Biomarkers of Early Experimental Glaucoma - Resubmission - 1

项目摘要 青光眼是导致视力下降和失明的主要原因之一，对视网膜神经节造成不可逆的损害 细胞鉴定用于早期检测青光眼的生物标志物对于及时开始治疗青光眼是至关重要的。 终身保持功能性视力。本课题采用慢性高眼压动物模型 提供了独特的洞察眼睛经历的变化，因为它从健康过渡到完全显现 青光眼利用先进的视觉成像技术和最先进的图像处理方法，我们将 监测每只动物的整体和局部的纵向结构和功能性眼部变化， 患上青光眼。我们将确定视神经对青光眼的两个主要原因的反应- 眼内压（IOP）和颅内压（ICP）-通过急性调节这些压力， 在随访过程中的沿着不同时间点量化视神经变形的变化。这 将允许确定这些压力在青光眼发展和变化的各个阶段的作用 在视神经中，由于长期暴露于升高的IOP，也称为重塑。最后，我们将 确定眼睛中与未来发育相关的结构和功能特征及位置 昏迷性损伤 该项目的结果将提供对敏感的眼部生物标志物及其时间轴的深入了解。 青光眼的早期诊断我们的模型和工具确保结果可以转化为 对临床护理有重大影响的人类。

PROJECT SUMMARY Glaucoma is a leading cause of vision morbidity and blindness with irreversible damage to retinal ganglion cells. Identifying biomarkers for early detection of glaucoma are paramount for timely initiation of treatment for preservation of functional vision throughout life. This project uses a chronic ocular hypertension animal model that provides unique insight into the changes the eye undergoes as it transitions from health to fully-manifest glaucoma. Using advanced ocular imaging technologies and state-of-the-art image processing methods we will monitor longitudinal structural and functional ocular changes, both global and local, of each animal as they develop glaucoma. We will determine the response of the optic nerve to two of the major causes of glaucoma – intraocular pressure (IOP) and intracranial pressure (ICP) – by acutely modulating these pressures and quantifying changes in optic nerve deformation at different time points along the course of the follow-up. This will allow determining the role of these pressures at the various stages of glaucoma development and changes in the optic nerve resulting from chronic exposure to elevated IOP, also known as remodeling. Lastly, we will identify structural and functional features and locations in the eye that are associated with future development of glaucomatous damage. The outcome of this project will provide insight into sensitive ocular biomarkers and their timeline of occurrence for early detection of glaucoma. Our model and tools ensure that the results can be translated to humans with substantial impact on clinical care.