Precision Histology

精密组织学

• 批准号: 10413912
• 负责人: PARTHA Pratim MITRA
• 金额: $ 45.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413912
• 关键词: 3-Dimensional; Anatomy; Atlases; Brain; Brain Stem; Data; Data Set; Exploratory Behavior; Histologic; Histology; Image; Individual; Label; Laboratories; Lead; Methods; Motor; Mus; Neurons; Population; Research Project Grants; Reverse engineering; Slice; Spinal; Stains; Standardization; System; Texture; Tissues; Training; Transgenic Mice; base; brain tissue; cell type; digital; experimental study; histological stains; in vivo; motor control; novel marker; olfactory bulb; operation; orofacial; reconstruction

PIs: Martin Deschênes, Yoav Freund, David Golomb, David Kleinfeld (lead), Fan Wang ! Core 2. Abstract Precision Histology This Core provides high-throughput tape transfer sectioning to cut near perfect stacks of slices of the whole brain, from olfactory bulb to spinal level C2. The tape transfer histology system has been in continuous operation in the Mitra laboratory for several years and will enable the registration of anatomical data from Research Projects 1, 2, and 3, into a common framework established by the Trainable Texture-based Digital Atlas. Control of orofacial actions is coordinated by distinct populations of brain stem premotor neurons that are arranged into relatively small clusters and limited to domains as small as 200 to 300 µm in extent. For many orofacial motor actions, premotor neuronal clusters are present at multiple levels of the brainstem and do not conform to the boundaries previously defined by available atlases, including the Paxinos atlases and the Allen Brain Common Coordinate Framework atlas. As part of our concerted effort to identify the functional organization of brainstem motor control, we require standardized processing of brain tissue. The established histology pipeline in the Mitra laboratory offers key advantages over alternative methods, in that tissue is retained in near perfect alignment during sectioning and is amenable to standard histological stains that demarcate brainstem landmarks used to align and annotate brains. Crucially, this approach precludes distortions from classical sectioning methods and enables efficient 3D reconstructions, cross-brain registration, and efficient incorporation of data from new brains into our recently developed Trainable Texture-based Digital Atlas. By efficiently multiplexing labeled neurons from transsynaptic tracing, in vivo recordings, and transgenic mice/cell type marker stains, the Precision Histology Core serves to support data compilation into a common anatomical framework.

PIS：马丁·德谢恩斯，约夫·弗洛因德，大卫·戈罗姆，大卫·克莱因菲尔德(领衔)，王帆 好了！ 核心2.摘要 精密组织学 该机芯提供高吞吐量的磁带传输分段，以切割出近乎完美的整体切片堆叠 大脑，从嗅球到脊柱水平C2。胶带转移组织学系统一直在连续进行 在Mitra实验室运行数年，并将能够注册来自 研究项目1、2和3，纳入可培训的基于纹理的数字技术建立的公共框架 阿特拉斯。 口面部动作的控制由不同的脑干前运动神经元群体协调，这些神经元是 排列成相对较小的簇，并限于范围小至200至300微米的域。对许多人来说 口面部运动，运动前神经元团存在于脑干的多个水平上，而不是 遵守先前由现有地图集定义的边界，包括帕西诺斯地图集和艾伦地图集 大脑共同坐标框架图集。 作为我们共同努力确定脑干运动控制的功能组织的一部分，我们需要 脑组织的标准化处理。Mitra实验室建立的组织学管道提供了关键 与其他方法相比的优点在于，组织在切片过程中保持近乎完美的对准，并 符合标准组织学染色，用于划分用于对齐和注释的脑干标志物 大脑。最重要的是，这种方法避免了经典分割方法的扭曲，并使 3D重建、跨脑注册，以及将来自新大脑的数据高效地整合到我们的 最近开发了可训练的基于纹理的数字地图集。通过高效地将标记神经元从 跨突触追踪，活体记录，转基因小鼠/细胞类型标记染色，精密组织学 CORE用于支持将数据汇编到通用解剖框架中。