Harnessing PET to Study the In Vivo Fate and Health Effects of Micro- and Nanoplastics

利用 PET 研究微塑料和纳米塑料的体内命运和健康影响

• 批准号: 10426972
• 负责人: Outi Maria Keinaenen
• 金额: $ 11.12万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-25 至 2023-08-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426972
• 关键词: Air; Animals; Autoradiography; Behavior; Biodistribution; Biological; Biological Markers; Biological Models; Cardiovascular system; Chelating Agents; Chronic; Communication; Consumption; Data; Decision Making; Deposition; Disease; Dose; Employment; Environment; Environmental Pollutants; Environmental Pollution; Evaluation; Exposure to; Fetal Development; Food; Foundations; Fresh Water; Future; Goals; Grant; Health; Histology; Human; Image; Imaging Techniques; Immunohistochemistry; In Vitro; Inflammation; Inflammatory; Inflammatory Response; Ingestion; Inhalation; Interdisciplinary Study; Intestines; Investigation; Iodine Radioisotopes; Label; Laboratories; Light; Liquid substance; Lung; Mammals; Mentors; Methods; Microscopic; Modeling; Mus; Musculoskeletal System; Nervous system structure; Nulliparity; Oral; Outcome; Particle Size; Pharmacology Study; Pharmacology and Toxicology; Phase; Physiological; Placenta; Plastics; Polystyrenes; Positron-Emission Tomography; Postdoctoral Fellow; Pregnancy; Process; Radiochemistry; Radiolabeled; Radiopharmaceuticals; Research; Resources; Role; Route; Seawater; Sewage; Shapes; Site; Soil; Stomach; Structure; Surface; Techniques; Time; Tissues; Toxic effect; Tracer; Visualization; Work; absorption; bioaccumulation; career; career development; collaborative environment; college; drinking water; experimental study; exposed human population; exposure route; imaging approach; in vivo; in vivo evaluation; innovation; molecular imaging; nuclear imaging; particle; pollutant; pre-clinical; pregnant; programs; radiotracer; remediation; research and development; sialic acid binding Ig-like lectin; skills; tool; trend; uptake

ABSTRACT CANDIDATE: The candidate is a postdoctoral fellow in the laboratory of Dr. Zeglis at Hunter College of CUNY. Her proposed research will provide a strong foundation for independent research career following the K99 phase of this grant. Her long-term career goal is to establish a robust program in environmental pollution research utilizing molecular imaging. To achieve this, she has developed a career plan that will (1) broaden her technical and scientific skills (2) strengthen her presentation and communication skills, and cultivate her professional relationships, (3) enrich her mentoring skills, and (4) prepare her for the transition to independence. RESEARCH: The proliferation of micro- and nanoplastics in the environment is increasing at an alarming rate. Micro- and nanoplastics have been found to be persistent and ubiquitous pollutants in a variety of environments, including sea water, fresh water, soil, sewage, and air. In light of this harrowing phenomenon, the evaluation of the possible health effects of microplastic (<5 mm) and nanoplastic (<100 nm) pollutants has emerged as an unmet scientific need. The over-arching goal of this investigation is to study the biodistribution and health effects of micro- and nanoplastics in mammals using a sensitive and quantitative molecular imaging technique: positron emission tomography (PET). The exquisite sensitivity of PET is major advantage of this strategy, as it will facilitate the non-invasive tracking of micro- and nanoplastics at environmentally relevant concentrations. The specific aims are: 1) To determine the bioaccumulation and bioretention of radioplastics in mice after pulmonary and oral exposure, 2) To quantify the bioaccumulation and bioretention of radioplastics in pregnant mice after oral and pulmonary exposure, and 3) To quantify the pro-inflammatory effects of micro- and nanoplastic exposure via non-invasive approaches. ENVIRONMENT: Hunter College is known for its highly collaborative environment to facilitate interdisciplinary research efforts such as those proposed in this application. The candidate is mentored by Dr. Zeglis, a recognized inorganic radiochemist with groundbreaking work in preclinical investigations of radiopharmaceuticals. In addition, she is co-mentored by Dr. Stapleton, an environmental toxicologist who has done pioneering and innovative contributions to the plastic research. Hunter College provides a rich set of collaborative, technical and scientific resources to execute the research and career development proposed here.

摘要 候选人：候选人是纽约市立大学亨特学院泽格里斯博士实验室的博士后研究员。 她提出的研究将为K99阶段之后的独立研究生涯奠定坚实的基础 这份赠款。她的长期职业目标是建立一个强大的环境污染研究计划 利用分子成像。为了实现这一目标，她制定了一个职业规划，将（1）扩大她的技术 （2）加强表达和沟通能力，培养专业能力 关系，（3）丰富她的指导技能，（4）为她过渡到独立做好准备。 研究：微塑料和纳米塑料在环境中的扩散正在以惊人的速度增加。 微塑料和纳米塑料已被发现是各种环境中的持久性和普遍存在的污染物， 包括海水、淡水、土壤、污水和空气。鉴于这一令人痛心的现象， 微塑料（<5 mm）和纳米塑料（<100 nm）污染物可能对健康造成的影响已经成为一个挑战， 未满足的科学需求本次调查的主要目的是研究其生物分布和健康效应 使用灵敏和定量的分子成像技术：正电子 发射断层扫描（PET）。PET的灵敏度是这种策略的主要优势，因为它将 促进在环境相关浓度下对微塑料和纳米塑料的非侵入性跟踪。的 具体目的是：1）确定肺内给药后放射性塑料在小鼠体内的生物蓄积和生物滞留 和经口暴露，2）定量放射性塑料在妊娠小鼠中的生物蓄积和生物滞留， 口腔和肺部暴露，以及3）量化微塑料和纳米塑料暴露的促炎作用 通过非侵入性方法。 环境：亨特学院以其高度协作的环境而闻名， 如本申请中提出的那些研究努力。候选人由Zeglis博士指导， 公认的无机放射化学家，在临床前研究方面具有开创性工作， 放射性药物此外，她还与环境毒理学家斯台普顿博士共同指导， 为塑料研究做出了开拓性和创新性的贡献。亨特学院提供丰富的 合作，技术和科学资源，以执行这里提出的研究和职业发展。