Automated Compound Storage and Retrieval System

自动化化合物存储和检索系统

• 批准号: 10415712
• 负责人: Joshua A. Bauer
• 金额: $ 74.14万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415712
• 关键词: Air; Bar Codes; Biology; Biotechnology; Categories; Chemicals; Collection; Communities; Effectiveness; Ensure; Environment; Equipment; Fee-for-Service Plans; Funding; Growth; Individual; Information Management; Institution; Laboratories; Libraries; Life Expectancy; Management Information Systems; Mission; Modeling; Molecular Bank; Pharmaceutical Preparations; Pharmacologic Substance; Research; Research Personnel; Retrieval; Sampling; Services; Site; Source; Speed; System; Temperature; Time; Translational Research; Tube; United States National Institutes of Health; Universities; Update; Vendor; Vial device; application programming interface; clinically relevant; drug discovery; high throughput screening; lead optimization; pre-clinical; programs; screening; screening services; tool

PROJECT SUMMARY/ABSTRACT Vanderbilt University is requesting funds for the purchase of a Hamilton Verso M2 to replace our obsolete Brooks Universal Store (Unistore). The current system was purchased in 2008 through funding from the NIH's Molecular Library Screening Center Network (MLSCN) and has since provided compound management services to Vanderbilt investigators performing chemical biology research. The Unistore is a cornerstone of the Vanderbilt High-throughput Screening (VHTS) Core providing a long-term storage environment, rapid retrieval, and tracking mechanism for each compound sample, whether in a tube or multi-well plate. This has enabled our institution to operate on par with biotech and pharmaceutical companies in terms of quality and effectiveness. The VHTS Unistore holds approximately 200,000 0.7mL tubes, 2,500 4-mL vials, and 7,000 384-well plates - totaling over 2.5 million samples. These samples are categorized as large, chemically diverse screening collections, libraries of known biologically active compounds, and project-specific compounds. The former two represent the majority of the samples and are available to any Vanderbilt researcher as a fee-for-service distribution. This service dovetails to the HTS screening services that span early discovery (e.g. tool compound discovery) and translational (e.g. discovery of new clinically-relevant activity of known drugs) projects. The third category (project-specific compounds) are compounds associated with specific, investigator-driven hits-to-leads and lead optimization preclinical drug discovery projects. Currently, samples from 111 such projects are managed through our system. Over the past five years, an average of 20,170 individual samples and 1,064 compound plate replications per year were distributed to investigators. The ability to quickly, efficiently and effectively support the demands of the Vanderbilt research community critically depends on our automated, environmentally controlled compound storage and retrieval system with integrated laboratory information management (LIMS) system. Although the Unistore has been an outstanding piece of equipment, it is well past its life expectancy and requires extensive on-site vendor support. The availability of vendor support is increasingly limited as Brooks introduces newer models. Replacement of the system is crucial to ongoing NIH-funded, as well as emerging, research programs aimed at advancing basic and early translational research. We are requesting funds for a new automated compound storage and retrieval system that will serve Vanderbilt through the next decade. Mission critical features that the Verso will provide include 1) 2-D barcode level tracking of tube samples, 2) 1-D barcode level tracking of plate samples, 3) environmental control with a dry air source and -20 °C redundant compressor temperature control, 4) high-speed tube picking, 5) storage capacity library growth, and 6) an application program interface (API) allowing streamlined integration with the existing VHTS LIMS. The timely replacement of the obsolete Brooks system with the updated Verso will ensure continued service to presently funded projects while providing a means to grow this capability within Vanderbilt.

项目概要/摘要 范德比尔特大学正在申请资金购买 Hamilton Verso M2 以取代我们过时的 Brooks 环球商店（Unistore）。目前的系统是 2008 年通过 NIH 分子生物学研究所的资助购买的。 图书馆筛选中心网络 (MLSCN)，此后为 范德比尔特研究人员进行化学生物学研究。 Unistore 是范德比尔特大学的基石 高通量筛选 (VHTS) 核心提供长期存储环境、快速检索和跟踪 每种化合物样品的机制，无论是在试管中还是在多孔板中。这使得我们的机构能够 在质量和有效性方面与生物技术和制药公司相当。甚高频传输系统 Unistore 拥有大约 200,000 个 0.7mL 试管、2,500 个 4mL 小瓶和 7,000 个 384 孔板 - 总计超过 250 万个样本。这些样本被归类为大型、化学多样化的筛选集合、文库 已知的生物活性化合物和项目特定的化合物。前两者占大多数 样本，并以按服务付费的方式提供给任何范德比尔特研究人员。这项服务 与涵盖早期发现（例如工具化合物发现）的 HTS 筛选服务相吻合 转化（例如发现已知药物的新的临床相关活性）项目。第三类 （项目特定化合物）是与特定的、研究者驱动的线索命中和线索相关的化合物 优化临床前药物发现项目。目前，111个此类项目的样本通过 我们的系统。过去五年，平均 20,170 个个体样品和 1,064 个复合板 每年的复制品分发给研究人员。能够快速、高效、有效地支持 范德比尔特研究界的需求很大程度上取决于我们的自动化、环境控制 具有集成实验室信息管理（LIMS）系统的化合物存储和检索系统。 尽管 Unistore 是一款出色的设备，但它已经远远超过了预期寿命，需要 广泛的现场供应商支持。随着布鲁克斯的推出，供应商支持的可用性越来越有限 较新的型号。更换系统对于 NIH 资助的持续研究以及新兴研究至关重要 旨在推进基础和早期转化研究的计划。我们正在为新项目申请资金 自动化复合存储和检索系统将在未来十年为范德比尔特提供服务。使命 Verso 将提供的关键功能包括 1) 管样品的二维条形码水平跟踪，2) 一维条形码 板样品的液位跟踪，3) 使用干燥空气源和 -20 °C 冗余压缩机进行环境控制 温度控制，4）高速试管拾取，5）存储容量库增长，6）应用程序 接口 (API)，允许与现有 VHTS LIMS 进行简化集成。及时更换 过时的 Brooks 系统与更新的 Verso 将确保继续为目前资助的项目提供服务，同时 提供一种在范德比尔特内部发展这种能力的方法。