Two-photon laser-scanning microscope for interdisciplinary collaborations at Weill Cornell

项目摘要 这是一个由神经生理学，神经发育，免疫学， 微生物学和神经退行性疾病，以获得共享仪器赠款， Olympus Fluoview多光子激发共振扫描（FVMPE-RS）显微镜 配备两台Spectra-Physics Ti：Sapphire超快激光器。奥林巴斯FVMPE-RS是一款 创新的，最先进的双光子（2 P）激光扫描显微镜，允许 同时在两个不同的波长荧光团激发和成像， 设计用于神经科学中的体内成像应用。通过允许并发 具有可辨别发射光谱的两种遗传编码的钙传感器的激发 在不同的神经元亚型中独立表达，可以评估激活 以及不同神经元网络之间的相互作用。特别设计的 龙门系统允许显微镜物镜和 基板，使用户能够安装定制的行为设备，特别是复杂的 行为测定与体内成像同时进行。同样的设备将 还允许同时进行2 P成像和2 P光遗传学刺激， 研究人员不仅要记录神经元网络，还要操纵它们的活动， 真实的时间，单细胞精度。所要求的仪器将使威尔康奈尔 研究人员进行跨学科的实验，包括神经生理学，发育， 神经生物学、免疫学和微生物学。

Project Summary This is a proposal by investigators in neurophysiology, neurodevelopment, immunology, microbiology, and neurodegeneration for a Shared Instrumentation Grant to purchase an Olympus Fluoview Multiphoton Excitation Resonance Scanning (FVMPE-RS) Microscope equipped with two Spectra-Physics Ti:Sapphire Ultrafast Lasers. The Olympus FVMPE-RS is an innovative, state-of-the-art two-photon (2P) laser-scanning microscope that allows for simultaneous fluorophore excitation and imaging at two different wavelengths and is specially designed for in vivo imaging applications in neuroscience. By permitting the concurrent excitation of two genetically encoded calcium sensors with discernible emission spectra expressed independently in different neuronal subtypes, it is possible to assess the activation and interaction of different neuronal networks in behaving animals. The specially designed gantry system allows for 355 mm of clearance between the microscope objective and the baseplate, enabling users to install customized behavioral equipment for especially complex behavioral assays to be performed concurrently with in vivo imaging. The same equipment will also allow for simultaneous 2P imaging and 2P optogenetic stimulation, enabling the investigators not only to record from neuronal networks but also to manipulate their activity in real time, with single-cell precision. The requested instrument would enable Weill Cornell investigators to conduct multidisciplinary experiments spanning neurophysiology, developmental neurobiology, immunology, and microbiology.