Andor Dragonfly 201 Spinning Disk Confocal Microscope System

项目摘要/摘要： 这项基本仪器补助计划的目的是申请购买Andor蜻蜓的资金 201旋转圆盘共聚焦显微镜系统将安装在罗文大学科学礼堂大楼， 格拉斯伯勒校区。收购这种最先进的仪器将满足对高分辨率的迫切需求 由美国国立卫生研究院资助的越来越多的罗文研究人员的共聚焦显微镜。所有参赛者 调查人员表示，该工具不仅需要支持他们各自的NIH- 资助的项目只是为了扩大和加强他们的科学目标和对罗文学生的培训。在 在科学与数学学院(CSM)没有共焦显微镜，并且可以访问 这样的仪器仅限于我们罗文的理科教职员工和学生。因此，随着收购 Andor蜻蜓201，我们计划在我们的科学大楼中创建一个带有共焦的成像核心设施 以显微镜为中心，罗文、美国国立卫生研究院资助的其他研究人员可以广泛接触到这一点 仪器仪表。Andor蜻蜓201是一款高速旋转的多点共聚焦扫描仪，将 配备四个多激光器(405 nm/521 nm/594 nm/698 nm)，BorealisTM照明系统，以及 强大的Zyla 4.2 plus探测器，将导致高灵敏度和低光漂白的3D成像。这个 该系统还将包括配备全电动XY扫描工作台的徕卡DMi8倒置显微镜 以及4个发射滤光片，允许研究人员一次进行3到4个颜色标记实验。《蜻蜓》 201系统还包括一个配备Fusion和综管信息系统软件的工作站，不仅用于 获取和可视化3D图像，但要允许实时查看光学部分，请添加 在捕获过程中使用ClearView-GPUTM去卷积算法增强分辨率，并缝合图像 在捕获相邻视场后立即平铺。融合缝纫机导致完美的瓷砖 3D图像的蒙太奇，具有出色的一致性、分辨率，并且没有显示帧边界。 美国国立卫生研究院资助的研究项目包括：(1)分子基础和贡献 染色质结构对精子发生的影响；(2)与浦肯野相关的细胞和分子机制 细胞发育和神经变性；(3)实验性干预对大鼠神经炎症的影响 物质滥用模型；(4)与行为灵活性缺陷相关的神经回路发病机制 物质滥用和阿尔茨海默病的模型；(5)识别和定位参与 细菌生物膜的形成。其他项目将使用这种共聚焦技术来确定细胞的贡献 黏附分子进入病毒和乳腺癌，并监测硅量子的命运和疗效 DOTS作为细胞和组织中的双模式靶向造影剂。总体而言，收购Andor蜻蜓 201将有力地推动罗文CSM研究人员和学生的研究目标，并做出贡献 罗文大学正在经历的生物医学研究的持续增长。 1

Project Summary/Abstract: The purpose of this Basic Instrumentation Grant proposal is to request funds to purchase the Andor Dragonfly 201 Spinning Disk Confocal Microscope System to be housed in the Science Hall building at Rowan University, Glassboro campus. The acquisition of this state-of-the-art instrument will fill a critical need for high-resolution confocal microscopy for a growing group of Rowan investigators funded by the NIH. All the participating investigators have indicated the significant need for the instrument not only to support their respective NIH- funded projects but to expand and strengthen their scientific goals and training of Rowan students. At the moment there is no confocal microscope at the College of Science & Mathematics (CSM), and accessibility to such an instrument is limited to our science faculty and students at Rowan. Therefore, with the acquisition of the Andor Dragonfly 201, we plan to create an imaging core facility in our science building with the confocal microscope as the centerpiece where Rowan NIH-funded and other investigators can have wide access to this instrumentation. The Andor Dragonfly 201 is a high-speed spinning multipoint confocal scanner that will be equipped with four multi-lasers (405nm/521nm/594nm/698nm), the BorealisTM illumination system, and the potent Zyla 4.2 PLUS detector that will result in high sensitivity and low photobleaching 3D imaging. The system will also include a Leica DMi8 inverted microscope equipped with a fully motorized XY scanning stage and 4 emission filters to allow investigators to perform 3 to 4 color labeling experiments at once. The Dragonfly 201 system also includes a workstation equipped with the Fusion and IMARIS software not only for the acquisition and visualization of the 3D images, but to allow real-time viewing of the optical sections, add resolution enhancement during capture using the ClearView-GPUTM Deconvolution algorithm, and stitch image tiles immediately after adjacent fields of view are captured. The Fusion stitcher leads to perfectly tiled montages of 3D images with excellent uniformity, resolution, and without the showing of frame boundaries. NIH-funded research projects to be supported by this instrument include: (1) Molecular basis and contribution of chromatin architecture to spermatogenesis; (2) Cellular & molecular mechanisms associated with Purkinje cell development and neurodegeneration; (3) Effects of experimental interventions on neuroinflammation in models of substance abuse; (4) Neural circuitry pathogenesis associated with deficits in behavioral flexibility in models of substance abuse and Alzheimer’s disease; (5) Identifying and targeting chemical signals involved in bacterial biofilm formation. Other projects will use this confocal technology to determine the contribution of cell adhesion molecules to viral entry and breast cancer, and to monitor the fate and efficacy of Silicon quantum dots as dual-mode targeted contrast agents in cells and tissues. Overall, the acquisition of the Andor Dragonfly 201 will strongly advance the research goals of investigators and students in the CSM at Rowan and contribute to the ongoing growth in biomedical research that Rowan University is experiencing. 1