Zeiss LSM 980 Confocal Microscope System

蔡司 LSM 980 共焦显微镜系统

• 批准号: 10431309
• 负责人: Alex M Valm
• 金额: $ 47.67万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431309
• 关键词: Afferent Neurons; Behavior; Biological Sciences; Capital; Cell Communication; Cell Differentiation process; Cells; Communities; Data; Development; Disease; Education; Extracellular Matrix; Faculty; Fluorescence Microscopy; GNRH1 gene; Gene Expression Regulation; Genetic Transcription; Health; Heat shock proteins; Homeostasis; Human; Image; Institutes; Institution; Microscope; Minor; Natural regeneration; Neurons; New York; Organ; Postdoctoral Fellow; Protein Biosynthesis; RNA; Regulation; Research; Resolution; Speed; Structure-Activity Relationship; System; Technology; Time; Transcriptional Activation; Translational Regulation; Universities; Virus Diseases; collaborative approach; daughter cell; epitranscriptomics; fluorescence microscope; graduate student; high resolution imaging; imaging platform; imaging system; microbial community; multidisciplinary; neuron development; oral microbiome; organ growth; programs; research facility; stem cell biology; stem cells; two-photon; undergraduate student

Project Summary/Abstract The Life Science Initiative and the RNA Institute of the State University of New York at Albany (UAlbany) represent a regional hub for multidisciplinary and collaborative research by undergraduate and graduate students, post-doctoral fellows, research staff, and faculty. This highly interactive institution operates on a dynamic collaborative approach toward scientific discoveries and high-quality education. To continue our faculty’s historic interdisciplinary collaborative research ventures through our shared core research facilities, we have identified an urgent need for a Zeiss LSM 980 confocal microscope system with the latest Airyscan 2 technology. This advanced quantitative fluorescence microscopy is capable of fast image acquisition with ultrahigh spatial resolution and multispectral imaging at high spectral resolution. Acquisition of this advanced imaging platform will facilitate a wide variety of research programs across our major and minor user groups encompassing diverse fields including stem cell biology; cell-cell communication in organ development; neuronal development and behavior; microbial communities in human health; gene regulation and transcriptional activation; epitranscriptomic regulation by viral infection, environmental, and physical stress; protein biosynthesis and translational regulation. The most advanced imaging systems currently available at UAlbany, the conventional confocal Zeiss LSM710 fluorescence microscopy and two-photon fluorescence microscope, lack sufficient resolution, sensitivity and speed to support these multidisciplinary cutting-edge research programs. Our major users, in particular, require the Zeiss 980 superior resolution and imaging capacity to answer critical research questions that are central to their research programs, including: 1) understanding the interactions between niche cells and stem cell daughters responsible for cell differentiation; 2) defining the cell-cell and cell-ECM interactions that regulate organ-formation and regeneration in disease; 3) understanding the development and regeneration of sensory neurons and GnRH-1 neurons in homeostasis and disease, and 4) defining systems level structure-function relationships in the oral microbiome. The imaging system is also critical to support the diverse research programs of our many minor users to acquire multispectral data, high resolution images, and fast time-lapse images to answer critical research questions. The excellent upgrade capabilities of this system will enable our program’s major and minor users as well as the broader research community of New York’s capital region to meet the imaging research needs of their cutting-edge research programs.

项目概要/摘要 生命科学计划和纽约州立大学奥尔巴尼分校 (UAlbany) 的 RNA 研究所 代表本科生和研究生多学科和协作研究的区域中心 学生、博士后研究员、研究人员和教师。这个高度互动的机构运作于 实现科学发现和高质量教育的动态协作方法。继续我们的 教师通过我们共享的核心研究设施进行历史性的跨学科合作研究， 我们发现迫切需要配备最新 Airyscan 2 的 Zeiss LSM 980 共焦显微镜系统 技术。这种先进的定量荧光显微镜能够快速采集图像 超高空间分辨率和高光谱分辨率下的多光谱成像。获得这一先进的 成像平台将促进我们主要和次要用户群体的各种研究计划 涵盖不同领域，包括干细胞生物学；器官发育中的细胞间通讯； 神经元发育和行为；微生物群落对人类健康的影响；基因调控和 转录激活；病毒感染、环境和身体压力的表观转录调控； 蛋白质生物合成和翻译调控。目前最先进的成像系统 UAlbany，传统共焦蔡司LSM710荧光显微镜和双光子荧光 显微镜缺乏足够的分辨率、灵敏度和速度来支持这些多学科的尖端技术 研究计划。我们的主要用户尤其需要 Zeiss 980 卓越的分辨率和成像 回答对其研究项目至关重要的关键研究问题的能力，包括：1) 了解微环境细胞和负责细胞分化的干细胞子细胞之间的相互作用； 2）定义调节疾病中器官形成和再生的细胞-细胞和细胞-ECM相互作用； 3） 了解感觉神经元和 GnRH-1 神经元在稳态中的发育和再生 和疾病，4) 定义口腔微生物组中的系统级结构-功能关系。成像 系统对于支持我们许多未成年用户的多样化研究计划也至关重要 多光谱数据、高分辨率图像和快速延时图像来回答关键的研究问题。 该系统出色的升级能力将使我们程序的主要用户和次要用户以及 纽约首都地区更广泛的研究界，以满足他们的成像研究需求 尖端研究计划。