Diversity Supplement to Elucidating the Orchestrated Bacterial Response to Copper Toxicity

阐明细菌对铜毒性反应的多样性补充

• 批准号: 10385571
• 负责人: Michael David Leslie Johnson
• 金额: $ 5.88万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10385571
• 关键词: Affect; Bacteria; Chemistry; Copper; Laboratories; Mediating; Metals; Molecular Chaperones; Nosocomial Infections; Nutritional Immunity; Research; Side; Streptococcus pneumoniae; Stress; Surface; System; Toxic effect; Work; pathogen; response; tool; toxic metal

Project Summary Copper is broadly toxic to bacteria despite being them being in contact since antiquity. Copper toxicity is seen in practice where copper surfaces and tools significantly reduce nosocomial infections and during host mediated nutritional immunity (sequestering essential metals while bombarding bacterial with toxic metals). While mechanisms of copper toxicity include Fenton chemistry and mismetallation, research is limited regarding these mechanisms are limited. Bacteria have a copper export system consisting of a repressor, a copper chaperone, and a copper exporter. The R35 this diversity supplement proposal is attached explores how bacteria, specifically Streptococcus pneumoniae evolved to interact with copper both from an effect and response point of view. Ms. Sanchez-Rosario has joined my laboratory and will work to elucidate these copper- specific mechanisms in S. pneumoniae on the side of bacterial responses.

项目摘要 铜对细菌有广泛的毒性，尽管自古以来就与细菌接触。可见铜毒性 在铜表面和工具显著减少医院感染的实践中， 介导的营养免疫（螯合必需金属，同时用有毒金属轰击细菌）。 虽然铜毒性的机制包括芬顿化学和错配，但研究有限 这些机制是有限的。细菌有一个铜输出系统，包括一个阻遏物， 一个铜的监护人，一个铜的出口商R35这一多样性补充建议是随附探讨 细菌，特别是肺炎链球菌是如何进化成与铜相互作用的， 回应的观点。桑切斯-罗萨里奥女士加入了我的实验室，将致力于阐明这些铜- S.肺炎对细菌的反应。