Purchase of a large format CCD camera for 3-D EM

描述(由申请人提供)： 请拨资金在佛罗里达州立大学购买一台大幅面ccd相机，以增强3-D电子显微镜。该相机将安装在飞利浦/FEI CM300-FEG上，取代现有的2k x 2k CCD相机，后者将转移到CM120上。这两台显微镜和两台CCD摄像机都存放在一个名为生物科学成像资源(BSIR)的共享大学设施中，其中包含许多研究人员在校园和北佛罗里达地区使用的光学和电子显微镜仪器。BSIR的专业工作人员是由大学支付的员工，而不是用户费用，因此为只支付服务合同和消耗性用品费用的用户提供了大量支持。NIH资助的项目将通过这种设备得到加强，包括：蛔虫精子运动复合体的电子断层扫描，SIV和HIV病毒，昆虫飞行肌肉，细胞黏附复合体和腺相关病毒。此外，该相机还将用于各种需要高通量数据收集的CryoEM三维成像项目。这些包括与各种受体形成复合体的腺相关病毒、多种状态下的重肌球蛋白、结核分枝杆菌的膜蛋白，以及在脂单分子层上形成的各种二维阵列。新的仪器将允许开发算法，以增加可通过电子断层扫描重建的面积，这可能需要校正焦点梯度和成像失真。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a large format CCD camera to enhance 3-D electron microscopy at Florida State University. The camera will be mounted on a Philips/FEI CM300-FEG and replace an existing 2k x 2k CCD camera which will be transferred to a CM120. Both microscopes and both CCD cameras are housed in a shared university facility, called the Biological Science Imaging Resource (BSIR), which contains instrumentation for both light and electron microscopy used by many researchers on campus and in the north Florida area. The BSIR is professionally staffed by employees that are paid by the university, not by user fees, thereby providing substantial support for users who only pay fees to cover service contracts and expendable supplies. NIH funded projects that will be enhanced by this equipment include the following: electron tomography of Ascaris sperm motility complexes, SIV and HIV viruses, insect flight muscle, cell adhesion complexes and adeno-associated virus. In addition the camera will find use in various cryoEM 3-D imaging projects that require high throughput data collection. These include adeno-associated virus in complex with various receptors, heavy meromyosin in many different states, membrane proteins from Mycobacterium tuberculosis, and 2-D arrays of various kinds formed on lipid monolayers. The new instrumentation will allow for development of algorithms for increasing the area that can be reconstructed by electron tomography which may need corrections for focus gradient and for imaging distortions.