Animal, Biochemical/Molecular and Morphology Core

项目摘要-核心B 这个项目的总体主题是确定交感神经激活的潜在机制。 由慢性间歇性缺氧(CIH)引起，这是睡眠呼吸暂停的主要表现。所有项目都需要 将未服用镇静剂的完整啮齿动物或细胞培养物暴露在模拟血氧饱和度的IH范例中 在睡眠呼吸暂停期间遇到的档案。在所有项目中使用相同的IH范例是至关重要的，因为它 允许纵向整合结果。这一目标最好是由一个集中的科学设施来实现。 所有的项目都使用了转基因小鼠。Science Core为集中式设施提供专用的 监督基因分型和维持繁育群体的科学人员，否则这是成本 对个别项目来说令人望而却步。这个项目中的所有项目都提出了各种生化和分子 生物学分析以及在项目中常见的形态研究。进行这些化验 根据科学核心是经济的，并允许使用常见的试剂；抗体等 重要的是提供质量控制。因此，科学核心的总体目标是支持集中化 为所有四个拟议项目提供科学服务。以下是科学核心的具体目标。 目的1：为动物和细胞培养提供间歇性低氧暴露的集中化设施。目标2： 提供生化和分子生物学检测。目的3：提供形态分析方法。目标4： 提供数据统计分析方面的帮助。科学核心将由彭永杰博士执导，他是 经验丰富的心肺生理学家。主要人员包括：J.Nanduri博士(专业知识--生化 和分子生物学方法，A.J.加西亚(通过靶向基因破坏产生专门知识的小鼠) 和D.Nicolae(专门知识--统计和计算建模和分析)。主要人员将是 由S.A.Khan博士、N.Wang女士和B.Wang先生等工作人员协助。科学的核心是 位于芝加哥大学综合生理学研究所(IIP)，那里的大多数 将进行拟议的研究。

Project Summary- Core B The overall theme of this program is to determine the mechanisms underlying sympathetic nerve activation caused by chronic intermittent hypoxia (CIH), a major manifestation of sleep apnea. All projects require exposing either intact, un-sedated rodents or cell cultures to an IH paradigm mimicking the blood O2 saturation profiles encountered during sleep apnea. Use of the same IH paradigm across all projects is essential as it allows longitudinal integration of results. This goal will be best accomplished by a centralized scientific facility. All projects utilize genetically engineered mice. Scientific Core provides centralized facilities with dedicated scientific personnel overseeing the genotyping and maintaining breeding colonies, which otherwise is cost prohibitive for individual projects. All projects in this program propose a variety of biochemical and molecular biology assays as well as morphology studies that are common amongst projects. Performing these assays under the Scientific Core is economical and allows use of the common reagents; antibodies etc. and more importantly provide quality control. Thus, the overall goal of the Scientific Core is to support centralized scientific services to all four projects proposed. The following are the SPECIFIC AIMS of the Scientific Core. AIM 1: Provide centralized facility for exposure of animals and cell cultures to intermittent hypoxia. AIM 2: Provides biochemical and molecular biological assays. AIM 3: Provide morphology assays. AIM 4: Provide help with statistical analysis of the data. The Scientific Core will be directed by Dr. Y-J- Peng, an experienced cardio-respiratory physiologist. Key Personnel include: Drs. J. Nanduri (expertise- biochemical and molecular biology approaches, A.J. Garcia (expertise- generating mice with targeted disruption of genes) and D. Nicolae (expertise- statistical and computational modeling and analyses). Key personnel will be assisted by Staff Members including Dr. S.A. Khan, Ms. N. Wang and Mr. B. Wang. The Scientific Core is situated at the Institute for Integrative Physiology (IIP) of the University of Chicago, where most of the proposed research will be conducted.