Sartorious Octet R8 System

项目摘要/摘要 建议的S10项目“用于感染性和非传染性疾病分子相互作用研究的八位组R8系统”。 非传染性疾病“是为了响应PAR-21-125基本仪器设备资助(BIG)计划。这个 波士顿医学中心(BMC)和波士顿大学分析仪器核心(AIC)的使命 (BU)是通过尖端的分析仪器和服务为调查人员提供支持和培训 以促进研究和教育。最终目标是促进对 多种人类疾病的发病机制、诊断和治疗。AIC通过以下方式实现这一目标 提供获得现代、复杂的高价工具的途径，这些工具对于推动 多个生物医学研究者的研究。这些工具对于个人来说通常是成本高昂的 科学家需要购买、操作和维护。根据我们调查人员的反馈，已经高度 用于识别和量化抗体、受体、 多肽、小分子和病毒一直被报道为最大的AIC服务 需要。这一点尤其重要，因为许多组织在过去开始了更多的翻译项目 几年(特别是因为目前的大流行)，并需要有能力定量检测 新靶点和治疗药物之间的结合。来自多个调查人员的这种愿望引导我们探索 购买Sartorius 8通道八位组生物干涉测量(BLI)R8系统的可能性。行业- 领先的BLI分析仪器精确和准确地监测两个不同分子之间的结合 实时的。Octet R8将为调查人员提供一种高度敏感和可靠的方法来进行绑定分析， 对蛋白质、抗体、多肽和小分子进行定量，并直接监测结合动力学 来自体外和体外研究的生物分子之间的事件和相互作用。AIC提供以下服务： 超过150名研究人员/实验室，并拥有10多个由NIH资助的项目，这些项目将直接利用Octet R8。 BLI服务的存在将使研究人员能够更精确和更敏感地执行他们的项目。 我们建议将OCTET-R8放置在位于布宜诺斯艾利斯大学医学院的AIC。 然而，整个研究界的调查人员仍然可以平等地访问它。这将是 非常有益，因为AIC拥有丰富的经验和辉煌的成就记录 关于仪器维护和培训用户。该仪器将被保证良好地工作 条件，从而最大限度地减少任何潜在的停机时间。该机构将提供大量财政支持，并 还有一个咨询委员会，协助解决生物安全需求、出版物和年度进展报告。 对这台设备的投资将使我们的研究人员能够对基础、 对传染病、疫苗开发、糖尿病、心脏病、 肥胖、肾脏疾病、自身免疫性疾病、心血管疾病和药物开发。

PROJECT SUMMARY / ABSTRACT The proposed S10 project entitled “Octet R8 System for Molecular Interaction Studies in Infectious and Non-infectious Diseases” is to respond to PAR-21-125 Basic Instrumentation Grant (BIG) Program. The mission of the Analytical Instrumentation Core (AIC) at Boston Medical Center (BMC) and Boston University (BU) is to provide support and training to investigators through cutting-edge analytical instruments and services to facilitate research and education. The ultimate goal is to contribute to the understanding of the pathogenesis, diagnosis, and treatment of a diverse range of human diseases. The AIC meets this goal by providing access to modern, sophisticated high-priced instruments that are necessary to advancing the research of multiple biomedical investigators. These instruments are typically cost-prohibitive for individual scientists to purchase, operate, and maintain. Based on feedback from our investigators, having highly sensitive approaches for identifying and quantifying intramolecular interactions between antibodies, receptors, peptides, small molecules, and viruses have consistently been reported as an AIC service of the greatest need. This is especially relevant because many groups have started more translational projects over the past several years (particularly because of the current pandemic) and require the ability to quantitatively detect binding between novel targets and therapeutics. This desire from multiple investigators has led us to explore the possibility of purchasing a Sartorius 8-channel Octet biolayer interferometry (BLI) R8 system. Industry- leading BLI analytical instruments precisely and accurately monitor binding between two different molecules in real-time. The Octet R8 will provide investigators a highly sensitive and reliable way to conduct binding assays, to quantify proteins, antibodies, peptides and small molecules, and to directly monitor the kinetics of binding events and interactions between biomolecules from in-vitro and ex-vivo studies. The AIC provides services to more than 150 investigators/labs, and has over 10 NIH funded projects that would directly utilize the Octet R8. Presence of BLI services will allow researchers to perform their projects with greater precision and sensitivity. We propose that the Octet-R8 will be housed at the AIC, which is located on the BU Medical Campus. However, it will still be equally accessible to investigators across the entire research community. This will be highly beneficial because the AIC has extensive experience and a magnificent record of accomplishment regarding instrument maintenance and training users. The instrument will be assured to be in excellent working condition, thus minimizing any potential down time. The institution will provide substantial financial support and there is also an advisory committee to assist with biosafety needs, publications, and annual progress reports. Investment in this equipment will allow our investigators to conduct studies of high significance for basic, translational and clinical research into infectious diseases, vaccine development, diabetes, heart disease, obesity, kidney disease, autoimmune diseases, cardiovascular diseases, and drug development.