Novel Multi-Depth Two-Photon Microscope for Measuring Neuronal Network Plasticity

项目总结 数十年来在人类受试者和动物模型中的研究表明，海马体是一个关键的 大脑结构既参与记忆的形成，也参与空间导航。这两种计算都是 被认为在我们的环境中创建了一张特征的认知地图，其中通常包含动态的感觉 随时间不断变化的功能。然而，目前尚不清楚这些看似独立的计算是否 在海马体内是相互依赖的，招募共同的电路，或利用类似的编码策略。在 家长R21开拓者奖，我们建议建造一种能够对神经元进行成像的新型显微镜 同时在多个海马区活动，以询问海马区是如何动态学习的 通过研究小鼠音乐记忆的形成来获得信息。在多样性副刊中，候选人 克里斯蒂安·夏普计划利用这项显微镜技术来研究海马体及其 包含动态感官信息的空间环境导航中的不同子域。 克里斯蒂安的目标是成为一名神经学家，致力于尖端神经技术和设计 严谨的实验来回答有关大脑功能的基本问题。作为当前多样性的一部分 我们已经制定了一份详细的职业发展计划，将促进克里斯蒂安从 从物理领域转到生物学领域，并通过与经验丰富的 多学科导师团队，其中包括马修·施特拉曼博士、布伦达·布拉德古德博士和大卫 Kleinfeld；2)遵循个性化学习计划，通过正式的学习掌握生物学的核心概念 课业；3)进行以假说为中心的生物学研究；4)学习与他人交流 通过科学讲座、手稿和拨款申请进行研究；5)参加专业发展 课程和研究会议；以及6)准备和提交NSF研究生研究奖学金 在授权期内申请。在NIH多样性副刊的支持下，通过实现这些 职业发展的里程碑，克里斯蒂安将为研究生院和他未来的研究生涯做好准备 在神经科学方面。

PROJECT SUMMARY Decades of studies in both human subjects and animal models indicate that the hippocampus is a critical brain structure involved in both the formation of memories and spatial navigation. Both of these computations are thought to create a cognitive map of features within our environment, which often contains dynamic sensory features that continuously change with time. However, it is not known if these seemingly separate computations within the hippocampus are interdependent, recruit common circuits, or utilize similar encoding strategies. In the parent R21 Trailblazer Award, we proposed to build a novel microscope that is capable of imaging neuronal activity in multiple hippocampal subfields simultaneously in order to ask how the hippocampus learns dynamic information by studying the formation of musical memories in mice. In the diversity supplement, the candidate Cristian Sharp plans to capitalize on this microscope technology to study the role of the hippocampus and its different subfields in navigation of spatial environments containing dynamic sensory information. Cristian’s goal is to become a neuroscientist, working on cutting edge neurotechnology and designing rigorous experiments to answer fundamental questions about brain function. As part of the current diversity supplement we have devised a detailed career development plan that will facilitate Cristian’s transition from the field of physics to biology and prepare him for graduate school in neuroscience by 1) working with an experienced multidisciplinary team of mentors, which include Drs. Matthew Shtrahman, Brenda Bloodgood, and David Kleinfeld; 2) following an individualized learning plan, mastering core concepts in biology through formal coursework; 3) performing hypothesis-focused biological research; 4) learning to communicate about his research through scientific lectures, manuscripts, and grant applications; 5) attending professional development courses and research conferences; and 6) preparing and submitting an NSF Graduate Research Fellowship application during the award period. With the support of the NIH diversity supplement and by achieving these career development milestones, Cristian will be well-poised for graduate school and his future research career in neuroscience.