Molecular & Cellular Morphology Core

分子

• 批准号: 10377544
• 负责人: Milton j Finegold
• 金额: $ 22.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10377544
• 关键词: 3-Dimensional; Animal Diseases; Antibodies; Biological Assay; Cell Culture Techniques; Cells; Cellular Morphology; Cellular biology; Collection; Color; Computer software; Consultations; DNA Probes; Data; Data Analyses; Defect; Detection; Digestive System Disorders; Dimensions; Disease; Dissection; Educational workshop; Enzymes; Equipment; Exocrine pancreas; Experimental Designs; Fluorescence; Freezing; Frozen Sections; Funding; Gastrointestinal tract structure; Gene Expression Profiling; Generations; Health; Healthcare; Hepatic; Histocytochemistry; Histology; Hyperbaric Oxygen; Image; Image Analysis; Imaging Techniques; Immunoelectron Microscopy; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Individual; Infection; Injury; Institution; Instruction; Investigation; Laboratories; Lasers; Liver; Medical center; Messenger RNA; Methods; Microscope; Microscopy; Modeling; Molecular; Molecular Biology; Molecular Genetics; Morphology; Organelles; Organoids; Pathologic; Pathology; Pattern; Phase; Phenotype; Physiology; Play; Procedures; Productivity; Publications; RNA; Reader; Reproducibility; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Scanning; Scientist; Services; Slide; Specific qualifier value; Stains; Techniques; Testing; Texas; Tissues; Training; Virus Diseases; Work; animal tissue; base; biological research; cellular imaging; data acquisition; digital imaging; genetic analysis; high throughput screening; human tissue; imager; imaging informatics; innovation; instrument; instrumentation; interest; laboratory facility; luminescence; mRNA Expression; meetings; member; microscopic imaging; morphometry; mouse model; quantitative imaging; robot assistance; sample fixation; transmission process

Project Summary The Cellular and Molecular Morphology Core (Core B) of the Texas Medical Center (TMC) Digestive Disease Center (DDC) was founded in 2003, and it has played a key role in serving the needs of DDC members in the three institutions of the TMC DDC by providing expertise and specialized laboratory facilities. The Core provides a wide range of essential services of high utility via three sub-cores: Pathology (B1), Integrated Microscopy (B2) and RNA In Situ Hybridization (B3), each with a dedicated leader – Director with or without a Co-Director, staff and equipment; these sub-cores work together to serve DDC investigators by assisting scientists with experimental design, data acquisition and data interpretation. The major services provided by this Core include histology, immunohistochemistry, immunofluorescence, enzyme histochemistry, RNA in situ hybridization, confocal and deconvolution microscopy, super-resolution and high throughput microscopy, transmission, scanning, and immunoelectron microscopy, quantitative morphometry and high content single cell image analysis, laser capture dissection for molecular genetic analyses, and digital imaging for publications. The Core also provides consultation and training in the proper collection, fixation, storage and handling of human and animal tissues. A major benefit of the Core is utilization of information and expertise gained from diverse projects in individual laboratories to enhance the productivity of all. This is accomplished through workshops and meetings of the user groups at which new data, methods, and procedures are presented and their applicability to diverse investigations are demonstrated. This Core has been highly productive and beneficial for members of the TMC DDC, particularly to Pilot and Feasibility (P/F) awardees. During this funding cycle, 48 of the 59 Full Members used this Core. The requests, billing and tracking of services the Core are exclusively handled via the DDC implemented iLab Solutions software. The new or enhanced Core services include triple in situ hybridization, super-resolution microscopy, high throughput microscopy and image analysis. The first accessible super-resolution instrument, the GE Healthcare OMX BLAZE, will be made available to DDC members. The Biotek Cytation 5, a "microscope-in-a-box," is both a fully functional plate reader (absorbance, fluorescence and luminescence) and an advanced imager with four-color fluorescence, phase contrast, color brightfield, slide scanning, live capabilities, hyperbaric oxygen, automated scanning with basic image analysis and long distance objectives for imaging through cell culture vessels. For example, the addition of super-resolution microscopy techniques will allow DDC researchers to perform structural studies of viral infection, both in fixed and live samples. A new image analysis service is being used for 2D- 3D co-localization studies, and IHC quantitation. Subcore B1 has also titrated more than 150 antibodies for immunofolrescence assays.

项目概要 德克萨斯医学中心 (TMC) 消化系统的细胞和分子形态学核心（核心 B） 疾病中心（DDC）成立于2003年，在满足DDC的需求方面发挥了关键作用 TMC DDC 三个机构的成员提供专业知识和专业实验室 设施。该核心通过三个子核心提供广泛的高实用性基本服务： 病理学 (B1)、集成显微镜 (B2) 和 RNA 原位杂交 (B3)，每个都有专门的领导者 – 董事（有或没有联合董事）、人员和设备；这些子核协同工作为DDC服务 研究人员通过协助科学家进行实验设计、数据采集和数据解释。这 该核心提供的主要服务包括组织学、免疫组织化学、免疫荧光、 酶组织化学、RNA 原位杂交、共焦和反卷积显微镜、超分辨率 和高通量显微镜、透射、扫描和免疫电子显微镜、定量 形态测定和高内涵单细胞图像分析、分子遗传学激光捕获解剖 分析和出版物的数字成像。核心还提供适当的咨询和培训 人类和动物组织的收集、固定、储存和处理。核心的一个主要好处是 利用从各个实验室的不同项目中获得的信息和专业知识来增强 所有人的生产力。这是通过用户组的研讨会和会议来完成的，在这些研讨会和会议上，新的 介绍了数据、方法和程序，并介绍了它们对各种调查的适用性 证明了。该核心对于 TMC DDC 的成员来说非常高效且有益， 特别是对于试点和可行性（P/F）获奖者。在此资助周期内，59 项全额资助中的 48 项 成员使用了这个核心。核心服务的请求、计费和跟踪均由核心独家处理 通过 DDC 实施 iLab Solutions 软件。新的或增强的核心服务包括三重服务 原位杂交、超分辨率显微镜、高通量显微镜和图像分析。第一个 DDC 将提供易于使用的超分辨率仪器 GE Healthcare OMX BLAZE 成员。 Biotek Cytation 5 是一款“盒装显微镜”，同时也是功能齐全的读板器 （吸光度、荧光和发光）和具有四色荧光、相位的先进成像仪 对比度、彩色明场、载玻片扫描、实时功能、高压氧、自动扫描 通过细胞培养容器成像的基本图像分析和长距离物镜。例如， 超分辨率显微镜技术的加入将使 DDC 研究人员能够进行结构分析 对固定样本和活样本中的病毒感染进行研究。一种新的图像分析服务正在用于 2D- 3D 共定位研究和 IHC 定量。 Subcore B1 还滴定了 150 多种抗体 免疫荧光测定。