Lipidomics Shared Resource

脂肪组学共享资源：综述 脂肪组学共享资源(LSR)的使命是提供具有成本效益的先进方法， 最先进的仪器和合成和分析生物活性脂类的专业知识可以- CER中心(HCC)成员。LSR的科学目标是为研究脂类代谢提供资源 和癌症中的信号，用于识别生物活性脂类及其在肿瘤细胞生物学中的作用，以及发现 以及评估针对脂质代谢过程的药物。通过提供深度探索性和高效性 分析癌细胞和组织的吞吐量脂肪组学选择，结合教育研讨会， LSR促进了肝细胞癌基础、临床和人群科学家之间的互动。保持领先地位 在一个不断发展的领域，自上次审查以来，LSR通过投资新设备扩大了服务(Thermo TSQ-50003、SCIEX Q-Trap三重四极/离子陷阱、Advion Triversa NANOMAT)和传输设备 从细胞与分子成像共享资源到LSR，为LSR增加MALDI成像服务(Bruker Faltonics Autoflex III线性MALDI和AutoFlexIII MALDI TOF-TOF)。在当前周期中，LSR di- 直接资助了45份癌症相关出版物，3个团队科学项目，多个内部资助的团队- 基础项目、三项癌症个人拨款、两项转化性研究和一项1期临床试验。在……下面 在贝西姆·奥格雷特曼博士的领导下，LSR已经成长为一个全国性和国际性的组织-- 为生物活性脂类分子的测量和分析提供新的复杂方法，使科学资源现代化。 与癌症的发生和发展相关的分子。奥格雷特曼是脂类生物学领域的全国领先者，拥有一种- 广泛关注鞘糖脂，包括鞘糖脂。LSR集成了MALDI成像功能，可用于 组织中鞘磷脂种类的直接组织分析及药物在移植瘤组织和器官中的分布 (脑、肾、肝)，并通过碰撞诱导碎片确认感兴趣的药物或代谢物的结构- 提顿。因此，通过将质谱学和MALDI成像相结合，该资源不仅可以量化鞘蛋白- 此外，还可以提供有关其在组织中的细胞位置和分布的信息。这个 LSR已做好充分准备，将推动和支持癌症研究中的重要新兴科学学科 成员和更广泛的科学界。

LIPIDOMICS SHARED RESOURCE: SUMMARY The mission of the Lipidomics Shared Resource (LSR) is to provide cost-effective advanced methodology, state-of-the-art instrumentation, and expertise in the synthesis and analysis of bioactive lipids to Hollings Can- cer Center (HCC) members. The scientific goal of the LSR is to provide resources for studying lipid metabolism and signaling in cancer, for identifying bioactive lipids and their role in tumor cell biology, and for discovering and evaluating drugs that target lipid metabolic processes. By providing deep exploratory as well as high throughput lipidomic options to analyze cancer cells and tissues, combined with educational workshops, the LSR fosters interactions among basic, clinical and population scientists at HCC. To remain at the leading edge of an evolving field, since the last review, the LSR expanded services by investing in new equipment (Thermo TSQ-50003, SCIEX Q-Trap triple quadrupole/ion trap, Advion TriVersa Nanomat) and transferring equipment from the Cell & Molecular Imaging Shared Resource to LSR, adding MALDI imaging services to LSR (Bruker Faltonics AutoFlex III linear MALDI, and AutoFLexIII MALDI TOF-TOF). During the current cycle, the LSR di- rectly supported 45 cancer-related publications, three team science projects, multiple internally funded team- based projects, three individual cancer grants, two translational studies, and one phase 1 clinical trial. Under the leadership of Besim Ogretmen, PhD, the LSR has grown to become a nationally- and internationally-recog- nized scientific resource with novel sophisticated methods for measurement and analysis of bioactive lipid mol- ecules relevant to cancer initiation and progression. Ogretmen is a national leader in lipid biology with a partic- ular focus on sphingolipids, including glycosphingolipids. The LSR has integrated MALDI imaging capability for direct tissue analysis of sphingolipid species in tissues, drug distribution in tumor xenograft tissue and organs (brain, kidney, liver), and structure confirmation of drug or metabolite of interest by collision-induced fragmenta- tion. Thus, by combining mass spectrometry and MALDI imaging, this resource not only quantifies sphin- golipids in tissues, but can also provide information about their cellular location and distribution in tissues. The LSR is well poised to advance and support important emerging scientific disciplines in cancer research to HCC members and the broader scientific community.