cryoEM Administrative Supplement for Equipment
cryoEM Administrative Supplement for Equipment
批准号:
10389992
负责人:
KENNETH ALLEN TAYLOR
金额:
$24.96万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-01-01 至 2025-12-31
关键词:
Administrative SupplementCollectionCryoelectron MicroscopyDataDefectElectronsEquipmentFilamentFundingGrantHeadImageInvertebratesLengthMechanicsMuscleMuscle functionMutationMyopathyMyosin ATPaseParatropomyosinPatternProteinsResolutionRoleSarcomeresStriated MusclesStructureTailThick FilamentThin FilamentThree-Dimensional ImageThree-Dimensional ImagingUrsidae FamilyVertebral columnVertebratesconnectindisease-causing mutationmoviemyosin-binding protein Cplasma protein Zreconstructionsensortool
中文摘要
摘要
英文摘要
ABSTRACT
Funds are requested for the purchase of a revolutionary new camera, the Apollo, from Direct
Electron Ltd. that would enable the collection of large amounts of image data, currently obtained
in the form of “movies”. The ability to collect large amounts of data would broaden the types of
questions addressable in the PI's R35 grant currently focused on obtaining atomic resolution
images of thick filaments from striated muscle. Generally, thick filaments from all striated
muscles have four structurally distinct regions: (1) a 160 nm long bare zone in the center where
myosin tails are packed antiparallel to each other, (2) an A-band follows where myosin
molecules are arranged in a helical or quasi helical structure with the myosin tails packed in a
parallel arrangement, (3) a tapered end where the connecting filament, e.g. titin, connects the
thick filament to the Z-disk, and (4) features along the thick filament A-band from invertebrates
in particular, but also from vertebrates, that fail to follow the myosin molecule arrangement. The
helical arrangement of myosin in the A-band of invertebrate thick filaments facilitates an atomic
structure of the myosin tail and head arrangement by using all of the myosin axial repeats from
the end of the bare zone to the beginning of the tapered ends. However, the bare zone, the
transition from bare zone to helical arrangement, the tapered ends as well as additional features
that do not follow the myosin helical symmetry perform important roles in the assembly of thick
filaments. Features, such as the paramyosin of invertebrate thick filaments fail to follow the
myosin helical symmetry in the A-band and are poorly represented in cryoEM reconstructions.
Paramyosin in concert with other non-myosin proteins may function to determine the thick
filament length in invertebrates. Titin, myosin binding protein C and other non-myosin proteins
of vertebrate thick filaments also do not follow a regular pattern along the entire A-band.
Acquisition of a much faster camera capable of recording a 60-frame movie in a single second,
will provide the tool needed to obtain high resolution 3-D images of these features that are not
well represented if at all in structures solved by enforcing helical symmetry.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
cryoEM Studies of Muscle
-
批准号:10551733
-
项目类别:
-
资助金额:$44.18万
-
财政年份:2021
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
cryoEM Studies of Muscle
-
批准号:10321535
-
项目类别:
-
资助金额:$44.18万
-
财政年份:2021
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
Purchase of a direct electron camera for the Titan-Krios at FSU
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批准号:8640487
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项目类别:
-
资助金额:$38.37万
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财政年份:2014
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负责人:KENNETH ALLEN TAYLOR
-
依托单位:
The CryoEM Motility Assay
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批准号:8464005
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项目类别:
-
资助金额:$15.71万
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财政年份:2012
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
The CryoEM Motility Assay
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批准号:8326253
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项目类别:
-
资助金额:$19.85万
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财政年份:2012
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负责人:KENNETH ALLEN TAYLOR
-
依托单位:
Purchase of a FEI Titan Krios for 3-D EM
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批准号:7498219
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项目类别:
-
资助金额:$200.0万
-
财政年份:2008
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
CryoEM Equipment Enhancements for Florida State University
-
批准号:7389329
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项目类别:
-
资助金额:$17.8万
-
财政年份:2008
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
STRUCTURE
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批准号:7313466
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项目类别:
-
资助金额:$14.81万
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财政年份:2006
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
PURCHASE OF A LARGE FORMAT CCD CAMERA FOR 3-D EM: INFECTIOUS DISEASE
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批准号:7166371
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项目类别:
-
资助金额:$6.49万
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财政年份:2005
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
PURCHASE OF A LARGE FORMAT CCD CAMERA FOR 3-D EM: AIDS
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批准号:7166372
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项目类别:
-
资助金额:$6.73万
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财政年份:2005
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
Purchase of a large format CCD camera for 3-D EM
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批准号:6877307
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项目类别:
-
资助金额:$24.03万
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财政年份:2005
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负责人:KENNETH ALLEN TAYLOR
-
依托单位:
PURCHASE OF A LARGE FORMAT CCD CAMERA FOR 3-D EM: CELL BIOLOGY
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批准号:7166373
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项目类别:
-
资助金额:$10.81万
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财政年份:2005
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
Gordon Reseach Conference on 3D EM of Macromolecules
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批准号:6601495
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项目类别:
-
资助金额:$0.67万
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财政年份:2003
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
Gordon Reseach Conference on 3D EM of Macromolecules
-
批准号:6710699
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项目类别:
-
资助金额:$0.0万
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财政年份:2003
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
3 D STRUCTURE OF THE HMM FRAGMENT OF MYOSIN II
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批准号:6699045
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项目类别:
-
资助金额:$20.81万
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财政年份:2001
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负责人:KENNETH ALLEN TAYLOR
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依托单位:
3 D STRUCTURE OF THE HMM FRAGMENT OF MYOSIN II
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批准号:6846012
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项目类别:
-
资助金额:$20.81万
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财政年份:2001
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负责人:KENNETH ALLEN TAYLOR
-
依托单位:
EM Studies of Dimeric Molecular Motors
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批准号:7211776
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项目类别:
-
资助金额:$31.61万
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财政年份:2001
-
负责人:KENNETH ALLEN TAYLOR
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依托单位:
EM Studies of Dimeric Molecular Motors
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批准号:7600476
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项目类别:
-
资助金额:$30.97万
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财政年份:2001
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
EM Studies of Dimeric Molecular Motors
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批准号:8064021
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项目类别:
-
资助金额:$29.44万
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财政年份:2001
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
EM Studies of Dimeric Molecular Motors
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批准号:7802198
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项目类别:
-
资助金额:$30.66万
-
财政年份:2001
-
负责人:KENNETH ALLEN TAYLOR
-
依托单位:
海外基金