An LTQ-Orbitrap Elite with ETD for the Keck Proteomics Facility

用于 Keck 蛋白质组学设施的带 ETD 的 LTQ-Orbitrap Elite

• 批准号: 8447946
• 负责人: Yinsheng Wang
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447946
• 关键词: Biomedical Research; California; Dissociation; Electron Transport; Funding; Funding Applicant; Ions; Maps; Minority Groups; Performance; Post-Translational Protein Processing; Postdoctoral Fellow; Protein Conformation; Proteins; Proteome; Proteomics; Research; Research Personnel; Research Project Grants; Scanning; Students; System; Techniques; Underrepresented Minority; United States National Institutes of Health; Universities; experience; instrument; mass analyzer; mass spectrometer; protein complex

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Thermo LTQ-Orbitrap Elite mass spectrometer with electron transfer dissociation (ETD) capability. The requested instrument will be installed at, and managed by the W. M. Keck Proteomics Facility at the University of California Riverside. There are a core group of 14 NIH-funded investigators at UC Riverside who require extensive access to the proposed LTQ-Orbitrap Elite system for proteomic analysis. The progress of these research projects is currently hampered by the lack of a high- performance LC-MS/MS system for global proteome quantification, large-scale mapping of post-translational modifications, protein complex, and protein conformation studies. The availability of the proposed instrument will have an immediate and positive impact on the progress of these research projects. In particular, the high scan rate of the linear ion trap, together with the high mass-resolving power and high mass accuracy of the Orbitrap mass analyzer, provides capability in identifying and quantifying a large number of proteins with high confidence. In addition, the availability of electron transfer dissociation as an ion dissociation technique on the proposed instrument facilitates the unambiguous identifications of post-translational modifications with great accuracy. The availability of this state-of-the-art proteomis platform will further promote biomedical research at UC Riverside and enhance the research experience of students and post-docs from under-represented minority groups.

说明（申请人提供）：申请资金用于购买具有电子转移解离（ETD）能力的Thermo LTQ-Orbitrap Elite质谱仪。所要求的仪器将安装在W. M.加州滨江大学的凯克蛋白质组学研究中心。加州大学滨江有一个由14名NIH资助的研究人员组成的核心小组，他们需要广泛使用拟议的LTQ-Orbitrap Elite系统进行蛋白质组学分析。这些研究项目的进展目前受到缺乏用于全局蛋白质组定量、大规模翻译后修饰作图、蛋白质复合物和蛋白质构象研究的高性能LC-MS/MS系统的阻碍。拟议的仪器的可用性将对这些研究项目的进展产生直接和积极的影响。特别是，线性离子阱的高扫描速率，以及Orbitrap质量分析仪的高质量分辨能力和高质量准确度，提供了以高置信度鉴定和定量大量蛋白质的能力。此外，电子转移解离作为离子解离技术的建议仪器上的可用性有利于明确的翻译后修饰的识别具有很大的准确性。这个最先进的蛋白质组平台的可用性将进一步促进加州大学滨江分校的生物医学研究，并增强来自代表性不足的少数群体的学生和博士后的研究经验。