Genomics

项目摘要/摘要：基因组学共享资源(GSR) 基因组共享资源(GSR)的目标是提供先进的基因组学技术和服务 需要支持在亚利桑那大学癌症中心进行的全面癌症研究 (UACC)。单基因到全基因组分析是癌症生物学研究和发现的基石之一。 GSR为UACC成员提供了指导、高质量、经济高效的访问全方位的 基因组基石技术。GSR提供以下服务：1)下一代测序；2) 表观遗传分析；3)基因表达谱；4)全基因组DNA分析；以及5)基因组DNA 差异。在这些服务类别中，技术包括全基因组表达图谱， 比较基因组杂交，基于序列的拷贝数变异，整个外显子组测序， 鸟枪测序、靶向测序面板、RNA-SEQ、染色质免疫沉淀(CHIP)-SEQ， 基因分型、表观基因组芯片分析、实时定量聚合酶链式反应(QPCR)、样品质量 保证/质量控制(QA/QC)、样品隔离、实验咨询、数据分析和数据 存档。具有分析单基因/小基因集以及全基因组分析的能力，GSR 在分析方法上提供了极大的灵活性。此外，GSR还提供了开发 定制应用程序并提供新技术，以满足UAcc的特定需求 调查人员。GSR设备包括：两台Ion Torrent个人基因组机器，一台Ion Torrent质子 (用于小型到大型下一代测序需求)、Affymetrix和安捷伦微阵列平台(用于 以杂交为基础的服务)和三台应用生物系统qPCR机(提供全面服务和自助服务 聚合酶链式反应应用)。GSR提供实验设计和样品制备方面的咨询，其次是 为分析做准备的QA/QC样本。数据分析和存储对调查人员的能力至关重要 获取他们的数据以供发布和传播。因此，GSR提供微阵列、序列和 进行定量聚合酶链式反应分析，以支持出版物和赠款的准备工作。此外，该服务还提供访问 通过受密码保护的网站获取原始数据，并对调查员的数据进行三倍冗余备份 五年了。

Project Summary / Abstract: Genomics Shared Resource (GSR) The goal of the Genomics Shared Resource (GSR) is to provide advanced genomics technology and services required to support the full range of cancer research conducted at the University of Arizona Cancer Center (UACC). Single gene to full genome analyses is one cornerstone of cancer biology research and discovery. The GSR provides UACC Members with guided, high-quality, cost-efficient access to a full spectrum of genomic cornerstone technologies. The GSR delivers the following services: 1) next-generation sequencing; 2) epigenetic analysis; 3) gene expression profiling; 4) genome-wide DNA analysis; and 5) genomic DNA variance. Within these categories of service, techniques include whole genome expression profiling, comparative genomic hybridization, sequence-based copy number variance, whole exome sequencing, shotgun sequencing, targeted sequencing panels, RNA-seq, chromatin immunoprecipitation (ChIP)-seq, genotyping, epigenomic ChIP-chip assays, real-time quantitative PCR (qPCR), sample quality assurance/quality control (QA / QC), sample isolation, experimental consultation, data analysis, and data archiving. With the ability to analyze single gene/small gene sets as well as genome wide analyses, the GSR offers great flexibility in analytical approaches. In addition, the GSR provides the capability for developing custom applications and makes new technologies available to address the specific needs of UACC investigators. GSR equipment includes: two Ion Torrent Personal Genome Machines, an Ion Torrent Proton (for small to large next-generation sequencing needs), Affymetrix and Agilent microarray platforms (for hybridization-based services), and three Applied Biosystems qPCR machines (for full service and self-service PCR applications). The GSR provides consultation on experiment design, and sample preparation, followed by sample QA / QC in preparation for the analysis. Data analysis and storage are critical to investigator's ability to access their data for publication and dissemination. Accordingly, the GSR provides microarray, sequence, and qPCR analysis to support the preparation of publications and grants. In addition, the Service provides access to raw data through a password protected website, along with triply redundant backup of investigator's data for five years.