Deciphering Wnt-Ror signaling in cytoskeletal regulation and tissue shape control

补充摘要 我正在申请购买BD Accuri C6 Plus流式细胞仪的管理补充。这 流式细胞仪配备有两个激光器和一个自动取样器，能够一次处理24个样品。这 设备将是完成我的NIGMS R35赠款（R35 GM 119574）的目标，通过提高 我们实验室在R35资助的支持下开发了一种新型报告基因检测方法， Wnt 5a-Ror形态发生途径的生化机制。通过将该报告基因测定与 新优化的基因置换范式，我们已经开始分析大量的突变蛋白质 与通路功能障碍和Robinow综合征发病机制有关。我们的工作流程现在 超过了目前可用的流式细胞仪的容量，流式细胞仪不再能处理大量的 分析效率，因此需要一个自动化的系统。细胞生物学与人类学系 加州大学戴维斯分校医学院的解剖学致力于提供空间来容纳细胞仪和资金， 仪器服务。总之，所要求的流式细胞仪将大大加快NIGMS资助的项目 在我的实验室，以及其他国家卫生研究院的研究人员在该部门和更大的加州大学戴维斯分校社区。

Supplement Summary I am requesting an administrative supplement for the purchase of a BD Accuri C6 Plus Flow Cytometer. This cytometer is equipped with two lasers and an automatic sampler capable of handling 24 samples at once. This equipment will be crucial for completing the goals of my NIGMS R35 grant (R35GM119574) by enhancing the throughput of a novel reporter assay that our lab developed, under the auspices of this R35 grant, to investigate the biochemical mechanisms of the Wnt5a-Ror morphogenetic pathway. By coupling this reporter assays with a newly optimized gene-replacement paradigm, we have begun to analyze a large number of mutant proteins associated with dysfunction of the pathway and Robinow syndrome pathogenesis. Our workflow has now exceeded the capacities of the currently available flow cytometers which can no longer handle the volume of analyses efficiently, hence the new need for an automated system. The Department of Cell Biology and Human Anatomy at UC Davis School of Medicine is committed to provide space to house the cytometer and funds for instrument service. In summary, the requested flow cytometer will greatly accelerate the NIGMS-funded projects in my lab, as well as those of other NIH investigators in the department and the greater UC Davis community.