Biomarkers of Early Experimental Glaucoma - Resubmission - 1

项目总结 青光眼是导致视力障碍和失明的主要原因，并对视网膜神经节造成不可逆转的损害 细胞。识别青光眼早期发现的生物标志物对于及时开始治疗至关重要 终生保存功能视力。该项目使用了慢性高眼压动物模型。 这为眼睛从健康到完全显露的转变提供了独特的洞察力 青光眼。利用先进的眼睛成像技术和最先进的图像处理方法，我们将 监测每种动物的纵向结构和功能变化，包括全局和局部变化 患上青光眼。我们将确定视神经对青光眼的两个主要原因的反应- 眼内压(IOP)和颅内压(ICP)-通过剧烈地调节这些压力和 量化随访过程中不同时间点视神经形变的变化。这 可以确定这些压力在青光眼发展和变化的不同阶段所起的作用 由于长期暴露于高眼压，导致视神经受损，也称为眼压重塑。最后，我们将 确定眼睛中与未来发展相关的结构和功能特征和位置 青光眼的损害。 该项目的结果将提供对敏感的眼部生物标志物及其时间表的洞察 青光眼的早期发现。我们的模型和工具可确保将结果转化为 人类对临床护理产生了实质性的影响。

PROJECT SUMMARY Glaucoma is a leading cause of vision morbidity and blindness with irreversible damage to retinal ganglion cells. Identifying biomarkers for early detection of glaucoma are paramount for timely initiation of treatment for preservation of functional vision throughout life. This project uses a chronic ocular hypertension animal model that provides unique insight into the changes the eye undergoes as it transitions from health to fully-manifest glaucoma. Using advanced ocular imaging technologies and state-of-the-art image processing methods we will monitor longitudinal structural and functional ocular changes, both global and local, of each animal as they develop glaucoma. We will determine the response of the optic nerve to two of the major causes of glaucoma – intraocular pressure (IOP) and intracranial pressure (ICP) – by acutely modulating these pressures and quantifying changes in optic nerve deformation at different time points along the course of the follow-up. This will allow determining the role of these pressures at the various stages of glaucoma development and changes in the optic nerve resulting from chronic exposure to elevated IOP, also known as remodeling. Lastly, we will identify structural and functional features and locations in the eye that are associated with future development of glaucomatous damage. The outcome of this project will provide insight into sensitive ocular biomarkers and their timeline of occurrence for early detection of glaucoma. Our model and tools ensure that the results can be translated to humans with substantial impact on clinical care.