High Speed, High Resolution Slide Scanner for Research in Translational Medicine

用于转化医学研究的高速、高分辨率幻灯片扫描仪

• 批准号: 10415257
• 负责人: JOHN S CONDEELIS
• 金额: $ 47.19万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415257
• 关键词: Biomedical Research; Carcinoma; Communities; Disease; Disseminated Malignant Neoplasm; Enhancement Technology; Environment; Funding; Gene Expression Profile; Genetic Transcription; Grant; Hepatocyte; Heterogeneity; Image; Immunocompromised Host; Interdisciplinary Study; Liver; Medicine; Microscope; Microscopy; Minor; Molecular Analysis; Neoadjuvant Therapy; Neoplasm Metastasis; Noninfiltrating Intraductal Carcinoma; Patients; Proctitis; Radiation; Reproducibility; Research; Research Personnel; Resolution; Risk Marker; Scanning; Slide; Speed; Structure; Tissues; United States National Institutes of Health; Validation; Woman; cancer cell; chemotherapy; college; equipment acquisition; fluorescence imaging; imaging facilities; instrumentation; malignant breast neoplasm; mouse model; translational medicine; tumor; tumor microenvironment

PROJECT SUMMARY/ABSTRACT This application is an instrumentation grant from the Analytical Imaging Facility (AIF) at the Albert Einstein College of Medicine to acquire a fully automated, high-capacity, high-resolution slide scanner that can accommodate both brightfield and fluorescence imaging. The AIF of the Albert Einstein College of Medicine supports NIH-funded investigators by giving them access to state-of-the-art microscopy technologies that enhance collaborative, multidisciplinary research. Acquisition of this instrument will have a high impact on the biomedical research at Einstein and will expand the scope of NIH-funded projects at Einstein. Several projects have been identified that will heavily utilize the scanner. These include: the study of the formation of invasion promoting structures used to systemically disseminate metastatic cancer cells in carcinoma (Condeelis), correlative Intravital-Histopathologic Imaging to dissect the tumor microenvironment (Entenberg), establishment and validation of an immunocompromised radiation proctitis mouse model (Guha), studying the prometastatic effects of neoadjuvant chemotherapy in breast cancer (Karagiannis), investigating the effect of tumor environment on metastasis (Oktay), the analysis of molecular markers of risk of subsequent invasive breast cancer in women with ductal carcinoma in situ (Rohan), and the study of hepatocyte heterogeneity with respect to gene transcription signatures and their zonal distribution within the intact liver in healthy tissue as well as diseased patient tissue (Singer).There are also additional projects of Minor Users and many users in the Einstein community are anticipated. All of the projects in this application are in need of high-capacity and high- reproducibility scanning so that analyses may be applied to larger tissues, covering hundreds of fields of view, rather than the single fields of view acquired on a standard microscope.

项目总结/摘要 这个应用程序是一个仪器补助金从分析成像设施（AIF）在阿尔伯特爱因斯坦 医学院获得一个全自动，高容量，高分辨率的幻灯片扫描仪，可以 适应明场和荧光成像。阿尔伯特·爱因斯坦医学院AIF 支持NIH资助的研究人员，让他们获得最先进的显微镜技术， 加强多学科合作研究。购置这一仪器将对 生物医学研究在爱因斯坦，并将扩大NIH资助的项目在爱因斯坦的范围。几个项目 将大量使用扫描仪。其中包括：入侵形成的研究 促进用于在癌中全身性扩散转移性癌细胞的结构（Condeelis）， 相关的活体内组织病理学成像，以解剖肿瘤微环境（Entenberg），建立 和免疫受损放射性直肠炎小鼠模型（Guha）的验证，研究促转移性 新辅助化疗在乳腺癌（Karagiannis）中的作用，研究肿瘤 环境对转移的影响（Oktay），分析后续浸润性乳腺癌风险的分子标志物 乳腺导管原位癌（罗汉）妇女的癌症，以及肝细胞异质性的研究， 基因转录特征及其在健康组织中完整肝脏内的带状分布，以及 患病患者组织（Singer）。在Einstein中也有未成年人用户和许多用户的其他项目 社区期待。本申请中的所有项目都需要高容量和高- 重复性扫描，以便分析可以应用于更大的组织，覆盖数百个视场， 而不是在标准显微镜上获得的单个视场。