A Targeted DNA Adductomics Approach for Analyzing > 100 DNA Adducts

用于分析 > 100 个 DNA 加合物的靶向 DNA 加合物组学方法

基本信息

  • 批准号:
    10371133
  • 负责人:
  • 金额:
    $ 34.99万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-06-15 至 2024-03-31
  • 项目状态:
    已结题

项目摘要

ABSTRACT Endogenous metabolism and environmental exposure can give rise to DNA adduct formation. Robust measurement of the levels of DNA adducts can offer important knowledge for understanding the mechanisms of DNA repair and allow for assessment of human exposure to genotoxic chemicals. We reason that a scheduled selected-reaction monitoring (SRM) method for DNA adduct analysis will facilitate high-throughput analysis of the DNA adductome. The long-term objective of this application is to develop a targeted DNA adductomic assay for assessing quantitatively the DNA adducts induced by various endogenous and exogenous sources of DNA damaging agents, and to employ the method for monitoring human exposure to complicated environmental pollutants and for investigating DNA repair. The proposed research is organized into three Specific Aims. In Aim #1, we will establish an SRM library for targeted quantitative measurement of the DNA adductome. In Aim 2, we will profile comprehensively the DNA adductome in lung tissues of mice exposed to particulate matter air pollution. In Aim 3, we will assess the oxidative metabolism and repair of alkylated DNA lesions by Fe2+- and 2- oxoglutarate-dependent oxygenases. The outcome of the proposed research will provide the scientific community with a high-throughput platform that allows for monitoring the DNA adductome and will afford novel insights about the repair of alkylated DNA lesions.
摘要

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
HILIC-MS/MS for the Determination of Methylated Adenine Nucleosides in Human Urine.
  • DOI:
    10.1021/acs.analchem.1c03829
  • 发表时间:
    2021-12-28
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Guo C;Hu Y;Cao X;Wang Y
  • 通讯作者:
    Wang Y
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Yinsheng Wang其他文献

Yinsheng Wang的其他文献

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{{ truncateString('Yinsheng Wang', 18)}}的其他基金

Chemical Biology of DNA and RNA Alkylation
DNA 和 RNA 烷基化的化学生物学
  • 批准号:
    10597056
  • 财政年份:
    2020
  • 资助金额:
    $ 34.99万
  • 项目类别:
Chemical Biology of DNA and RNA Alkylation
DNA 和 RNA 烷基化的化学生物学
  • 批准号:
    10376803
  • 财政年份:
    2020
  • 资助金额:
    $ 34.99万
  • 项目类别:
Chemical Biology of DNA and RNA Alkylation
DNA 和 RNA 烷基化的化学生物学
  • 批准号:
    10190950
  • 财政年份:
    2020
  • 资助金额:
    $ 34.99万
  • 项目类别:
Chemistry and Biology of Alkyl Phosphotriester Lesions
烷基磷酸三酯损伤的化学和生物学
  • 批准号:
    10520048
  • 财政年份:
    2019
  • 资助金额:
    $ 34.99万
  • 项目类别:
Chemistry and Biology of Alkyl Phosphotriester Lesions
烷基磷酸三酯损伤的化学和生物学
  • 批准号:
    9896297
  • 财政年份:
    2019
  • 资助金额:
    $ 34.99万
  • 项目类别:
Chemistry and Biology of Alkyl Phosphotriester Lesions
烷基磷酸三酯损伤的化学和生物学
  • 批准号:
    10307544
  • 财政年份:
    2019
  • 资助金额:
    $ 34.99万
  • 项目类别:
A Targeted DNA Adductomics Approach for Analyzing > 100 DNA Adducts
用于分析 > 100 个 DNA 加合物的靶向 DNA 加合物组学方法
  • 批准号:
    9883797
  • 财政年份:
    2018
  • 资助金额:
    $ 34.99万
  • 项目类别:
Quantitative Adductomics Approaches for Assessing the Occurrence and Repair of DNA Adducts
用于评估 DNA 加合物的发生和修复的定量加合物组学方法
  • 批准号:
    10172860
  • 财政年份:
    2017
  • 资助金额:
    $ 34.99万
  • 项目类别:
Quantitative Adductomics Approaches for Assessing the Occurrence and Repair of DNA Adducts
用于评估 DNA 加合物的发生和修复的定量加合物组学方法
  • 批准号:
    9389996
  • 财政年份:
    2017
  • 资助金额:
    $ 34.99万
  • 项目类别:
Enzymatic Conversions of Tet-mediated Oxidation Products of 5-Methylcytosine
Tet 介导的 5-甲基胞嘧啶氧化产物的酶促转化
  • 批准号:
    8987741
  • 财政年份:
    2015
  • 资助金额:
    $ 34.99万
  • 项目类别:

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