Zeiss LSM 980 Confocal Microscope System

项目概要/摘要 位于奥尔巴尼的纽约州立大学生命科学倡议和RNA研究所（奥尔巴尼大学） 代表本科生和研究生多学科和合作研究的区域中心 学生，博士后研究员，研究人员和教师。这个高度互动的机构在一个 动态协作的方法对科学发现和高质量的教育。继续我们 教师的历史性跨学科合作研究企业通过我们的共享核心研究设施， 我们已经确定迫切需要一个蔡司LSM 980共聚焦显微镜系统与最新的Airyscan 2 技术.这种先进的定量荧光显微镜能够快速采集图像， 空间分辨率和高光谱分辨率下的多光谱成像。收购这一先进的 成像平台将促进我们的主要和次要用户群体的各种研究计划 包括干细胞生物学、器官发育中的细胞间通讯、 神经发育和行为;人类健康中的微生物群落;基因调控和 转录激活;由病毒感染、环境和物理应激引起的表观转录调控; 蛋白质生物合成和翻译调控。目前最先进的成像系统， UAlbany，常规共焦Zeiss LSM 710荧光显微镜和双光子荧光 显微镜，缺乏足够的分辨率，灵敏度和速度，以支持这些多学科的尖端 研究项目。我们的主要用户尤其需要蔡司980上级分辨率和成像 回答关键研究问题的能力，这些问题是他们研究计划的核心，包括：1） 理解小生境细胞和负责细胞分化的干细胞子细胞之间的相互作用; 2)定义在疾病中调节器官形成和再生的细胞-细胞和细胞-ECM相互作用; 3） 了解感觉神经元和GnRH-1神经元在稳态中的发育和再生 和疾病，以及4）定义口腔微生物组中的系统水平结构-功能关系。成像 系统也是至关重要的，以支持我们的许多未成年人用户的各种研究计划，以获得 多光谱数据、高分辨率图像和快速延时图像来回答关键的研究问题。 该系统的优秀升级能力将使我们的程序的主要和次要用户以及 纽约首都地区更广泛的研究社区，以满足他们的成像研究需求， 尖端研究项目

Project Summary/Abstract The Life Science Initiative and the RNA Institute of the State University of New York at Albany (UAlbany) represent a regional hub for multidisciplinary and collaborative research by undergraduate and graduate students, post-doctoral fellows, research staff, and faculty. This highly interactive institution operates on a dynamic collaborative approach toward scientific discoveries and high-quality education. To continue our faculty’s historic interdisciplinary collaborative research ventures through our shared core research facilities, we have identified an urgent need for a Zeiss LSM 980 confocal microscope system with the latest Airyscan 2 technology. This advanced quantitative fluorescence microscopy is capable of fast image acquisition with ultrahigh spatial resolution and multispectral imaging at high spectral resolution. Acquisition of this advanced imaging platform will facilitate a wide variety of research programs across our major and minor user groups encompassing diverse fields including stem cell biology; cell-cell communication in organ development; neuronal development and behavior; microbial communities in human health; gene regulation and transcriptional activation; epitranscriptomic regulation by viral infection, environmental, and physical stress; protein biosynthesis and translational regulation. The most advanced imaging systems currently available at UAlbany, the conventional confocal Zeiss LSM710 fluorescence microscopy and two-photon fluorescence microscope, lack sufficient resolution, sensitivity and speed to support these multidisciplinary cutting-edge research programs. Our major users, in particular, require the Zeiss 980 superior resolution and imaging capacity to answer critical research questions that are central to their research programs, including: 1) understanding the interactions between niche cells and stem cell daughters responsible for cell differentiation; 2) defining the cell-cell and cell-ECM interactions that regulate organ-formation and regeneration in disease; 3) understanding the development and regeneration of sensory neurons and GnRH-1 neurons in homeostasis and disease, and 4) defining systems level structure-function relationships in the oral microbiome. The imaging system is also critical to support the diverse research programs of our many minor users to acquire multispectral data, high resolution images, and fast time-lapse images to answer critical research questions. The excellent upgrade capabilities of this system will enable our program’s major and minor users as well as the broader research community of New York’s capital region to meet the imaging research needs of their cutting-edge research programs.