Pro-inflammatory activation of human macrophages regulated by lncRNAs

lncRNA调控人巨噬细胞的促炎激活

• 批准号: 9973174
• 负责人: Masanori Aikawa
• 金额: $ 72.63万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9973174
• 关键词: Address; Biological; Biological Process; Biology; Blood; Blood Vessels; Cells; Communities; Complex; Computational Biology; Computer Analysis; Coronary Arteriosclerosis; Data; Development; Discipline; Disease; Drug usage; Endotoxemia; Event; Gene Expression Profiling; Goals; Hematopoietic Stem Cell Transplantation; Heterogeneity; Human; In Vitro; Inflammation; Inflammatory; Laboratories; Lesion; Leukocytes; Life; Link; Machine Learning; Macrophage Activation; Mechanics; Mediating; Methods; Molecular; Myocardial; NF-kappa B; Network-based; Outcome; Pathway Analysis; Patients; Plasma; Play; Protein Analysis; Proteins; Proteomics; RNA; Reporting; Residual state; Risk; Risk Factors; Role; Science; Signal Transduction; Small Interfering RNA; Splenocyte; System; Systems Biology; Tissues; Untranslated RNA; Validation; Vascular Diseases; arterial lesion; base; cytokine; experimental study; femoral artery; gain of function; global health; human disease; humanized mouse; in vivo; injured; loss of function; macrophage; modifiable risk; mouse model; multiple omics; network models; novel; novel therapeutics; overexpression; protein protein interaction; single cell analysis; tool; transcriptome; transcriptome sequencing; transcriptomics; vascular inflammation

Project Summary Macrophage activation promotes major inflammatory disorders, including arterial diseases. Its underlying mechanisms, however, remain obscure. The present study will establish a systems approach, involving computational prediction analyses, multi-omics, network science, and in vitro and in vivo validation, to discover long noncoding RNA (lncRNA)-mediated mechanisms for pro-inflammatory activation of macrophages and arterial disease. In Specific Aim 1, we will involve omics studies of human macrophages to identify lncRNAs and their interacting proteins and develop computational analyses to predict human lncRNAs that regulate macrophage activation. Specific Aim 2 will examine the functionality of candidate lncRNAs in macrophage activation in vitro and in vivo. The findings from the study will help to identify new mechanisms for macrophage activation and may provide molecular bases for new therapies.

项目总结