Purchase of a large format CCD camera for 3-D EM

描述（由申请人提供）： 请求提供资金，用于购买一台大幅面CCD照相机，以加强佛罗里达州立大学的三维电子显微镜。该摄像机将安装在飞利浦/FEI CM 300-FEG上，取代现有的2k x 2k CCD摄像机，后者将转移到CM 120上。两个显微镜和两个CCD相机都被安置在一个共享的大学设施，称为生物科学成像资源（BSIR），其中包含许多研究人员在校园和北佛罗里达地区使用的光学和电子显微镜仪器。BSIR的专业人员由大学支付的员工组成，而不是用户费用，从而为那些只支付服务合同和消耗性用品费用的用户提供实质性支持。NIH资助的项目将通过该设备得到加强，包括以下项目：蛔虫精子运动复合体、SIV和HIV病毒、昆虫飞行肌、细胞粘附复合体和腺相关病毒的电子断层扫描。此外，该相机还将用于需要高通量数据收集的各种cryoEM 3-D成像项目。这些包括与各种受体复合的腺相关病毒，许多不同状态的重肌球蛋白，结核分枝杆菌的膜蛋白，以及在脂质单层上形成的各种2-D阵列。新的仪器将允许开发算法，以增加可以通过电子断层摄影术重建的区域，这可能需要对聚焦梯度和成像失真进行校正。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a large format CCD camera to enhance 3-D electron microscopy at Florida State University. The camera will be mounted on a Philips/FEI CM300-FEG and replace an existing 2k x 2k CCD camera which will be transferred to a CM120. Both microscopes and both CCD cameras are housed in a shared university facility, called the Biological Science Imaging Resource (BSIR), which contains instrumentation for both light and electron microscopy used by many researchers on campus and in the north Florida area. The BSIR is professionally staffed by employees that are paid by the university, not by user fees, thereby providing substantial support for users who only pay fees to cover service contracts and expendable supplies. NIH funded projects that will be enhanced by this equipment include the following: electron tomography of Ascaris sperm motility complexes, SIV and HIV viruses, insect flight muscle, cell adhesion complexes and adeno-associated virus. In addition the camera will find use in various cryoEM 3-D imaging projects that require high throughput data collection. These include adeno-associated virus in complex with various receptors, heavy meromyosin in many different states, membrane proteins from Mycobacterium tuberculosis, and 2-D arrays of various kinds formed on lipid monolayers. The new instrumentation will allow for development of algorithms for increasing the area that can be reconstructed by electron tomography which may need corrections for focus gradient and for imaging distortions.