Sartorious Octet R8 System

赛多利斯八位位组 R8 系统

• 批准号: 10429632
• 负责人: Manish Sagar
• 金额: $ 24.91万
• 依托单位: BOSTON MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-17 至 2023-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429632
• 关键词: Advisory Committees; Antibodies; Autoimmune Diseases; Basic Science; Binding; Biological Assay; Boston; Cardiovascular Diseases; Clinical Research; Communicable Diseases; Communities; Diabetes Mellitus; Diagnosis; Education; Equipment; Event; Fc Receptor; Feedback; Financial Support; Funding; Goals; Grant; Heart Diseases; In Vitro; Individual; Industry; Institution; Interferometry; Investments; Kidney Diseases; Kinetics; Maintenance; Medical; Medical center; Mission; Modernization; Monitor; Obesity; Pathogenesis; Peptides; Price; Progress Reports; Proteins; Publications; Reporting; Research; Research Personnel; Scientist; Services; System; Time; Training; Translational Research; United States National Institutes of Health; Universities; Virus; base; cost; drug development; experience; human disease; instrument; instrumentation; novel; pandemic disease; programs; small molecule; targeted treatment; vaccine development

PROJECT SUMMARY / ABSTRACT The proposed S10 project entitled “Octet R8 System for Molecular Interaction Studies in Infectious and Non-infectious Diseases” is to respond to PAR-21-125 Basic Instrumentation Grant (BIG) Program. The mission of the Analytical Instrumentation Core (AIC) at Boston Medical Center (BMC) and Boston University (BU) is to provide support and training to investigators through cutting-edge analytical instruments and services to facilitate research and education. The ultimate goal is to contribute to the understanding of the pathogenesis, diagnosis, and treatment of a diverse range of human diseases. The AIC meets this goal by providing access to modern, sophisticated high-priced instruments that are necessary to advancing the research of multiple biomedical investigators. These instruments are typically cost-prohibitive for individual scientists to purchase, operate, and maintain. Based on feedback from our investigators, having highly sensitive approaches for identifying and quantifying intramolecular interactions between antibodies, receptors, peptides, small molecules, and viruses have consistently been reported as an AIC service of the greatest need. This is especially relevant because many groups have started more translational projects over the past several years (particularly because of the current pandemic) and require the ability to quantitatively detect binding between novel targets and therapeutics. This desire from multiple investigators has led us to explore the possibility of purchasing a Sartorius 8-channel Octet biolayer interferometry (BLI) R8 system. Industry- leading BLI analytical instruments precisely and accurately monitor binding between two different molecules in real-time. The Octet R8 will provide investigators a highly sensitive and reliable way to conduct binding assays, to quantify proteins, antibodies, peptides and small molecules, and to directly monitor the kinetics of binding events and interactions between biomolecules from in-vitro and ex-vivo studies. The AIC provides services to more than 150 investigators/labs, and has over 10 NIH funded projects that would directly utilize the Octet R8. Presence of BLI services will allow researchers to perform their projects with greater precision and sensitivity. We propose that the Octet-R8 will be housed at the AIC, which is located on the BU Medical Campus. However, it will still be equally accessible to investigators across the entire research community. This will be highly beneficial because the AIC has extensive experience and a magnificent record of accomplishment regarding instrument maintenance and training users. The instrument will be assured to be in excellent working condition, thus minimizing any potential down time. The institution will provide substantial financial support and there is also an advisory committee to assist with biosafety needs, publications, and annual progress reports. Investment in this equipment will allow our investigators to conduct studies of high significance for basic, translational and clinical research into infectious diseases, vaccine development, diabetes, heart disease, obesity, kidney disease, autoimmune diseases, cardiovascular diseases, and drug development.

项目总结/摘要 拟议的S10项目题为“用于传染病和结核病分子相互作用研究的Octet R8系统”， 非传染性疾病”是为了回应PAR-21-125基本仪器补助金（BIG）计划。的 波士顿医学中心（BMC）和波士顿大学的分析仪器核心（AIC）的使命 (BU)通过先进的分析仪器和服务为调查人员提供支持和培训 以促进研究和教育。最终目标是帮助理解 发病机理、诊断和治疗多种人类疾病。AIC通过以下方式实现这一目标： 提供现代化的、复杂的、昂贵的工具，这是推进 多个生物医学研究人员的研究。这些仪器对于个人来说通常成本过高 科学家购买，操作和维护。根据我们调查人员的反馈， 用于鉴定和定量抗体，受体， 肽，小分子和病毒一直被报道为最大的AIC服务。 需要的这一点尤其重要，因为许多小组在过去已经开始了更多的翻译项目 几年（特别是由于目前的流行病），并需要定量检测的能力， 新靶点和治疗剂之间的结合。多位研究者的这种愿望促使我们探索 购买Sartorius 8通道Octet生物层干涉测量（BLI）R8系统的可能性。工业- 领先的BLI分析仪器精确地监测两种不同分子之间的结合， 实时的Octet R8将为研究人员提供一种高度灵敏和可靠的方法来进行结合试验， 定量蛋白质、抗体、肽和小分子，并直接监测结合动力学 来自体外和离体研究的生物分子之间的事件和相互作用。AIC提供服务， 超过150名研究人员/实验室，并有超过10个NIH资助的项目将直接利用Octet R8。 BLI服务的存在将使研究人员能够以更高的精度和灵敏度执行他们的项目。 我们建议将Octet-R8安置在位于BU医学园区的AIC。 然而，整个研究界的研究人员仍然可以平等地访问它。这将是 非常有益，因为AIC拥有丰富的经验和辉煌的成就记录 关于仪器维护和培训用户。仪器将保证处于良好的工作状态 条件，从而最大限度地减少任何潜在的停机时间。该机构将提供大量财政支持， 还有一个咨询委员会，负责协助满足生物安全需要、编写出版物和年度进展报告。 对这一设备的投资将使我们的研究人员能够进行对基础， 传染病、疫苗开发、糖尿病、心脏病、 肥胖、肾脏疾病、自身免疫性疾病、心血管疾病和药物开发。