An LTQ-Orbitrap Elite with ETD for the Keck Proteomics Facility

描述(由申请人提供)：申请资金用于购买具有电子转移解离(ETD)能力的Thermo LTQ-Orbitrap Elite质谱仪。所要求的仪器将安装在加州大学河滨分校的W.M.Keck蛋白质组学设施并由其管理。加州大学河滨分校有一个由NIH资助的14名研究人员组成的核心小组，他们需要广泛使用拟议的LTQ-Orbitrap Elite系统进行蛋白质组分析。目前，由于缺乏用于全球蛋白质组定量、翻译后修饰、蛋白质复合体和蛋白质构象研究的大规模图谱的高性能LC-MS/MS系统，这些研究项目的进展受到阻碍。拟议文书的问世将对这些研究项目的进展产生直接和积极的影响。特别是，线性离子陷阱的高扫描速度，加上Orbitrap质谱仪的高质量分辨能力和高质量精度，提供了以高置信度识别和定量大量蛋白质的能力。此外，电子转移解离作为一种离子解离技术在所建议的仪器上的可用性有助于以极高的精度明确地识别翻译后修饰。这一最先进的蛋白质组平台的推出将进一步促进加州大学河滨分校的生物医学研究，并增强来自代表不足的少数群体的学生和博士后的研究体验。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Thermo LTQ-Orbitrap Elite mass spectrometer with electron transfer dissociation (ETD) capability. The requested instrument will be installed at, and managed by the W. M. Keck Proteomics Facility at the University of California Riverside. There are a core group of 14 NIH-funded investigators at UC Riverside who require extensive access to the proposed LTQ-Orbitrap Elite system for proteomic analysis. The progress of these research projects is currently hampered by the lack of a high- performance LC-MS/MS system for global proteome quantification, large-scale mapping of post-translational modifications, protein complex, and protein conformation studies. The availability of the proposed instrument will have an immediate and positive impact on the progress of these research projects. In particular, the high scan rate of the linear ion trap, together with the high mass-resolving power and high mass accuracy of the Orbitrap mass analyzer, provides capability in identifying and quantifying a large number of proteins with high confidence. In addition, the availability of electron transfer dissociation as an ion dissociation technique on the proposed instrument facilitates the unambiguous identifications of post-translational modifications with great accuracy. The availability of this state-of-the-art proteomis platform will further promote biomedical research at UC Riverside and enhance the research experience of students and post-docs from under-represented minority groups.