Hypoxic incubator for physiological cell culture research

总结 该提案要求提供资金，以获得一个低氧培养箱和工作站在一个，这提供了精确的 温度、湿度、氧气和二氧化碳水平的控制。氧气含量可控制在0.1% 增量为0.1%至20%，因此涵盖了组织中发现的各种水平，包括 通常极度缺氧，氧含量在0.3%到2.0%之间。该应用程序将直接 支持6名NIH资助的研究人员和3名正在申请NIH资助的年轻研究人员。这一切成功都 研究人员有特定的项目，受益于HypOxystation® H35工作站的独特方面。 细胞在低氧张力下积累并激活低氧诱导转录因子α- HIF-1a和HIF-2a亚基，导致多效性作用，包括调节细胞代谢、细胞周期 和抗炎活性。这一工具的可用性将使关键的调查的作用， 低氧在调节辐射的免疫原性作用、癌症和糖尿病中的抗原呈递，以及 自身免疫中的线粒体功能障碍。HypOxystation® H35的独特功能，包括 工作空间、样品输入/输出端口、套筒端口和精确的环境控制和监测 培养和操作样品的稳定大气条件，这是进行严格的 研究氧张力在生物系统中的作用。HypOxystation® H35将提供 威尔康奈尔医学和邻近机构的能力，并将由一个顾问监督 委员会，由放射肿瘤科监督，因此将积极影响基本和 转化NIH资助的研究。由NIH资助的研究项目得到了这种仪器的支持， 与癌症生物学和治疗、免疫学、代谢和自身免疫相关。

SUMMARY This proposal requests funds to acquire a hypoxic incubator and workstation in one, which provides precise control of temperature, humidity, oxygen and carbon dioxide levels. Oxygen levels can be controlled in 0.1% increments from 0.1% to 20%, thus encompassing the various levels found in tissues, including in tumors which often are extremely hypoxic with levels ranging between 0.3 and 2.0% oxygen. This application will directly support 6 NIH-funded investigators and 3 young investigators currently applying for NIH funding. Each of these investigators has specific projects that benefits from the unique aspects of the HypOxystation® H35 workstation. Cells that are under low oxygen tension accumulate and activate hypoxia-inducible transcription factor alpha- subunits HIF-1a and HIF-2a, leading to pleiotropic effects including modulating cellular metabolism, cell cycle and anti-inflammatory activities. Availability of this instrument will enable crucial investigations of the role of hypoxia in modulating the immunogenic effects of radiation, antigen presentation in cancer and diabetes, and mitochondrial dysfunction in autoimmunity. Unique features of the HypOxystation® H35, including a large workspace, sample input/output port, sleeve ports and precise environmental control and monitoring produce stable atmospheric conditions for culturing and manipulating samples, which is required for carrying out rigorous research into the role of oxygen tension within a biological system. The HypOxystation® H35 will provide capabilities to Weill Cornell Medicine and neighboring institutions and will be supervised by an advisory committee, overseen by the Radiation Oncology department, and will thus positively impact basic and translational NIH-funded research. The NIH-funded research programs supported by this instrumentation have relevance to cancer biology and treatment, immunology, metabolism, and autoimmunity.