Diagnostics & Laboratory Core

诊断和实验室核心(核心D) 为了实施针对疾病和患者的护理，准确和快速地诊断儿科癌症至关重要。 核心包括乌干达和马拉维的临床实验室，这些实验室将提供组织学审查， 免疫组织化学(IHC)和流式细胞术。要监控的实验室测试 根据临床护理的需要，患者(CBCs、化学)也将在这些实验室进行。 将进行增强，以测试在多个 为在非洲建立一个更大的LMIC网络做准备，并实施虚拟病理学用于中央审查。 对于大型项目，集中病理检查，特别是对疑难病例，是势在必行的。 例如该联盟提出的建议。由于无法获得免疫组织化学(IHC)和 细胞组织学检查，NHL的错误分类在SSA中很常见。这在艾滋病病毒中更为关键。 NHL临床和病理特征不典型的情况，例如高级别B细胞性NHL DLBCL和BL之间的中间体被认为更普遍。此外，非皮肤性肺炎病例 卡波西肉瘤(KS)很难诊断。此核心提供的病理服务，以及 在资助期内对算法所做的改进将极大地增强 更准确地诊断儿童癌症在LMICs中的诊断。 该核心还将为研究研究提供样本处理、储存和运输的监督 包括在提案中。根据我们的经验，生物标本的集中处理确保了它们是 根据适当的标准化协议处理，从而最大限度地减少不必要的变异性 随后对样品进行分析。诊断和实验室核心(核心D)的职责包括 支持处理项目产生的所有生物检疫剂，并执行 SSA站点儿科癌症诊断算法的改进 为了实现这些目标，诊断和实验室核心的目标是： 1.协助适当地收集、加工、储存和运输所有生物杀虫剂 2.常规组织学复查、免疫组织化学和流式细胞术。 用于临床诊断和实验室检测的病例，用于持续监测患者 3.支持实施、培训和协调中央病理审查，利用虚拟 病理网络 4.通过支持现场验证和 关于其他方法的培训 5.协助发展核心(核心B)培训更多病理学家和化验室

Diagnostic & Laboratory Core (Core D) To administer disease- and patient-specific care, accurate and rapid diagnosis of pediatric cancer is critical. The core includes the clinical laboratories in Uganda and Malawi, which will provide histological review, immunohistochemistry (IHC), and flow cytometry for prospective patients. Laboratory testing to monitor patients (CBCs, chemistry) will also be performed in these laboratories, as required for clinical care. Enhancements will be made to test the feasibility of using standardized diagnostic algorithms across multiple sites and implementing virtual pathology for central review, in preparation for a larger LMIC network in Africa. Centralized pathology review, especially for difficult to diagnose cases, is imperative for large-scale projects, such as those proposed by the Consortium. Due to lack of access to immunohistochemistry (IHC) and cytohistological review, misclassification of NHLs is common in SSA. This is even more critical in the HIV setting where the clinical and pathology characteristics of NHL are atypical e.g. high-grade B-cell NHL intermediate between DLBCL and BL are thought to be more prevalent. Further, non-cutaneous cases of Kaposi's sarcoma (KS) can be difficult to diagnose. The pathology services offered by this Core, and the improvements made to the algorithm over the course of the funding period, will greatly enhance the ability to more precisely diagnosis pediatric cancer diagnosis in the LMICs. The core will also provide oversight of sample processing, storage, and shipping for the research studies included in the proposal. In our experience, centralized processing of biospecimens ensures that they are processed according to appropriate standardized protocols, which minimizes unwanted variability when the samples are later analyzed. The responsibilities of the Diagnostic & Laboratory Core (Core D) include support for the processing of all biospecimens generated from the Projects and for implementing improvements in the pediatric cancer diagnosis algorithm at the SSA sites To accomplish these objectives, the Aims of the Diagnostic & Laboratory Core are: 1. To assist with the appropriate collection, processing, storage, and shipping of all biospecimens 2. To perform routine histologic review, immunohistochemistry, and flow cytometry of pediatric cancer cases for clinical diagnosis and laboratory testing for the ongoing monitoring of patients 3. To support the implementation, training, and coordination of central pathology review utilizing a virtual pathology network 4. To implement enhancements to clinical diagnostic algorithms by supporting onsite validation and training on additional methodologies 5. To assist the Developmental Core (Core B) in the education of additional pathologists and laboratory