Animal, Biochemical/Molecular and Morphology Core

项目摘要-核心B 这个项目的总体主题是确定交感神经激活的潜在机制 由慢性间歇性缺氧（CIH）引起，这是睡眠呼吸暂停的主要表现。所有项目都需要 将完整的、未镇静的啮齿动物或细胞培养物暴露于模拟血氧饱和度的IH范例 睡眠呼吸暂停综合征的症状在所有项目中使用相同的IH范式是必不可少的，因为它 允许纵向整合结果。这一目标最好由一个集中的科学设施来实现。 所有项目都使用基因工程小鼠。Scientific Core提供集中式设施， 科学人员监督基因分型和维持繁殖群体，否则是成本 对于个别项目来说，该计划中的所有项目都提出了各种生物化学和分子生物学 生物分析以及项目中常见的形态学研究。执行这些测定 在科学核心下的是经济的，并允许使用常见的试剂;抗体等。 重要是提供质量控制。因此，科学核心的总体目标是支持集中的 为所有四个项目提供科学服务。以下是科学核心的具体目标。 目的1：提供集中的设施，用于动物和细胞培养物暴露于间歇性缺氧。目标2： 提供生物化学和分子生物学分析。目的3：提供形态学分析。目标4： 帮助进行数据的统计分析。科学核心将由Y-J-Peng博士指导， 经验丰富的心肺生理学家。主要人员包括：Drs. J. Nanduri（专业知识-生物化学 和分子生物学方法，A.J. Garcia（专门知识-产生基因靶向破坏的小鼠） 和d. Nicolae（专业知识-统计和计算建模和分析）。关键人员将 由包括S.A.博士在内的工作人员协助。Khan，N女士王先生和B先生。王.科学核心是 位于芝加哥大学综合生理学研究所（IIP）， 将进行拟议的研究。

Project Summary- Core B The overall theme of this program is to determine the mechanisms underlying sympathetic nerve activation caused by chronic intermittent hypoxia (CIH), a major manifestation of sleep apnea. All projects require exposing either intact, un-sedated rodents or cell cultures to an IH paradigm mimicking the blood O2 saturation profiles encountered during sleep apnea. Use of the same IH paradigm across all projects is essential as it allows longitudinal integration of results. This goal will be best accomplished by a centralized scientific facility. All projects utilize genetically engineered mice. Scientific Core provides centralized facilities with dedicated scientific personnel overseeing the genotyping and maintaining breeding colonies, which otherwise is cost prohibitive for individual projects. All projects in this program propose a variety of biochemical and molecular biology assays as well as morphology studies that are common amongst projects. Performing these assays under the Scientific Core is economical and allows use of the common reagents; antibodies etc. and more importantly provide quality control. Thus, the overall goal of the Scientific Core is to support centralized scientific services to all four projects proposed. The following are the SPECIFIC AIMS of the Scientific Core. AIM 1: Provide centralized facility for exposure of animals and cell cultures to intermittent hypoxia. AIM 2: Provides biochemical and molecular biological assays. AIM 3: Provide morphology assays. AIM 4: Provide help with statistical analysis of the data. The Scientific Core will be directed by Dr. Y-J- Peng, an experienced cardio-respiratory physiologist. Key Personnel include: Drs. J. Nanduri (expertise- biochemical and molecular biology approaches, A.J. Garcia (expertise- generating mice with targeted disruption of genes) and D. Nicolae (expertise- statistical and computational modeling and analyses). Key personnel will be assisted by Staff Members including Dr. S.A. Khan, Ms. N. Wang and Mr. B. Wang. The Scientific Core is situated at the Institute for Integrative Physiology (IIP) of the University of Chicago, where most of the proposed research will be conducted.