Zeiss LSM 980 Confocal Microscope System

项目摘要/摘要 生命科学倡议和纽约州立大学奥尔巴尼分校(UAlbany)RNA研究所 代表本科生和研究生进行多学科协作研究的区域中心 学生、博士后研究员、研究人员和教职员工。这个高度互动的机构在 科学发现和高质量教育的动态协作方法。为了继续我们的 通过我们共享的核心研究设施，实现教职员工历史性的跨学科协作研究， 我们发现急需蔡司LSM 980共焦显微镜系统和最新的Airyscan2 技术这种先进的定量荧光显微镜能够快速采集图像， 超高空间分辨率和高光谱分辨率的多光谱成像。收购这一先进的 影像平台将促进我们主要和次要用户群体的各种研究计划 涵盖多个领域，包括干细胞生物学；器官发育中的细胞-细胞通讯； 神经元发育和行为；人类健康中的微生物群落；基因调控和 转录激活；病毒感染、环境和身体应激对表位转录的调节； 蛋白质的生物合成和翻译调控。目前最先进的成像系统，请访问 UAlbany，常规共焦蔡司LSM710荧光显微镜和双光子荧光 显微镜缺乏足够的分辨率、灵敏度和速度来支持这些多学科的前沿 研究项目。我们的主要用户尤其需要蔡司980卓越的分辨率和成像能力 回答对其研究计划至关重要的关键研究问题的能力，包括：1) 了解负责细胞分化的干细胞子体和小生境细胞之间的相互作用； 2)明确调节疾病中器官形成和再生的细胞-细胞和细胞-细胞外基质的相互作用；3) 理解动态平衡中感觉神经元和促性腺激素释放激素释放激素-1神经元的发育和再生 和疾病，以及4)定义口腔微生物组中的系统级结构-功能关系。成像 系统也是支持我们许多次要用户获取的多样化研究计划的关键 多光谱数据、高分辨率图像和快速延时图像，以回答关键的研究问题。 该系统出色的升级能力将使我们程序的主要用户和次要用户以及 纽约首都地区更广泛的研究社区，以满足其 尖端研究项目。

Project Summary/Abstract The Life Science Initiative and the RNA Institute of the State University of New York at Albany (UAlbany) represent a regional hub for multidisciplinary and collaborative research by undergraduate and graduate students, post-doctoral fellows, research staff, and faculty. This highly interactive institution operates on a dynamic collaborative approach toward scientific discoveries and high-quality education. To continue our faculty’s historic interdisciplinary collaborative research ventures through our shared core research facilities, we have identified an urgent need for a Zeiss LSM 980 confocal microscope system with the latest Airyscan 2 technology. This advanced quantitative fluorescence microscopy is capable of fast image acquisition with ultrahigh spatial resolution and multispectral imaging at high spectral resolution. Acquisition of this advanced imaging platform will facilitate a wide variety of research programs across our major and minor user groups encompassing diverse fields including stem cell biology; cell-cell communication in organ development; neuronal development and behavior; microbial communities in human health; gene regulation and transcriptional activation; epitranscriptomic regulation by viral infection, environmental, and physical stress; protein biosynthesis and translational regulation. The most advanced imaging systems currently available at UAlbany, the conventional confocal Zeiss LSM710 fluorescence microscopy and two-photon fluorescence microscope, lack sufficient resolution, sensitivity and speed to support these multidisciplinary cutting-edge research programs. Our major users, in particular, require the Zeiss 980 superior resolution and imaging capacity to answer critical research questions that are central to their research programs, including: 1) understanding the interactions between niche cells and stem cell daughters responsible for cell differentiation; 2) defining the cell-cell and cell-ECM interactions that regulate organ-formation and regeneration in disease; 3) understanding the development and regeneration of sensory neurons and GnRH-1 neurons in homeostasis and disease, and 4) defining systems level structure-function relationships in the oral microbiome. The imaging system is also critical to support the diverse research programs of our many minor users to acquire multispectral data, high resolution images, and fast time-lapse images to answer critical research questions. The excellent upgrade capabilities of this system will enable our program’s major and minor users as well as the broader research community of New York’s capital region to meet the imaging research needs of their cutting-edge research programs.