Hypoxic incubator for physiological cell culture research

用于生理细胞培养研究的低氧培养箱

• 批准号: 10427609
• 负责人: Sandra Demaria
• 金额: $ 10.45万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2023-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10427609
• 关键词: Advisory Committees; Anti-Inflammatory Agents; Antigen Presentation; Autoimmunity; Cancer Biology; Carbon Dioxide; Cell Culture Techniques; Cell Cycle; Cells; Diabetes Mellitus; Funding; Humidity; Hypoxia; Immunology; Incubators; Institution; Investigation; Malignant Neoplasms; Medicine; Metabolism; Monitor; Output; Oxygen; Physiological; Radiation Oncology; Request for Proposals; Research; Research Personnel; Role; Sampling; Supervision; Temperature; Tissues; United States National Institutes of Health; atmospheric conditions; biological systems; cancer therapy; immunogenic; instrument; instrumentation; mitochondrial dysfunction; pleiotropism; programs; radiation effect; transcription factor; tumor

SUMMARY This proposal requests funds to acquire a hypoxic incubator and workstation in one, which provides precise control of temperature, humidity, oxygen and carbon dioxide levels. Oxygen levels can be controlled in 0.1% increments from 0.1% to 20%, thus encompassing the various levels found in tissues, including in tumors which often are extremely hypoxic with levels ranging between 0.3 and 2.0% oxygen. This application will directly support 6 NIH-funded investigators and 3 young investigators currently applying for NIH funding. Each of these investigators has specific projects that benefits from the unique aspects of the HypOxystation® H35 workstation. Cells that are under low oxygen tension accumulate and activate hypoxia-inducible transcription factor alpha- subunits HIF-1a and HIF-2a, leading to pleiotropic effects including modulating cellular metabolism, cell cycle and anti-inflammatory activities. Availability of this instrument will enable crucial investigations of the role of hypoxia in modulating the immunogenic effects of radiation, antigen presentation in cancer and diabetes, and mitochondrial dysfunction in autoimmunity. Unique features of the HypOxystation® H35, including a large workspace, sample input/output port, sleeve ports and precise environmental control and monitoring produce stable atmospheric conditions for culturing and manipulating samples, which is required for carrying out rigorous research into the role of oxygen tension within a biological system. The HypOxystation® H35 will provide capabilities to Weill Cornell Medicine and neighboring institutions and will be supervised by an advisory committee, overseen by the Radiation Oncology department, and will thus positively impact basic and translational NIH-funded research. The NIH-funded research programs supported by this instrumentation have relevance to cancer biology and treatment, immunology, metabolism, and autoimmunity.

概括 该提案要求资金以一项获得缺氧的孵化器和工作站，以提供精确的 控制温度，湿度，氧和二氧化碳水平。氧气水平可以以0.1％的速度控制 从0.1％增加到20％，因此包括组织中发现的各种水平，包括在肿瘤中 通常非常低氧，水平在0.3至2.0％之间。此申请将直接 支持6名NIH资助的调查人员和3名目前申请NIH资金的年轻调查员。每个 研究人员拥有特定的项目，这些项目受益于HypoxyStation®H35工作站的独特方面。 低氧张力下的细胞会累积并激活低氧诱导的转录因子α- 亚基HIF-1A和HIF-2A，导致多效效应，包括调节细胞代谢，细胞周期 和抗炎活动。该工具的可用性将使至关重要的投资 在调节辐射的免疫原性，癌症和糖尿病中的抗原表现以及 自身免疫性的线粒体功能障碍。 HypoxyStation®H35的独特功能，包括大型 工作区，样品输入/输出端口，套筒端口以及精确的环境控制和监视生产 稳定的大气条件，用于聚类和操纵样品，这是进行严格进行的 研究氧张力在生物系统中的作用。 HypoxyStation®H35将提供 Weill Cornell医学和邻近机构的能力，将由咨询 受辐射肿瘤学部监督的委员会，因此将对基本和 转化NIH资助的研究。该工具支持的NIH资助的研究计划 与癌症生物学和治疗，免疫学，代谢和自身免疫有关。