Washington University Center for Cellular Imaging

华盛顿大学细胞成像中心(WUCCI)共享资源： 项目总结 阐明癌症发生和发展所涉及的机制需要一个深入的 了解组织/细胞结构的变化与功能丧失的关系。成像技术已经 在过去的十年中快速发展，在分辨率、灵敏度和速度方面产生了巨大的改进，并且 为研究多个数量级的生物过程创造了全新的机会 在活细胞和生物体中的实时。华盛顿大学细胞成像中心(WUCCI)提供 (1)可靠和负担得起的尖端光学和电子显微镜仪器，(2)专家 指导成像分析设计、标本制备、图像采集和分析；(Iii)教育 机会形式包括动手培训、午餐和学习讲座、研讨会和月刊俱乐部 (4)新的成像和分析方法。作为新的癌症中心支持赠款(CCSG)共享 资源(SR)，WUCCI将提供一种集成的方法来调查 癌症相关研究中的细胞和组织。通过利用重大的机构投资，SCC的研究人员 即时获得各种先进的细胞显微镜工具，这将有助于加速 加快研究步伐，扩大研究范围，提高研究效率。WUCCI服务满足独特的 众多研究人员对广泛的基础和翻译研究的要求，以及吸引 癌症研究领域的新调查人员。重要的是，用户从深入的技术专业知识中受益 主任(詹姆斯·菲茨帕特里克)和技术人员关于实验设计和解释 数据。在过去的三年里，WUCCI与多名SCC研究人员合作实施了新的成像 化验。其中包括应用双光子活体显微镜进行纵向追踪。 小鼠休眠模型中的播散性肿瘤细胞(Sheila Stewart[MCBP])，X射线的发展 用造影剂描绘小鼠长骨中转移的肿瘤边界(凯瑟琳·韦尔比彻) [BCRP])，以及聚焦离子束扫描电子显微镜(FIB-SEM)系列块面的应用 成像以获取纳米尺度的小鼠胃组织的三维体积(Jason Mills[MCBP])。 2018年，WUCCI为235个研究实验室提供服务，其中75个是SCC调查人员，他们的使用 约占SR总消耗量的20%。下一个项目期的未来计划包括收购 最近由NIH S10计划资助的用于高速体积测量的光片荧光显微镜 活体有机物和清除的组织的成像以及Visiopamoncotopix的实施 IHC和ISH图像分析平台。我们预计对WUCCI服务的需求将继续增长 鉴于在癌症研究中需要先进的显微成像和分析。因此，WUCCI作为一种新的 CCSG共享资源，将在下一个项目期间为SCC调查人员提供必要的服务。

WASHINGTON UNIVERSITY CENTER FOR CELLULAR IMAGING (WUCCI) SHARED RESOURCE: PROJECT SUMMARY Elucidating the mechanisms involved in the development and progression of cancer requires a deep understanding of how changes in tissue / cellular structure relate to loss of function. Imaging technologies have evolved rapidly over the last decade, generating vast improvements in resolution, sensitivity, and speed, and creating fundamentally new opportunities for studying biological processes across many orders of magnitude in real-time in living cells and organisms. The Washington University Center for Cellular Imaging (WUCCI) provides (i) reliable and affordable access to cutting-edge light and electron microscopy instrumentation, (ii) expert guidance in imaging assay design, specimen preparation, image acquisition and analysis, (iii) educational opportunities in the form of hands-on trainings, lunch and learn lectures, workshops and a monthly journal club and (iv) novel imaging and analysis methodologies. As a new Cancer Center Support Grant (CCSG) shared resource (SR), WUCCI will provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues in cancer-related studies. By leveraging a significant institutional investment, SCC researchers gain instant access to a wide variety of advanced cellular microscopy tools which will serve to accelerate the pace, expand the scope, and improve the efficiency of their research. WUCCI services meet the unique requirements of numerous investigators over a wide range of basic and translational research, as well as attract new investigators into the cancer research arena. Importantly, users benefit from the in-depth technical expertise of the Director (James Fitzpatrick) and technical staff with regard to experimental design and interpretation of data. Over the past three years, WUCCI has worked with multiple SCC researchers to implement new imaging assays. These have included the application of two-photon intravital microscopy to longitudinally track disseminated tumor cells in a mouse model of dormancy (Sheila Stewart [MCBP]), the development of x-ray contrast agents to delineate metastatic tumor boundaries in murine long bones (Katherine Weilbeacher [BCRP]), and the application of focused ion beam-scanning electron microscopy (FIB-SEM) serial block face imaging to acquire three-dimensional volumes of mouse gastric tissue at the nanoscale (Jason Mills [MCBP]). In 2018, WUCCI served 235 research laboratories, 75 of which were SCC investigators whose usage represented ~20% of the overall SR consumption. Future plans for the next project period include the acquisition of a light-sheet fluorescence microscope recently funded by the NIH S10 program for the high-speed volumetric imaging of living organoids and cleared tissues, as well as the implementation of the Visiopharm Oncotopix IHC and ISH image analysis platform. We anticipate that requests for WUCCI services will continue to grow given the need for advanced microscopic imaging and analysis in the study of cancer. Thus, WUCCI, as a new CCSG shared resource, will provide essential services to SCC investigators throughout the next project period.