Pro-inflammatory activation of human macrophages regulated by lncRNAs

项目摘要 巨噬细胞的激活促进了包括动脉疾病在内的主要炎症性疾病。其潜在的原因 本研究将建立一个系统的方法，涉及 计算预测分析、多组学、网络科学以及体外和体内验证，以 发现长非编码RNA(LncRNA)介导的促炎激活机制 巨噬细胞与动脉疾病。在具体目标1中，我们将涉及对人巨噬细胞的组学研究。 识别lncRNAs及其相互作用的蛋白，并开发计算机分析来预测人类 调节巨噬细胞激活的lncRNA。具体目标2将检查候选人的功能 体内和体外巨噬细胞激活中的lncRNAs。这项研究的发现将有助于识别新的 巨噬细胞激活的机制，并可能为新的治疗方法提供分子基础。

Project Summary Macrophage activation promotes major inflammatory disorders, including arterial diseases. Its underlying mechanisms, however, remain obscure. The present study will establish a systems approach, involving computational prediction analyses, multi-omics, network science, and in vitro and in vivo validation, to discover long noncoding RNA (lncRNA)-mediated mechanisms for pro-inflammatory activation of macrophages and arterial disease. In Specific Aim 1, we will involve omics studies of human macrophages to identify lncRNAs and their interacting proteins and develop computational analyses to predict human lncRNAs that regulate macrophage activation. Specific Aim 2 will examine the functionality of candidate lncRNAs in macrophage activation in vitro and in vivo. The findings from the study will help to identify new mechanisms for macrophage activation and may provide molecular bases for new therapies.