duke and the University of North Carolina. As a team, we have successfully managed infrastructure grants, such as the expansion of facilities for housing and studying transgenic and non-transgenic miniature pigs (G20 OD020279) and building projects including a 20,000 square foot wet lab and GMP lab space.