Functional causality in regulating cell morphogenesis

调节细胞形态发生的功能因果关系

• 批准号: 10387909
• 负责人: Gaudenz Danuser
• 金额: $ 20万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10387909
• 关键词: 3-Dimensional; Cell Shape; Cell physiology; Cells; Cost Sharing; Data; Data Analytics; Etiology; Funding; Image; Light; Microscope; Molecular; Morphogenesis; Process; Resolution; Signal Pathway; Three-Dimensional Imaging; experimental study; high resolution imaging; instrument; microscopic imaging; temporal measurement

ABSTRACT We request funds of $200K with an institutional cost sharing contribution of $150K to assemble a hyperspectral axially swept light sheet microscope for 3D imaging. The instrument will be tailored to the requirements of two new experiments that should generate high-resolution image data for analytical scrutiny of the causal relations between morphogenic signaling pathways and cell shape modulation. The microscope will support highly sensitive, quasi-simultaneous image acquisition of up to 7 spectrally distinct channels at very low light exposure and at a spatial resolution of 300 nm in XYZ and second scale temporal resolution.

摘要 我们要求20万美元的资金，其中15万美元的机构成本分摊捐款， 用于3D成像的高光谱轴向扫描光片显微镜。该工具将根据 两个新实验的要求，应产生高分辨率的图像数据，分析审查 形态发生信号通路与细胞形状调节之间的因果关系。显微镜将 支持高灵敏度、准同时图像采集，最多可采集7个光谱清晰通道， 曝光，XYZ中的空间分辨率为300 nm，第二尺度时间分辨率。