Hypoxic incubator for physiological cell culture research

摘要 该提案要求获得资金，以购买集低氧孵化器和工作站于一体的设备，从而提供精确的 控制温度、湿度、氧气和二氧化碳水平。氧气水平可控制在0.1% 从0.1%到20%的增量，因此包括在组织中发现的各种水平，包括在 通常是极度缺氧，氧气含量在0.3%到2.0%之间。此应用程序将直接 支持6名NIH资助的研究人员和3名正在申请NIH资助的年轻研究人员。这其中的每一个 调查人员的特定项目受益于HypOxystation®H35工作站的独特方面。 处于低氧压下的细胞积聚并激活低氧诱导转录因子α- HIF-1a和HIF-2a亚基，导致多效性作用，包括调节细胞代谢，细胞周期 和抗炎活性。这一工具的提供将使关键的调查成为可能 低氧在调节辐射、癌症和糖尿病的抗原递呈的免疫原性效应中的作用，以及 自身免疫中的线粒体功能障碍。HypOxystation®H35的独特功能，包括大型 工作空间、样品输入/输出端口、套筒端口和精确的环境控制和监测产品 用于培养和处理样品的稳定的大气条件，这是执行严格的 研究氧张力在生物系统中的作用。HypOxystation®H35将提供 威尔·康奈尔医学和邻近机构的能力，并将由一名顾问监督 委员会，由放射肿瘤科监督，因此将对基本和 美国国立卫生研究院资助的翻译研究。由该仪器支持的美国国立卫生研究院资助的研究项目 与癌症生物学和治疗、免疫学、新陈代谢和自身免疫的相关性。

SUMMARY This proposal requests funds to acquire a hypoxic incubator and workstation in one, which provides precise control of temperature, humidity, oxygen and carbon dioxide levels. Oxygen levels can be controlled in 0.1% increments from 0.1% to 20%, thus encompassing the various levels found in tissues, including in tumors which often are extremely hypoxic with levels ranging between 0.3 and 2.0% oxygen. This application will directly support 6 NIH-funded investigators and 3 young investigators currently applying for NIH funding. Each of these investigators has specific projects that benefits from the unique aspects of the HypOxystation® H35 workstation. Cells that are under low oxygen tension accumulate and activate hypoxia-inducible transcription factor alpha- subunits HIF-1a and HIF-2a, leading to pleiotropic effects including modulating cellular metabolism, cell cycle and anti-inflammatory activities. Availability of this instrument will enable crucial investigations of the role of hypoxia in modulating the immunogenic effects of radiation, antigen presentation in cancer and diabetes, and mitochondrial dysfunction in autoimmunity. Unique features of the HypOxystation® H35, including a large workspace, sample input/output port, sleeve ports and precise environmental control and monitoring produce stable atmospheric conditions for culturing and manipulating samples, which is required for carrying out rigorous research into the role of oxygen tension within a biological system. The HypOxystation® H35 will provide capabilities to Weill Cornell Medicine and neighboring institutions and will be supervised by an advisory committee, overseen by the Radiation Oncology department, and will thus positively impact basic and translational NIH-funded research. The NIH-funded research programs supported by this instrumentation have relevance to cancer biology and treatment, immunology, metabolism, and autoimmunity.