Maximizing Investigators' Research Award
最大限度地提高研究人员的研究奖
基本信息
- 批准号:10380617
- 负责人:
- 金额:$ 56.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-04-01 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:AreaAutophagocytosisAwardBiochemicalCellsEndoplasmic ReticulumGenomicsMammalian CellMicroscopyMitochondriaMolecularOrganellesProteinsQuality ControlResearchResearch PersonnelSaccharomyces cerevisiaeSaccharomycetalesTailTheoretical modelTimeWorkYeastsgenome wide screeninterestprotein aggregationreconstitution
项目摘要
ABSTRACT
My lab has two main areas of interest: 1) tail-anchored (TA) protein targeting and quality control;
2) selective autophagy. Given that selective autophagy targets toxic protein aggregates and
damaged organelles for destruction, these two research areas have strong conceptual ties. In
many cases we are answering similar questions about substrate selectivity: What distinguishes
newly-synthesized TA proteins as substrates for endoplasmic reticulum protein targeting from TA
proteins targeted to mitochondria? What distinguishes TA proteins that are mistargeted to
mitochondria as quality control substrates from native mitochondrial TA proteins? What
distinguishes apparently damaged organelles as selective autophagy substrates from healthy
organelles? To answer these questions, we are dissecting diverse molecular mechanisms that
select substrates of grossly different sizes and operate on very different time scales. My lab is
known for its biochemical reconstitution work in the budding yeast S. cerevisiae but more recently
we have done genomics, quantitative yeast cell microscopy with theoretical modeling, and
genome-wide screens in mammalian cells.
摘要
我的实验室主要有两个研究领域:1)尾锚定蛋白(TA)靶向和质量控制;
2)选择性自噬考虑到选择性自噬针对有毒蛋白质聚集体,
破坏细胞器,这两个研究领域有很强的概念联系。在
在许多情况下,我们都在回答关于底物选择性的类似问题:
新合成的TA蛋白作为TA的内质网蛋白靶向的底物
针对线粒体的蛋白质是什么区分TA蛋白,
线粒体作为天然线粒体TA蛋白质的质量控制底物?什么
区分明显受损的细胞器作为选择性自噬基板从健康
细胞器?为了回答这些问题,我们正在解剖不同的分子机制,
选择尺寸大不相同的衬底,并在非常不同的时间尺度上操作。我的实验室
以其在芽殖酵母S.但最近,
我们做了基因组学,定量酵母细胞显微镜与理论建模,
哺乳动物细胞的全基因组筛选。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Vladimir Denic其他文献
Vladimir Denic的其他文献
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{{ truncateString('Vladimir Denic', 18)}}的其他基金
Mechanistic dissection of eukaryotic protein biogenesis and degradation pathways
真核蛋白质生物发生和降解途径的机制剖析
- 批准号:
10623737 - 财政年份:2018
- 资助金额:
$ 56.99万 - 项目类别:
Uncovering kinase cascade mechanisms that target organelles for destruction by selective autophagy
揭示通过选择性自噬破坏靶向细胞器的激酶级联机制
- 批准号:
9215087 - 财政年份:2017
- 资助金额:
$ 56.99万 - 项目类别:
Defining the Essential Function of Heat Shock Factor and the Consequences of its Age-Associated Decline
定义热休克因子的基本功能及其与年龄相关的下降的后果
- 批准号:
8869633 - 财政年份:2015
- 资助金额:
$ 56.99万 - 项目类别:
Defining the Essential Function of Heat Shock Factor and the Consequences of its Age-Associated Decline
定义热休克因子的基本功能及其与年龄相关的下降的后果
- 批准号:
9050618 - 财政年份:2015
- 资助金额:
$ 56.99万 - 项目类别:
Mechanistic analysis of post-translation membrane protein insertion into the ER.
翻译后膜蛋白插入内质网的机制分析。
- 批准号:
8450736 - 财政年份:2012
- 资助金额:
$ 56.99万 - 项目类别:
Mechanistic analysis of post-translation membrane protein insertion into the ER.
翻译后膜蛋白插入内质网的机制分析。
- 批准号:
8219332 - 财政年份:2012
- 资助金额:
$ 56.99万 - 项目类别:
Mechanistic analysis of post-translation membrane protein insertion into the ER.
翻译后膜蛋白插入内质网的机制分析。
- 批准号:
8635374 - 财政年份:2012
- 资助金额:
$ 56.99万 - 项目类别: