13 Development of magnetometer immunoassay technology for the rapid and cost effective detection of endemic animal diseases and pathogens (zoonoses)

13 开发磁力计免疫测定技术，用于快速且经济有效地检测地方性动物疾病和病原体（人畜共患病）

• 批准号: BB/L011360/1
• 负责人: Janice Kiely
• 金额: $ 37.38万
• 依托单位: University of the West of England
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL011360%2F1
• 关键词: 13; Development; magnetometer; immunoassay; technology

International trading of food from animal sources is very intensive. Regulations decree that the production of animal products is free from zoonoses that can affect humans. Food producers wish to avoid the costs associated with other animal diseases. Regulatory authorities wish to reduce the cost of testing, especially visual inspection at slaughterhouses. Building on solid scientific and commercial research evidence resulting from a recently completed TSB GRD-funded project, this project now aims to take MagSer (i.e.a serology test for zoonoses and animal diseases) from Proof of Principle (PoP) to a fully developed instrument and suite of assays for screening animal diseases and zoonoses. The novelty and advantage of MagSer lies in the unique combination of immuno-magnetic capture of paramagnetic particles (beads) with highly sensitive magnetometer instrumentation to detect and quantify antibodies captured on the beads. The main innovations will be the a disposable measurement technology directly applicable to the measurement of antigen specific antibodies in pig's blood and the development of a rapid, low cost and simple device that can be used at point of test to screen animals for infections and check for efficacy of vaccination. Assay development work is planned at UWE's labs with instrument and consumable development and assay integration and validation being carried out by Clarity Biosolutions Ltd. (CBL).

动物源食品的国际贸易非常密集。条例规定，动物产品的生产没有可能影响人类的人畜共患病。食品生产商希望避免与其他动物疾病相关的成本。监管机构希望降低检测成本，特别是屠宰场的目视检查。建立在坚实的科学和商业研究的证据，从最近完成的TSB GRD资助的项目，该项目现在的目标是采取MagSer（即人畜共患病和动物疾病的血清学测试）从原理证明（PoP）到一个完全开发的仪器和检测套件，用于筛选动物疾病和人畜共患病。MagSer的新奇和优势在于顺磁性颗粒（珠）的免疫磁性捕获与高灵敏度磁力计仪器的独特组合，以检测和定量珠上捕获的抗体。主要创新将是直接适用于测量猪血液中抗原特异性抗体的一次性测量技术，以及开发一种快速，低成本和简单的设备，可用于检测点，以筛选动物感染和检查疫苗接种的有效性。测定开发工作计划在UWE的实验室进行，仪器和耗材开发以及测定集成和验证由Clarity Biosolutions Ltd.（CBL）进行。

项目成果

An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen.

• DOI: 10.3390/bios4030204
• 发表时间: 2014-09
• 期刊: Biosensors
• 作者: Barnett JM;Wraith P;Kiely J;Persad R;Hurley K;Hawkins P;Luxton R
• 通讯作者: Luxton R

Mathematical modelling of a magnetic immunoassay

磁免疫分析的数学模型

• DOI: 10.1093/imamat/hxx034
• 发表时间: 2017
• 期刊: IMA Journal of Applied Mathematics
• 影响因子: 1.2
• 作者: Roberts L