Image Acquisition and Analysis Core

图像采集和分析核心

• 批准号: 10394406
• 负责人: Ian A Sigal
• 金额: $ 24.7万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10394406
• 关键词: Biological Assay; Caring; Core Grant; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Development; Equipment; Ethical Analysis; Ethics; Experimental Designs; Faculty; Fluorescence; Funding; Goals; Image; Image Analysis; Knowledge; Laboratories; Methodology; Methods; Microscopy; Optical Coherence Tomography; Phase; Protocols documentation; Research Personnel; Research Project Grants; Technical Expertise; Training; Vision; Vision research; base; data acquisition; data integrity; experience; imaging modality; success; theories

SUMMARY - IMAGE ACQUISITION AND ANALYSIS MODULE The Image Acquisition and Analysis Module provides our Participating Faculty and their laboratory staff access to state-of-the-art equipment and technical support for highly sophisticated imaging and analyses. This equipment and support is essential for the success of numerous NEI-funded projects, and for new faculty to acquire critical preliminary data for R01 applications. Vision research projects involving imaging require expert knowledge of different acquisition methods; brightfield, darkfield, fluorescence, phase contrast and DIC microscopy as well as optical coherence tomography. Also required is understanding of the theory underlying the acquisition of the data, storage, transfer and interpretation of the data in order to to maintain the integrity of the data for ethical analysis and quantification. These latter aspects create a significant barrier to those with little experience in imaging but who wish to employ imaging-based methodologies in their vision research, and they can also hinder the progress of even the most experienced operator if the equipment is not well maintained. The Image Acquisition and Analysis Module provides the theoretical and practical training necessary to overcome the former of these barriers, and the technical expertise and care required to manage the complexities of equipment utilized for a variety of imaging modalities.

摘要-图像采集和分析模块 图像采集和分析模块为我们的参与教师及其实验室工作人员提供访问 到最先进的设备和技术支持，以实现高度复杂的成像和分析。这 设备和支持对于NEI资助的众多项目的成功以及对新教师来说是必不可少的 获取R01应用程序的关键初步数据。涉及成像的视觉研究项目需要专家 了解不同的采集方法；明场、暗场、荧光、相位对比度和DIC 显微镜以及光学相干层析成像。还需要对其背后的理论有所了解 数据的采集、存储、传输和解释，以维护数据的完整性 用于伦理分析和量化的数据。这些后几个方面对那些几乎没有 在成像方面有经验，但希望在视觉研究中使用基于成像的方法，并且他们 如果设备没有得到很好的维护，即使是最有经验的操作员也会阻碍进度。这个 图像采集和分析模块提供必要的理论和实践培训，以克服 这些障碍中的前者，以及管理复杂性所需的技术专长和关注 用于各种成像模式的设备。