Elyra 7 Microscope with Lattice SIM2

带 Lattice SIM2 的 Elyra 7 显微镜

• 批准号: 10431347
• 负责人: Binhai Zheng
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431347
• 关键词: 3-Dimensional; Algorithms; Animal Model; Area; Biomedical Research; Cardiovascular system; Communities; Core Facility; Core Grant; Diabetes Mellitus; Disease; Ensure; Face; Faculty; Funding; Image; Inflammatory; Infrastructure; Laser Scanning Confocal Microscopy; Leadership; Light; Lighting; Lung; Malignant Neoplasms; Mental disorders; Metabolic; Microscope; Microscopy; Modality; Phase; Research; Research Personnel; Resolution; Sampling; Scanning; Site; Slide; Specimen; Speed; Structure; System; Technology; Thick; United States National Institutes of Health; base; faculty support; flexibility; image reconstruction; imaging system; improved; innovation; instrument; instrumentation; light microscopy; live cell imaging; medical schools; nervous system disorder; programs; reconstruction

PROJECT SUMMARY / ABSTRACT The UCSD School of Medicine Microscopy Core is the largest light microscopy core facility on campus and has been serving UCSD researchers for 18 years. It is accessible by all UCSD researchers regardless of department affiliation and currently has over 200 registered user labs across the campus. The Core enjoys strong support from the faculty and our institutional leadership. The current array of 12 instruments at the Core includes multiple confocal systems, multiphoton, light sheet, Gatan 3View serial block face scanning EM, slide scanning, as well as others. A critical need exists in the realm of cutting-edge super-resolution live cell imaging, which is currently lacking locally. At the moment, our only super-resolution systems are point scanning confocal platforms which are slow and heavily photo-bleach samples. Here we seek funding to acquire an Elyra 7 system with Lattice SIM2 from Zeiss as our primary super-resolution live cell imaging system. This system can also be used to image developing model organisms and thick fixed specimens. The groundbreaking 2-fold increase in sampling efficiency over classic SIM (Structured Illumination Microscopy) enables fast, gentle 3D super-resolution imaging at penetrating depths on par with point scanning confocal microscopy. Our on-site instrument demonstration validated the initial Elyra 7 system with Lattice SIM (Version 1) as a practical solution for super-resolution live cell imaging, which also comes with some innovative processing modalities that further enhance speed and versatility. With the recent introduction of the dual iterative reconstruction algorithm, the newly upgraded Elyra 7 Lattice SIM2 has substantially improved the resolution of the system from 120 nm XY and 300 nm Z to a remarkable 60 nm XY and 200 nm Z. The upgraded SIM2 system is also more flexible requiring fewer phase shifts for image reconstruction. Our proposed user labs illustrate a critical need among the local investigators for the proposed system in advancing their NIH-funded research. They come from all corners of the campus, and their research programs collectively cover a range of biomedical research fields with many disease areas represented, including cancer, diabetes, cardiovascular, pulmonary, metabolic, infectious, inflammatory, neurological and psychiatric diseases among others. If funded, the Elyra 7 Lattice SIM2 technology platform will be extensively utilized by proposed users and additionally will reach a potentially much larger user base as our core has proven capable of disseminating cutting-edge microscopy technologies to the wide local research community. Adequate space and infrastructure, capable staff, robust faculty oversight, and strong institutional commitment along with a track record of program-leading core grant citations will ensure the effective and efficient use of the proposed instrument for years to come. The Elyra 7 Lattice SIM2 system will fill a current void in our instrumentation portfolio and generate a sustained, powerful influence on the biomedical research portfolio at UC San Diego.

项目概要/摘要 加州大学圣地亚哥分校医学院显微镜核心设施是校园内最大的光学显微镜核心设施，拥有 为 UCSD 研究人员服务了 18 年。所有 UCSD 研究人员都可以访问它，无论 部门隶属关系，目前在校园内拥有 200 多个注册用户实验室。核心享受 来自教师和我们机构领导的大力支持。目前核心有 12 种仪器 包括多个共焦系统、多光子、光片、Gatan 3View 串行块面扫描 EM、载玻片 扫描，以及其他。尖端超分辨率活细胞领域存在迫切需求 目前国内尚缺乏影像学检查。目前，我们唯一的超分辨率系统是点 扫描共焦平台，这是缓慢且严重光漂白的样品。我们在这里寻求资金 购买配备蔡司 Lattice SIM2 的 Elyra 7 系统作为我们的主要超分辨率活细胞成像 系统。该系统还可用于对发育模型生物和厚固定标本进行成像。这 与传统 SIM（结构照明显微镜）相比，采样效率突破性提高 2 倍 可在穿透深度实现快速、温和的 3D 超分辨率成像，与点扫描共焦相当 显微镜。我们的现场仪器演示验证了带有 Lattice SIM 的初始 Elyra 7 系统（版本 1）作为超分辨率活细胞成像的实用解决方案，它还带有一些创新 进一步提高速度和多功能性的处理方式。随着最近推出的双 迭代重建算法，新升级的Elyra 7 Lattice SIM2大幅提升了 系统的分辨率从 120 nm XY 和 300 nm Z 到卓越的 60 nm XY 和 200 nm Z。 升级后的 SIM2 系统也更加灵活，图像重建所需的相移更少。我们的 拟议的用户实验室说明了当地研究人员对拟议系统的迫切需求 他们由 NIH 资助的研究。他们来自校园的各个角落，他们的研究项目集体 涵盖一系列生物医学研究领域，涉及许多疾病领域，包括癌症、糖尿病、 心血管、肺部、代谢、感染、炎症、神经和精神疾病 其他的。如果获得资助，Elyra 7 Lattice SIM2 技术平台将被提议的用户广泛使用 此外，由于我们的核心已被证明有能力传播，因此将覆盖潜在的更大的用户群 向广泛的当地研究界提供尖端的显微镜技术。足够的空间和 基础设施、有能力的员工、强有力的教师监督、强有力的机构承诺以及轨道 计划主导的核心赠款引用记录将确保有效和高效地使用拟议的 未来几年的乐器。 Elyra 7 Lattice SIM2 系统将填补我们仪器中当前的空白 投资组合并对加州大学圣地亚哥分校的生物医学研究组合产生持续、强大的影响。