Spectral Cell Sorter

光谱细胞分选仪

• 批准号: 10415701
• 负责人: Albert K Tai
• 金额: $ 67.24万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2023-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415701
• 关键词: Air; Biological Sciences; Biology of Aging; Cancer Biology; Cell Separation; Cells; Cellular biology; Communities; Containment; Core Facility; Custom; Cytometry; Decision Making; Detection; Developmental Biology; Discipline; Disease; Equipment; Financial Support; Flow Cytometry; Funding; Genetic; Health Sciences; Immunology; Interruption; Laboratories; Lasers; Microbiology; Mission; Neurobiology; Process; Research; Research Personnel; Science; Services; Sorting - Cell Movement; Technology; United States National Institutes of Health; Universities; Vendor; Work; experimental study; instrument; instrumentation; member; nervous system disorder; pathogen; prevent; service providers; success

The Tufts Laser Cytometry (TLC) Core Facility is applying for funds to purchase a Bigfoot Spectral Cell Sorter (ThermoFisher Scientific). This instrument will be a critical acquisition, allowing TLC to maintain its mission of providing high-quality and up-to-date flow cytometry services to the research community within the extended Tufts University campus network. TLC was established in 1998 and has continued its status as the only Flow Cytometry Core facility at Tufts University ever since. TLC is an indispensable service provider to the science community. Its success in supporting high-impact NIH-funded research can be largely attributed to its outstanding and dedicated staff members, Mr. Allen Parmelee and Mr. Stephen Kwok, as well as the continuous institutional (financial) support. In the past two years, 53 laboratories used the Core's sorting service. The majority of them are NIH- funded, with several others actively seeking initial or renewed NIH funding. Researchers that rely on the TLC work in health- and life-sciences disciplines including immunology, microbiology & host-pathogen interaction, cellular & cancer biology, genetic & associated diseases, neurobiology & neurologic diseases, developmental biology, and aging. TLC currently offers its services via two suites of instrumentation. The Analytic instrument suite includes a FACSCalibur and an LSRII (both BD), along with a new Aurora Spectral Flow Cytometer (Cytek). The Sorter instrument suite includes a MoFlo, a legacy Influx with biosafety containment (both 15+ yrs old), and a Cytek customized BD FACSAria II (Cytek-Aria, 10+ yrs). The suite of Sorter instruments is outdated and increasingly unreliable. By the end of 2021, none of the current sorters in TLC will be serviceable by their vendors. To prevent interrupting the critical sorting service to NIH-funded users, a new cell sorter will be essential. The TLC team considered the current and anticipated needs of its users and both of our Suite' capabilities and determined that the Bigfoot Sorter is the only logical choice. Part of the decision-making process pivots on harmonizing the capabilities of Analytic and Sorter equipment already in the facility. For instance, the current Cytek-Aria compliments the specifications of the LSRII. This approach allows researchers to migrate experiments optimized on the analytic LSRII to the Cytek-Aria for cell sorting. Acquiring the Aurora analytic cytometer allowed users to capitalize on the cutting-edge technology of analytic cytometry with complexity comparable to the CyTOF (Mass Spectometry, but in a far more economical platform). The key features of the Bigfoot include: jet-in-air sorting; built-in, certified bio-containment; and is the only instrument with both “conventional” and “spectral” detection modes. This machine would allow the dovetailing of the analytic and sorting functionalities and reproduce the specifications of the facility's current sorters. It will advance state-of- the-art cytometry to users and provide much-needed support in maintaining the current service levels.

塔夫茨激光细胞仪(TLC)核心设备正在申请资金购买一台大脚光谱细胞分选器 (ThermoFisher Science)。这一仪器将是一次关键的收购，使TLC能够保持其 为研究社区提供高质量和最新的流式细胞仪服务 塔夫茨大学校园网。 TLC成立于1998年，一直是塔夫茨唯一的流式细胞仪核心设施 从那以后就上了大学。TLC是科学界不可或缺的服务提供者。它在中国取得的成功 支持NIH资助的高影响力研究在很大程度上要归功于其杰出而敬业的员工 成员、郭炳江先生和郭炳江先生，以及连续机构(金融) 支持。在过去的两年里，有53个实验室使用了Core的分类服务。他们中的大多数是NIH- 资金，还有其他几个人积极寻求NIH的初始或续签资金。依赖TLC的研究人员 从事健康和生命科学学科的工作，包括免疫学、微生物学和宿主-病原体相互作用， 细胞与癌症生物学、遗传与相关疾病、神经生物学与神经疾病、发育 生物学和衰老。 TLC目前通过两套仪器提供服务。分析仪器套件包括 FACSCalibur和LSRII(均为BD)，以及新的极光光谱流式细胞仪(Cytek)。《分拣者》 仪器套件包括MoFlo和Cytek，这是一种带有生物安全封堵装置(都有15年以上的历史)的传统流入设备 定制BD FACSAria II(Cytek-Aria，10年以上)。Sorter仪器套装已经过时，而且越来越多 不可靠。到2021年底，TLC中目前的分拣机都不能由其供应商提供服务。至 为了防止中断对NIH资助用户的关键分选服务，一个新的细胞分选机将是必不可少的。薄层色谱 团队考虑了其用户的当前和预期需求，以及我们套件的功能和 确定大脚分类程序是唯一合乎逻辑的选择。决策过程的一部分取决于 协调设施中已有的分析和分选设备的能力。例如，当前的 Cytek-Aria对LSRII的规格表示赞赏。这种方法允许研究人员迁移 实验优化了对Cytek-Aria进行细胞分选的LSRII解析方法。获得Aurora分析 细胞仪使用户能够充分利用分析细胞仪的尖端技术 可与CyTOF(质谱仪，但在更经济的平台)相媲美。的主要功能 BigFoot包括：空中喷射分拣；内置、认证的生物容器；是唯一同时具备这两种功能的仪器 “常规”和“光谱”检测模式。这台机器将允许分析和分析 分拣功能，并复制该设施当前分拣机的规格。它将推动国家- 向用户提供最先进的细胞仪，并在维持当前服务水平方面提供亟需的支持。