Deciphering Wnt-Ror signaling in cytoskeletal regulation and tissue shape control

补充摘要 我要求购买BD Accuri C6 Plus流式细胞仪的行政补充。这 细胞仪配备了两个激光器和一个能够同时处理24个样本的自动取样器。这 设备对于完成我的NIGMS R35奖助金(R35GM119574)的目标至关重要，因为它增强了 我们实验室在这笔R35拨款的支持下开发的一种新的报告分析的吞吐量 Wnt5a-RoR形态发生途径的生化机制。通过将记者的分析与一个 最新优化的基因替换范式，我们已经开始分析大量的突变蛋白质 与该通路功能障碍和Robinow综合征的发病机制有关。我们的工作流程现在已经 超过了当前可用的流量细胞仪的容量，该流量计不再能够处理 高效的分析，因此对自动化系统的新需求。细胞生物学与人类系 加州大学戴维斯分校医学院的解剖学致力于为细胞仪提供空间和资金 仪器服务。总之，所要求的流式细胞仪将极大地加速NIGMS资助的项目 在我的实验室，以及NIH部门和加州大学戴维斯分校社区的其他调查人员。

Supplement Summary I am requesting an administrative supplement for the purchase of a BD Accuri C6 Plus Flow Cytometer. This cytometer is equipped with two lasers and an automatic sampler capable of handling 24 samples at once. This equipment will be crucial for completing the goals of my NIGMS R35 grant (R35GM119574) by enhancing the throughput of a novel reporter assay that our lab developed, under the auspices of this R35 grant, to investigate the biochemical mechanisms of the Wnt5a-Ror morphogenetic pathway. By coupling this reporter assays with a newly optimized gene-replacement paradigm, we have begun to analyze a large number of mutant proteins associated with dysfunction of the pathway and Robinow syndrome pathogenesis. Our workflow has now exceeded the capacities of the currently available flow cytometers which can no longer handle the volume of analyses efficiently, hence the new need for an automated system. The Department of Cell Biology and Human Anatomy at UC Davis School of Medicine is committed to provide space to house the cytometer and funds for instrument service. In summary, the requested flow cytometer will greatly accelerate the NIGMS-funded projects in my lab, as well as those of other NIH investigators in the department and the greater UC Davis community.